FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643082
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA26470.4116115829707565No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24560.38191086051234524No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22550.3506551264068968No Hit
GTATAATCTTTATTCTTGGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCCAACGAACTGTGGCTGCACCAT21870.3400810472070436No Hit
ACTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGACAGAATTCGCTCTCACCATCAGCAGC20060.3119353363956696No Hit
CTCTAAAGTAGACGCCTTAGAGATCAGGAGCTTAGGGGCCTTCCCTGGTT19690.30618179330163187No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC18640.28985417100774086No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT17570.2732155463844424No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT17120.26621799397277485No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA16600.25813193340818125No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16330.2539334019611807No Hit
GAATATTGGTGACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAGGCCCCTAAGCTCCTGATCTCTAAGGCGT14860.23107473074973334No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14770.22967522026739984No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14540.22609869347921416No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA13840.2152136119499535No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13590.21132608283236043No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13470.20946006885591575No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA13160.20463953275010027No Hit
AGATTATACTGTTGACAATAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGCGAATTCTGTC12870.20012999897369232No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC12620.19624246985609925No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12540.19499846053846942No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA11650.18115885687983804No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC11620.18069235338572684No Hit
CCAATATTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGG11440.17789333242105984No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11300.17571631611520772No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10710.166541747397688No Hit
GGCCAAAACTGTACCAAGAATAAAGATTATACTGTTGACAATAATAAGTTGCAAAATCATCAGGCTGCAGGCT10480.1629652206095024No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10380.16141020896246513No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10360.16109920663305768No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10350.16094370546835396No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA10290.16001069848013164No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT9980.15519016237431618No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG9810.15254664257435288No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT9790.15223564024494543No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGT9750.15161363558613056No Hit
CCTTAGAGATCAGGAGCTTAGGGGCCTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTCACCAATATTCT9240.14368307618624063No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9070.14103955638627733No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8830.13730752843338798No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8800.1368410249392768No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8770.13637452144516563No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC8620.13404200397460977No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTGGTGACTGGTTGGCCTGGTATCAGCAGAAA8540.13279799465697997No Hit
GTGCAGCCACAGTTCGTTGGATCTCCAGCTTGGTCCCCTGGCCAAAACTGTACCAAGAATAAAGATTATACTGTT8420.1309319806805353No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT8270.12859946320997942No Hit
GAATAAAGATTATACTGTTGACAATAATAAGTTGCAAAATCATCAGGCTG8100.12595594341001615No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA7920.12315692244534912No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7890.12269041895123796No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7880.12253491778653422No Hit
TCTTTATTCTTGGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCCAACGAACTGTGGCTGCACCATCTGTCTT7730.12020240031597837No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7630.11864738866894113No Hit
GTCTCTGGTGTCTCCATCAGCAGTCGTGAATGGTGGAGTTGGGTCCGCCAGCCCCCAGGGAAGGGGCTGGAGTG7510.11678137469249646No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7380.11475985955134804No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA7300.11351585023371825No Hit
TCTCTAAGGCGTCTACTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGACAGAATTCGCT7120.11071682926905121No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7110.1105613281043475No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7110.1105613281043475No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGGATCTCCAGCTTGGTCC7050.10962832111612517No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7020.10916181762201399No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6930.10776230713968046No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC6840.10636279665734696No Hit
GTCCTGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC6640.1032527733632725No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGA6590.10247526753975388No Hit
GAATTCGCTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA6550.10185326288093897No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6500.10107575705742038No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTG6460.10045375239860546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)