FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643082
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28820.4481543566761315No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT26650.4144106039354235No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24300.37786783023004844No Hit
GTATAATCTTTATTCTTGGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCCAACGAACTGTGGCTGCACCA24200.3763128185830112No Hit
CTCTAAAGTAGACGCCTTAGAGATCAGGAGCTTAGGGGCCTTCCCTGGTT22610.35158813339511913No Hit
ACTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGACAGAATTCGCTCTCACCATCAGCAGC21000.3265524458778196No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCCAACGAACTGTGGCTGCACCA19210.2987177373958531No Hit
GAATATTGGTGACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAGGCCCCTAAGCTCCTGATCTCTAAGGCG18660.29016517333714825No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17900.2783470848196653No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT17750.27601456734910945No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17490.2719715370668126No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17300.26901701493744185No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17030.26481848349044135No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16200.2519118868200323No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15540.2416488099495865No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT15460.24040480063195674No Hit
CCAATATTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA13720.21334759797350883No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC13550.21070407817354553No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13490.20977107118532318No Hit
AGATTATACTGTTGACAATAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGCGAATTCTGTC13260.20619454439713752No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12950.2013740082913221No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12850.19981899664428485No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12830.19950799431487742No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTGGTGACTGG12580.19562046519728432No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12430.19328794772672847No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTGGTGACTGGTTGGCCTGGTA11350.17649382193872634No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11280.17540531378580027No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC10900.16949626952705876No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTGGTGACTGGTTGGCCTGGTATCAGCAGAAA10870.1690297660329476No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACA10830.1684077613741327No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10450.16249871711539118No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10310.16032170080953906No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9930.15441265655079756No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATG9910.15410165422139013No Hit
CCTTAGAGATCAGGAGCTTAGGGGCCTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTCACCAATATTCTGA9130.14197256337449968No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9060.1408840552215736No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG8870.13792953309220288No Hit
GGCCAAAACTGTACCAAGAATAAAGATTATACTGTTGACAATAATAAGTTGCAAAATCATCAGGCTGCAGGC8830.13730752843338798No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG8720.135597015621647No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8510.1323314911628688No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8410.13077647951583157No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8400.13062097835112785No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC8190.12735545389234967No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8190.12735545389234967No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8090.12580044224531242No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8040.1250229364217938No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7930.12331242361005285No Hit
TCTCTAAGGCGTCTACTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGACAGAATTCGC7910.12300142128064538No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7840.12191291312771933No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7840.12191291312771933No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7770.12082440497479326No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT7760.12066890381008955No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7720.12004689915127463No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGGATCTCCAGCTTGGTCC7710.11989139798657092No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7620.11849188750423742No Hit
TCTTTATTCTTGGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCCAACGAACTGTGGCTGCACCATCTGTCT7580.1178698828454225No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG7570.1177143816807188No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7490.116470372363089No Hit
GTGCAGCCACAGTTCGTTGGATCTCCAGCTTGGTCCCCTGGCCAAAACTGTACCAAGAATAAAGATTATACTGT7440.11569286653957038No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7440.11569286653957038No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7400.11507086188075549No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG7340.11413785489253314No Hit
GTCTCTGGTGTCTCCATCAGCAGTCGTGAATGGTGGAGTTGGGTCCGCCAGCCCCCAGGGAAGGGGCTGGAG7270.11304934673960708No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT7220.11227184091608847No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7170.11149433509256984No Hit
GTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCCAACGAACTGTGGCTG7080.11009482461023634No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6750.10496328617501345No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT6720.10449678268090229No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6710.10434128151619855No Hit
ACCTTGATGGGACCCCACTCTCTAAAGTAGACGCCTTAGAGATCAGGAGCTTAGGGGCCTTCCCTGGTTTC6630.10309727219856876No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTGGTGA6620.10294177103386505No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA6560.10200876404564271No Hit
GCTTAGGGGCCTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTCACCAATATTCTGACTGGCCCGGCAAGTGA6540.10169776171623525No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6510.10123125822212409No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6460.10045375239860546No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA6440.10014275006919801No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACT150.002450932368.3091238
CGGCAAA500.003977739760.437970
TGTTAGA8200.057.7353970
TCGTATG1100.052.92576240
ATCTCGT1050.048.7309537
CGTATGC1150.047.7294741
AGACCGA1150.047.0753925
TAGCGAG305.796837E-446.7973556
CTTTCGA508.158922E-443.6567669
CCGTCTT1350.043.56414447
TCTCCGA1400.043.4418211
CACGAGA1350.042.57443621
CCCACGA1350.042.57112519
TATACAC1550.041.4240883
TGCTTGA1550.040.68996455
GCGTAGA15600.040.67935670
TACACAT1500.040.5520025
AATGGTA2251.1277734E-940.2919370
ATCTCCG1600.040.12334410
ACATCTC1600.040.1233448
Produced by FastQC (version 0.11.5)