FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048492
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG32060.30577248085822306No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT31010.2957580982973642No Hit
GCAATATACGGCCGTGTCCTCGACTCTCAGGCCGTTCATTTGCAAATACA29400.28040271170404735No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG28570.2724865807273684No Hit
CCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG28540.2722004555113439No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC27290.26027857151032147No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAG26560.2533161912537244No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT25930.2473075617172091No Hit
TCTATAGGGATAGCAACCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGCCACC24670.2352903026441785No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG24310.23185680005188403No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG22940.21879041518676348No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22410.21373553637032996No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAG22290.21259103550623182No Hit
GGATTACCTGTGGGGGACACAACATTGGAAGTAAAAATGTGCACTGGTATCAACAGAAGCCAGGCCAGGCCC22050.2103020337780355No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC21310.20324427844943022No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT20170.19237152024049778No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC19830.18912876779221968No Hit
GACTATTACTGTCAGGTGTGGGACAGCAGCACCGAGCATGTCTTCGCAACTGGGACCAAGGTCACCGTCCTAGG19780.1886518924321788No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTGCGAAGACATGCTCGGTGCTGCTGTCCCACACCTGACAGTAATAG19690.18779351678410516No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG18930.18054501131148354No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18720.17854213479931177No Hit
GGTATCAACAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCTATAGGGAT18320.1747271319189846No Hit
CCCCTGTGCTGGTCATCTATAGGGATAGCAACCGGCCCTCTGGGATCCCT18210.17367800612689463No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18090.17253350526279645No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17510.16700175108632206No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17090.16299599806197854No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGAC16920.1613746218378395No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT16620.1585133696775941No Hit
GTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGG16560.15794111924554502No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC16470.1570827435974714No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16410.15651049316542234No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAGGATTACCTGTGGGGGACACAACATTGGAAGTAAAAATG15740.1501203633408743No Hit
GTGCTGCTGTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCTTG15680.14954811290882525No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG15670.14945273783681706No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTGCGAAGACATGCTCGGTGCTGC15590.14868973726075163No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTGCGAAGACATGCTCGG15580.14859436218874345No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG15420.14706836103661258No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15290.14582848510050625No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTGCGAAGACATGCTCGGTGCTGCTGTCCCACACCTGA15260.14554235988448172No Hit
GCACAGGGGCCTGGCCTGGCTTCTGTTGATACCAGTGCACATTTTTACTTCCAATGTTGTGTCCCCCACAGGTAA15240.14535160974046535No Hit
GACACATACTATGCAGACTCCATGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACACTGTATT14750.14067823121206455No Hit
CTATTACTGTCAGGTGTGGGACAGCAGCACCGAGCATGTCTTCGCAACTG14620.13943835527595824No Hit
ATATACGGCCGTGTCCTCGACTCTCAGGCCGTTCATTTGCAAATACAGTGTGTTCTTGGAATTGTCTCTGGAGA14170.13514647703559016No Hit
GTATGTGTCACCACCACTACCACTAATAGTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGC14020.13371585095546748No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTAATGCTCGGGATACCCGGCTTTCGCACAGCAATATACGGCCG14020.13371585095546748No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC13890.13247597501936115No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTAATGCTCGGGATACCCGGCTT13840.13199909965932025No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG13800.13161759937128753No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13490.12866097213903396No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAG13260.12646734548284583No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT13170.12560896983477224No Hit
GGGTGGCCGTGTTCCCCGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCAGAGGGCCGGTTGCTATCCCTATAGA12920.12322459303456773No Hit
GTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGGCCGTGTTCCC12870.12274771767452684No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTT12830.12236621738649413No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG12700.12112634145038781No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTG12640.12055409101833872No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGC12480.11902808986620785No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC12400.11826508929014241No Hit
GCCGTATATTGCTGTGCGAAAGCCGGGTATCCCGAGCATTACAACTGGTTCGACCCCTGGGGCCAGGGAACCC12290.11721596349805244No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGG12200.11635758784997884No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCC12040.11483158669784795No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC12040.11483158669784795No Hit
GTTGATACCAGTGCACATTTTTACTTCCAATGTTGTGTCCCCCACAGGTAATCCTGGCCGTCTGTCCCAGGGCCA11940.11387783597776617No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATAGGGATAGCAACCGGCCCTCTGG11830.1128287101856762No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC11810.11263796004165982No Hit
CAGTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGT11510.10977670788141446No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCC11500.10968133280940626No Hit
GTATATTGCTGTGCGAAAGCCGGGTATCCCGAGCATTACAACTGGTTCGA11460.10929983252137354No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA11340.1081553316572754No Hit
CTCCATGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACAC11330.10805995658526722No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT11260.10739233108120996No Hit
GCATAGTATGTGTCACCACCACTACCACTAATAGTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC11140.10624783021711182No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG11080.10567557978506273No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC11070.10558020471305456No Hit
CCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGTCAG11040.10529407949703001No Hit
GCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG10990.10481720413698913No Hit
CCCTGGGACAGACGGCCAGGATTACCTGTGGGGGACACAACATTGGAAGT10930.10424495370494004No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT10920.10414957863293187No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC10920.10414957863293187No Hit
GAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAG10890.10386345341690732No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTG10880.10376807834489915No Hit
CCCTATAGATGACCAGCACAGGGGCCTGGCCTGGCTTCTGTTGATACCAGTGCACATTTTTACTTCCAATGTTG10820.10319582791285008No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCCGTGGTGACGCCAGACCCCACCTGCT10780.10281432762481736No Hit
CAGCAGCACCGAGCATGTCTTCGCAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA10670.10176520183272739No Hit
GTGTGGGACAGCAGCACCGAGCATGTCTTCGCAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAAC10640.10147907661670284No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACT10590.10100220125666196No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT10530.10042995082461287No Hit
ATACTATGCAGACTCCATGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACACTGTATTTGCAAA10520.1003345757526047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1750.085.7077970
GCAAGGG1105.740585E-754.54131770
CGCAATA200.007005412652.39812557
CTCACGT200.007632991351.27342235
ACTCACG200.007659108451.2291434
AATGGTA1902.3101165E-1047.36482670
GTAGATA855.6386576E-447.05525670
GTGGTAT1750.044.6231541
CTCTTAT705.18412E-1043.6530841
TATACAC1750.042.6789473
ACCGCAC350.001251317440.02845459
GTGTCCA15750.039.3620970
TCGACGT2000.039.16668329
TCTGGAG15250.038.68559370
TTATACA2050.038.0893022
TCCGAGA11350.037.001670
ACTCGAC2100.035.6610327
CTCGACG2200.035.59245728
CTTATAC2100.035.569181
TCTCGTA1550.035.38007438
Produced by FastQC (version 0.11.5)