FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272260
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC60210.47325232263845435No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAATTACCACTGCTGGCTGCTGAT55670.4375677927467656No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG52850.4154025120651439No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAATTACCA48620.3821545910427114No Hit
GTCTTTATATATCACCACCACAGGGGCCTGGCCTGGCTTCTGCTGGTACC45720.3593605080722494No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39260.30858472324839265No Hit
CTCTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG38290.30096049549620363No Hit
ATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGCGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACGT38010.29875968748526244No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC35870.2819392262587836No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG35700.2806030213949979No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG35290.2773804096646912No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGCCAAAG34520.271328187634603No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGGTCAGCTCCATGTAGG33490.2632323581657837No Hit
GACTATTACTGTCAATCAGCAGCCAGCAGTGGTAATTATGTGGTATTCGG30350.2385518683288007No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30150.23697986260669987No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC29060.2284124314212504No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA29040.2282552308490403No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT27670.2174869916526496No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGA27430.21560058478612862No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25670.20176693443164131No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25540.20074513071227576No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24140.18974109065757No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC23820.18722588150220867No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG23560.1851822740634776No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23360.18361026834137678No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23210.18243126404980115No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23150.1819596623331709No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22870.17975885432222974No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAATTACCAC22540.17716504488076337No Hit
CTATTACTGTCAATCAGCAGCCAGCAGTGGTAATTATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGG22360.17575023973087261No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGCCAAAGCAATATGCTTATTGG21630.1700124188452046No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21070.16561080282332227No Hit
GTGTATTACTGTGCGAGGGGTCCCGACTTTGGAGTGGCGAAACAATACTA20630.16215239023470046No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT20310.15963718107933914No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC20270.15932277993491897No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20040.157514973354503No Hit
GGCCAGGCCCCTGTGGTGGTGATATATAAAGACAGTGAGAGGCCCTCAGG20030.15743637306839797No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTGCAGAGCAGGTGA19940.1567289704934526No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19870.15617876849071732No Hit
TCCTCACTCTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCA19640.15437096191030136No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGGTCAGCTCCATGTA19040.14965494474399887No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18930.14879034159684343No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18750.14737553644695267No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTG18620.14635373272758714No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGT18430.14486032729159132No Hit
GCCTACATGGAGCTGACCAGCCTGAGATCTGAGGACACGGCCGTGTATTA18400.14462452643327622No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG18150.14265951928065018No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG17980.14132331441686447No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17560.13802210240045273No Hit
GTATTACTGTGCGAGGGGTCCCGACTTTGGAGTGGCGAAACAATACTACTACTTGGACGTCTGGGGCAAAGGGAC17130.13464229009793596No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17080.13424928866741073No Hit
GCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGGTGATATATAAAGACAGTGAGAGGCC16850.1324414820869948No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG16740.13157687893983933No Hit
CCCCTGTGGTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCT16680.13110527722320908No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT16470.12945467121500323No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16340.12843286749563768No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTGCAGAGCAGGTGATCCTGGC16330.12835426720953264No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16170.127096662631852No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15990.12568185748196123No Hit
GTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGCCAGCAG15950.12536745633754107No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC15810.12426705233207049No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15380.12088724002955371No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGCCAGCAGTGGTAATTATGTGGTAT15250.11986543631018816No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15170.11923663402134783No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTGTCTTTGGC15100.11868643201861254No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG15060.11837203087419239No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14890.11703582601040668No Hit
CCATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAAGGGTGACTCTGCCCTGGAAATTCTGTGCGTAGTT14860.11680002515209156No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA14520.11412761542452016No Hit
GCTTGGTCCCTCCGCCGAATACCACATAATTACCACTGCTGGCTGCTGATTGACAGTAATAGTCAGCCTCGTCT14320.11255560970241932No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14220.1117696068413689No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14200.11161240626915883No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14180.11145520569694872No Hit
GGGATCCCTGAGGGCCTCTCA14150.11121940483863361No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCG13960.10972599940263783No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13820.10862559539716725No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13760.10815399368053699No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13690.10760379167780172No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAATTAC13530.10634618710012106No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13500.10611038624180591No Hit
GTCCTGGGGACACTGACACCGAGGGTGGCTGTGTCAGCTCATAGGAGGCC13440.10563878452517568No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG13420.10548158395296559No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13350.10493138195023029No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCT13320.10469558109191518No Hit
GCATATTGCTTTGGCAATGCATCTGCAGAGCAGGTGATCCTGGCCGTCTGTCCTGGGGACACTGACACCGAGGG13020.10233757250876392No Hit
ACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCGCAGGGATCCCTGAGGGCCTCTCA13000.10218037193655384No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12960.10186597079213369No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12960.10186597079213369No Hit
CAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGCCAGCAG12860.10107996793108327No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12800.10060836621445303No Hit
CCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCGCAGGGATCCCTGAGGGCCTCTCA12740.10013676449782277No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12740.10013676449782277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)