FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709989
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23500.3309910435232095No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT20800.2929622853311812No Hit
GAGTTACAGTACCCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA19190.2702858776685273No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16360.23042610519317908No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT14810.20859478104590354No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14700.20704546126770976No Hit
GTCTCTGGTGGCTCCATCAGCAATAGTAATTACTATTGGGGCTGGATCCGCCAGTCCCCAGGGAAGGGACTGGAG14450.2035242799536331No Hit
CCGTAATACCCCCCAAGTCTCTCCCTCCCACAGTAATACATGGCCGTGTC14330.20183411292287626No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14330.20183411292287626No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14110.19873547336648878No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14100.1985946261139257No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13990.19704530633573195No Hit
GTCTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGT13420.18901701293963707No Hit
CAGTAATACATGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGA12690.17873516350253313No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG11710.1649321327513525No Hit
GTATTACTGTGGGAGGGAGAGACTTGGGGGGTATTACGGTGACCACGAAGTCTACTACTACGGTATGGACGTC11710.1649321327513525No Hit
GTATTACGGTGACCACGAAGTCTACTACTACGGTATGGACGTCTGGGGCC11700.1647912854987894No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11690.16465043824622635No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT11670.16436874374110022No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11650.1640870492359741No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11550.1626785767103434No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT11470.16155179868983888No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11230.15817146462832524No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11060.15577706133475308No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG10800.1521150327681133No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC10350.14577690640277524No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT10320.14535436464508605No Hit
GATTAATGGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGTGGGGTCCCATCAAGGTTCCGTGG10190.14352335036176617No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10120.14253741959382468No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9980.14056555805794174No Hit
GTAGTGGATCTGCGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCT9410.13253726466184687No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8930.12577659653881962No Hit
GTAGTAGACTTCGTGGTCACCGTAATACCCCCCAAGTCTCTCCCTCCCAC8760.12338219324524746No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8700.12253710972986906No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8600.12112863720423836No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC8600.12112863720423836No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8490.11957931742604462No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA8370.11788915039528781No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8320.11718491413247246No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG8300.11690321962734634No Hit
CCATTAATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAATAGTTGTTAATGCTCTGACT8280.11662152512222021No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC7880.11098763501969748No Hit
GTTCAGTGGTGGGGTCCCATCAAGGTTCCGTGGTAGTGGATCTGCGACAGATTTCACTCTCACCATCAGCAG7820.11014255150431909No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7820.11014255150431909No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7760.10929746798894067No Hit
CTGTAACTCTGTTGACAGAAATAAGTTGCAAAATCTTCAGGTTGCAGACT7760.10929746798894067No Hit
GTAATACATGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGAAAACTGGTTCCTGGACGTGTCTACGGA7710.10859323172612532No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7700.10845238447356226No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7580.10676221744280545No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG7560.10648052293767932No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG7540.1061988284325532No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7510.10577628667486397No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGGGAGGGGTA7490.10549459216973785No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC7440.1047903559069225No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7300.10281849437103956No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7260.10225510536078727No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGA7160.1008466328351566No Hit
ACAGTACCCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCA7140.10056493833003047No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAATCACCATCACTTGCCGGGCAAGTCAGAGCATTAACAA7140.10056493833003047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)