FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048492
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31980.30500948028215763No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAGGAAGATGCTGGCAAAGGATGG31820.30348347913002677No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC27020.25770344456610067No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT26720.25484219240585526No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAG26480.25255319067765897No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA25980.24778443707724998No Hit
CCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG24850.23700705394032576No Hit
GCAATATACGGCCGTGTCCTCGACTCTCAGGCCGTTCATTTGCAAATACAGTGTGTTCTTGGAATTGTCTCTGGA24480.2334781762760231No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22440.2140216615863545No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC22150.21125578449811727No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT22090.2106835340660682No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG22080.21058815899406003No Hit
TCTATAGGGATAGCAACCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGCCACC21310.20324427844943022No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG21230.20248127787336478No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT20230.19294377067254684No Hit
GGATTACCTGTGGGGGACACAACATTGGAAGTAAAAATGTGCACTGGTATCAACAGAAGCCAGGCCAGGCCC19900.18979639329627696No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGA19890.18970101822426874No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGG19040.1815941371035735No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG18130.17291500555082917No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTGCGAAGACATGCTCGGTGCTGCTGTCCCACACCTGACAGTAATAGT18100.17262888033480467No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACT17940.1711028791826738No Hit
GACTATTACTGTCAGGTGTGGGACAGCAGCACCGAGCATGTCTTCGCAACTGGGACCAAGGTCACCGTCCTAGGT17900.17072137889464106No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17680.1686231273104611No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17500.16690637601431388No Hit
CCCCTGTGCTGGTCATCTATAGGGATAGCAACCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAACTCGG17130.16337749835001125No Hit
GGTATCAACAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCTATAGGGATAGCAACCGGCCCTCTGGGATCCCT16790.16013474590173316No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16620.1585133696775941No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTC16570.1580364943175532No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC16280.155270617229316No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC16000.152600115213087No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA15790.1505972387009152No Hit
GGGTGGCCGTGTTCCCCGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCAGAGGGCCGGTTGCTATCCCTATA15730.15002498826886615No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTGCGAAGACATGCTCGGT15460.1474498613246453No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAGGATTACCTGTGGGGGACACAACATTGGAAGTAAAAATGT15400.1468776108925962No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15180.14477935930841626No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTAATGCTCGGGATACCCG14920.14229960743620362No Hit
GTGCTGCTGTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGGCC14870.14182273207616272No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTGCGAAGACATGCTCGGTGCTGCTGTCCCACACCT14800.14115510657210548No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTCCAAGGACGTCATGCAGGGCACAGAC14300.1363863529716965No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT13900.1325713500913693No Hit
GCACAGGGGCCTGGCCTGGCTTCTGTTGATACCAGTGCACATTTTTACTT13710.1307592237232139No Hit
GTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGGCCGTGTTCCCCGAGTTGGAGCCAGA13700.13066384865120573No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13640.13009159821915664No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTAATGCTCGGGATACCCGGCTTT13510.12885172228305034No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTGCGAAGACATGCTCGGTGCTGC13350.12732572113091947No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13210.12599047012280495No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACACACATGGAGGACGCATTCTGCTGGAA12950.12351071825059227No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGG12930.12331996810657593No Hit
ATATACGGCCGTGTCCTCGACTCTCAGGCCGTTCATTTGCAAATACAGTGTGTTCTTGGAATTGTCTCTGGAGAT12880.12284309274653502No Hit
GTATGTGTCACCACCACTACCACTAATAGTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCT12800.12208009217046958No Hit
CTATTACTGTCAGGTGTGGGACAGCAGCACCGAGCATGTCTTCGCAACTGGGACCAAGGTCACCGTCCTAGGTCA12520.11940959015424056No Hit
GACACATACTATGCAGACTCCATGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACACTGTATTT12300.11731133857006061No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT12290.11721596349805244No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCT12240.11673908813801155No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGGA12050.11492696176985615No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC12050.11492696176985615No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT12030.11473621162583977No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC11990.11435471133780706No Hit
GCCGTATATTGCTGTGCGAAAGCCGGGTATCCCGAGCATTACAACTGGTTCGACCCCTGGGGCCAGGGAACCCTG11990.11435471133780706No Hit
GTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGGCCGTGTTCCC11820.11273333511366802No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC11580.1104443333854717No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG11540.11006283309743899No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTG11320.10796458151325905No Hit
GTGTGGGACAGCAGCACCGAGCATGTCTTCGCAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAAC11290.1076784562972345No Hit
GAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGA11130.10615245514510364No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGT11080.10567557978506273No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTG11080.10567557978506273No Hit
GTTGATACCAGTGCACATTTTTACTTCCAATGTTGTGTCCCCCACAGGTAATCCTGGCCGTCTGTCCCAGGGCCA11050.10538945456903821No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGATTGCTGAGCTGCCTCCCAAAGTGAGC10990.10481720413698913No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10990.10481720413698913No Hit
GCATAGTATGTGTCACCACCACTACCACTAATAGTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC10980.10472182906498095No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATAGCTGCTAAAGGTGAATCCAGAGGCTGCACA10920.10414957863293187No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT10910.1040542035609237No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT10900.10395882848891552No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC10690.10195595197674374No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGA10680.10186057690473556No Hit
GTATATTGCTGTGCGAAAGCCGGGTATCCCGAGCATTACAACTGGTTCGA10640.10147907661670284No Hit
CTCCATGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACACTGTATTTGCAAATGAACGGCCTGA10610.1011929514006783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)