FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156016
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACTATACTAACTCACCTCGAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGT47990.4151326625237021No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37670.3258605417226059No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36070.31201990283871506No Hit
ATACTAACTCACCTCGAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA35560.3076081991944748No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA32280.2792348894824985No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAGGAAGATGCTGGCAAAGGATGG29970.25925246709388106No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA29020.25103458775657084No Hit
CAGTAATACATGGCCGTGTCTGCAGCGGTCACAGAGCTGAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTA28840.24947751588213313No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA24250.20977218308397116No Hit
GAGTTAGTATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGT24250.20977218308397116No Hit
GTATTACTGTGCGCGCATGTTAACAGTGGGCCTTGGGGGATACCACTTTGACTACTGGGGCCAGGGAACCCTGGT23050.199391703921053No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT22550.1950665042698371No Hit
GTAATACATGGCCGTGTCTGCAGCGGTCACAGAGCTGAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACT21750.18814618482789167No Hit
GTGTATTACTGTCAGCACTATACTAACTCACCTCGAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA20750.17949578552545986No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20130.17413253795795214No Hit
GTATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCA19910.17222945011141716No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC19700.17041286625790647No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19190.16600116261366626No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19100.16522262667644738No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC18390.1590808431717208No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18330.1585618192135749No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17770.15371759560421308No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAG17770.15371759560421308No Hit
GTCTCTGGTGGCTCCATCAATAATTACTACTGGAGTTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGCACATT17740.15345808362514013No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCA17410.15060345185533766No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGACAGCTCCCAGATGGGTC17410.15060345185533766No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA17100.14792182807158377No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA17080.14774882008553517No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17040.14740280411343787No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16660.1441156523785138No Hit
GCACTATACTAACTCACCTCGAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT16440.1422125645319788No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATT16230.14039598067846812No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT16120.13944443675520063No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGA15610.1350327331109604No Hit
CCATAGACGAGGACCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGTTGCTGGTAACACTCT15010.1298424935295013No Hit
CGTCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA14960.12940997356437972No Hit
GTATAATAGATATATCCAATGTGCTCCAGTCCCTTCCCTGGGGGCTGCCGGATCCAACTCCAGTAGTAATTATT14910.12897745359925816No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14640.12664184578760157No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGACGAGGACCCTG14360.12421973398292065No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14210.12292217408755587No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTC14180.12266266210848294No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13900.12024055030380203No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAACTACTTAGCCTG13720.11868347842936429No Hit
GAGTGTTACCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG13530.11703990256190226No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA13200.11418527079209977No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13150.11375275082697817No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13130.11357974284092955No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACACACATGGAGGACGCATTCTGCTGGAA13080.11314722287580795No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA12970.11219567895254046No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATA12690.10977356714785955No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12460.10778397530830024No Hit
ATGTATTACTGTGCGCGCATGTTAACAGTGGGCCTTGGGGGATACCACTTTGACTACTGGGGCCAGGGAACCCTG12430.10752446332922727No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12360.10691893537805705No Hit
GTCAGAGTGTTACCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGGTCCTCGTCTATG12320.10657291940595978No Hit
CTGTTAACATGCGCGCACAGTAATACATGGCCGTGTCTGCAGCGGTCACA12240.10588088746176524No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTCCAAGGACGTCATGCAGGGCACAGAC12040.10415080760127887No Hit
CTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC12030.10406430360825455No Hit
GACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCACTATACTAACTCA11830.10233422374776821No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC11610.10043113590123319No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTT11570.10008511992913594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA2900.0108.7311370
GTAGTTT1001.6892036E-460.06100570
CTGTCTT35200.044.36324370
CGTCACA10250.043.9470870
TATTCTA1806.07787E-541.7090370
GGTATCA9250.041.0591241
GAGCAAA17400.040.55843470
CCGACAG350.001470728538.7346615
GCGGGAA15450.037.90257370
ACCGGTA5650.037.20593370
GTATCAA22850.036.6559221
GCACATT8350.036.09268669
GCCGGTT1800.003053679133.36722670
AAGGACA18250.032.91013770
TATCAAC26250.031.88052
AGTTGTT10000.031.53202870
ATCAACG27200.030.5126233
TCAACGC27400.030.2938334
CCTGCTT9950.030.1814170
ATACGGG450.005050617330.1165317
Produced by FastQC (version 0.11.5)