FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355353
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43690.32235144644974406No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA41910.3092183364776556No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37590.27734472126449716No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAGGAAGATGCTGGCAAAGGATGG36020.2657610231430484No Hit
GTCTACAAACTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA35170.25948959422379264No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGACTAGAACCCAAATA33290.2456186690847329No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT29950.22097564250789276No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCATGAATAGCAACGGATACAACTATTTG29840.22016404582422439No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29270.21595849937248818No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC29190.21536824723891118No Hit
GGTCTAGTCAGAGCCTCATGAATAGCAACGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC26440.1950783301472015No Hit
GTTTGTAGACCTTGCATGCAGTAATAAATCCCAACATCTTCAGCCTCCACTCTGCTGATTATCAGTGTAAAAT25880.19094656521216244No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA25670.1893971533615228No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTG25130.18541295145987796No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT24530.1809860604580504No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA24180.178403707373651No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC24180.178403707373651No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24070.17759211068998262No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22980.16954992536999586No Hit
GATTTTACACTGATAATCAGCAGAGTGGAGGCTGAAGATGTTGGGATTTATTACTGCATGCAAGGTCTACAAACT22920.1691072362698131No Hit
GTATGTGGTAGCACCATTACCAAAAATAATTGAGACCCACTCTAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT22660.16718891683568785No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC22550.16637732015201942No Hit
CTCCCTACCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCATGAATAGCAACG21750.16047479881624935No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA21310.15722841208157579No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20890.1541295883802965No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20610.152063705912777No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGA20320.14992404192856032No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTC20220.14918622676158905No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20140.14859597462801205No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19920.14697278126067526No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA19250.1420294196419678No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT18500.13649580588968335No Hit
GGACTGAACAGAGAGTACTCACCATGGAGTTTGGGCTGAGCTGCCTTTTT18430.13597933527280348No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG18260.1347250494889523No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18130.1337658897718897No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC18120.13369210825519257No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA17550.12948656180345636No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCA17070.1259450490019943No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16950.1250596708016288No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTCCAAGGACGTCATGCAGGGCACAGAC16800.12395294805117191No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGAT16310.12033765373301274No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15930.11753395609852194No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15900.11731261154843055No Hit
GTAATAAATCCCAACATCTTCAGCCTCCACTCTGCTGATTATCAGTGTAAAATCTGTGCCTGATCCACTGCCA15880.1171650485150363No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGATAATCAGCAGAGTGGAGGCTGAAGAT15840.1168699224482478No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15750.11620588879797367No Hit
GTCAGGGACCCCGGAGGCCCGACTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA15350.11325462813008862No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACCCAGTCTCCACTCTCCCTACCCGTCACCC15310.1129595020633001No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACACACATGGAGGACGCATTCTGCTGGAA15210.11222168689632886No Hit
CTCTAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGGAATAGGTGCTGAAGGTGAATCCAGAGGCTGCACA15150.11177899779614611No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTG14940.11022958594550646No Hit
GATAATCAGCAGAGTGGAGGCTGAAGATGTTGGGATTTATTACTGCATGCAAGGTCTACAAACTCCTCTCACTTT14870.10971311532862657No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC14280.10536000584349613No Hit
CTCCAGGGGTGACGGGTAGGGAGAGTGGAGACTGGGTCATCACAATATCC14220.10491731674331335No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAGTCGGGCCTCCGGGGTCCCTGACAGGTTCAGTG14170.10454840915982773No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT14170.10454840915982773No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14060.10373681247615935No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14040.1035892494427651No Hit
ACCACATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAGAAATATAGTATATCTC14030.10351546792606797No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC14020.10344168640937085No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGATAATCAGCAGAG13910.10263008972570245No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT13880.10240874517561108No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCGTTGCTATTCATGAGGCTCTGACTAGACCTGCAG13720.10122824090845707No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)