FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156016
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACTATACTAACTCACCTCGAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTG52300.45241588351718315No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT36360.31452851863642023No Hit
ATACTAACTCACCTCGAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCT34920.3020719436409185No Hit
CAGTAATACATGGCCGTGTCTGCAGCGGTCACAGAGCTGAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTA34120.295151624198973No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA32420.28044594538483897No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32100.27767781760806076No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG28850.24956401987515747No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26250.22707298168883477No Hit
GTATTACTGTGCGCGCATGTTAACAGTGGGCCTTGGGGGATACCACTTTGACTACTGGGGCCAGGGAACCCTGG25950.2244778618981052No Hit
GAGTTAGTATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAG25190.21790355842825704No Hit
GTAATACATGGCCGTGTCTGCAGCGGTCACAGAGCTGAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA23450.20285186364202573No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT23320.20172731173270958No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22620.19567203222100732No Hit
GTGTATTACTGTCAGCACTATACTAACTCACCTCGAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA21320.18442651312784597No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20330.17586261781843848No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAACTACTTAGCCTGGTACC19780.171104898202101No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19410.16790425046020124No Hit
GTATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTT19330.1672122185160067No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19200.16608766660669055No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC19200.16608766660669055No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18820.16280051487176647No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18390.1590808431717208No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC18130.15683173935308856No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAG18010.15579369143679672No Hit
GTCTCTGGTGGCTCCATCAATAATTACTACTGGAGTTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGCACAT17850.15440962754840762No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17780.1538040995972374No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17690.15302556366001854No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17290.14956540393904583No Hit
GCACTATACTAACTCACCTCGAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA17120.1480948360576324No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17070.14766231609251085No Hit
CGTCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA16950.146624268176219No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16670.1442021563715381No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGACAGCTCCCAGATGGGTC16660.1441156523785138No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16260.14065549265754107No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCACTA16200.14013646869939517No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16010.1384928928319331No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15690.13572476505515496No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15570.13468671713886313No Hit
GAGTGTTACCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG15210.13157257338998768No Hit
GTATAATAGATATATCCAATGTGCTCCAGTCCCTTCCCTGGGGGCTGCCG15190.13139956540393904No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGACGAGGACCC15040.1301020055085743No Hit
CCATAGACGAGGACCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGTTGCTGGTAACACTCTGA14960.12940997356437972No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT14900.12889094960623382No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAACTACTTAGCC14760.12767989370389338No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT14440.12491176592711518No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14030.12136510221311815No Hit
GTCAGAGTGTTACCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGGTCCTCGTCTA13830.1196350223526318No Hit
ATGTATTACTGTGCGCGCATGTTAACAGTGGGCCTTGGGGGATACCACTTTGACTACTGGGGCCAGGGAACCC13570.11738591853399953No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13550.11721291054795088No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA13370.11565583867351317No Hit
CTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC13050.11288771089673501No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12960.11210917495951614No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12890.11150364700834592No Hit
CTGTTAACATGCGCGCACAGTAATACATGGCCGTGTCTGCAGCGGTCACAGAGCTGAGCTTCAGGGAGAACTGGT12860.11124413502927295No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC12710.10994657513390818No Hit
TGTTAACAGTGGGCCTTGGGGGATACCACTTTGACTACTGGGGCCAGGGA12610.10908153520366501No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC12420.10743795933620295No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12280.10622690343386251No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG11980.10363178364313297No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11780.1019017037826466No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGA11770.10181519978962228No Hit
GACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCACTATACTAACTCA11760.10172869579659796No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCC11580.10017162392216025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGA450.002331546369.16933470
GTTATTA803.4851997E-451.87700370
ATATCGT507.4222044E-847.89420337
GGAAACG1102.9454643E-547.1609170
TCGTATG2700.045.7473640
CTTATAC3050.044.4638561
TATCGTA551.5597834E-743.5782438
GTATGCC2850.043.4251942
TATACAC3100.042.6308943
GGTACGG405.55958E-542.3594474
GGTATCA7300.042.2635041
ATGCCGT2950.042.0306144
GCCGTCT3050.041.8517146
CGTATGC2800.041.69930341
GTATCAA23050.040.8903541
TGCCGTC3050.040.68654345
TATCTCG2400.039.87178836
GCGTAGA20650.039.69281470
ATACACA3600.039.535494
TTATACA3450.039.2882272
Produced by FastQC (version 0.11.5)