FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272260
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC59800.4700297109081477No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC52540.41296590319588766No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT48800.3835693961926021No Hit
GTCTTTATATATCACCACCACAGGGGCCTGGCCTGGCTTCTGCTGGTACC45560.35810290349456875No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39910.3136937418452203No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAATTACCA39670.3118073349786993No Hit
ATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGCGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACGTT38430.3020608995016742No Hit
CTCTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACG36920.29019225629981293No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33920.2666121704683005No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGCCAAAG33020.25953814471884673No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC32970.25914514328832156No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG32250.2534859226887586No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGGTCAGCTCCATGTAGG29300.23029883828777137No Hit
GACTATTACTGTCAATCAGCAGCCAGCAGTGGTAATTATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA28030.22031660195243108No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC27440.21567918507223366No Hit
GTGTATTACTGTGCGAGGGGTCCCGACTTTGGAGTGGCGAAACAATACTACTACTTGGACGTCTGGGGCAAAGG27420.21552198450002358No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG27200.21379277820571266No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGAT26950.21182777105308664No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24820.1950859101127128No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24760.19461430839608257No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24740.19445710782387246No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24710.19422130696555737No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA24000.1886406866520994No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCA23640.18581107635231792No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23210.18243126404980115No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG22570.1774008457390785No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGC22400.17606464087529278No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA22360.17575023973087261No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG21880.17197742599783064No Hit
CTATTACTGTCAATCAGCAGCCAGCAGTGGTAATTATGTGGTATTCGGCG21300.16741860940373823No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAATTACCACTGCTGGCTGCTGATTGACAGTAATA21280.16726140883152815No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG21240.16694700768710796No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20640.1622309905208055No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT20430.16058038451259965No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGA20100.15798657507113326No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG19830.15586436734629713No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTGCAGAGCAGGT19350.15209155361325516No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT19300.15169855218272996No Hit
TCCTCACTCTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCA19270.15146275132441483No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18870.14831873988021316No Hit
GGCCAGGCCCCTGTGGTGGTGATATATAAAGACAGTGAGAGGCCCTCAGG18790.14768993759137283No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT18520.1455677298665367No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA18240.14336692185559555No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17820.14006570983918382No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTGCA17760.13959410812255355No Hit
GCCTACATGGAGCTGACCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGGGGTCCCGACTTTGGA17570.13810070268655777No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17400.13676449782277209No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG17320.13613569553393173No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT16830.13228428151478472No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC16660.130948076650999No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16640.13079087607878892No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGGTCAGCTCCATGTAGGCTGTGCTCGTGGATTCGTCCGCG16610.1305550752204738No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT16540.13000487321773851No Hit
CCCCTGTGGTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGCGCGATTCTCTGGCTCCAGCTCAG16350.12851146778174272No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTGCAGAGCAGGTGATCCTGGC16280.12796126577900743No Hit
GTATTACTGTGCGAGGGGTCCCGACTTTGGAGTGGCGAAACAATACTACTACTTGGACGTCTGGGGCAAAGGGAC15980.1256032571958562No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG15770.12395265118765034No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15310.12033703802681842No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15280.1201012371685033No Hit
GCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGGTGATATATAAAGACAGTGAGAGGCC15250.11986543631018816No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCA15180.11931523430745287No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15170.11923663402134783No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG15150.11907943344913775No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA14870.1168786254381966No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC14850.1167214248659865No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14610.11483501799946552No Hit
GGGATCCCTGAGGGCCTCTCA14410.11326301227736468No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14140.11114080455252857No Hit
CCATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAAGGGTGACTCTGCCCTGGAAATTCTGTGCGTAGTTT13960.10972599940263783No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCA13950.10964739911653279No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13900.10925439768600757No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG13680.10752519139169665No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTGTCTTTGGC13630.10713218996117146No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC13580.10673918853064625No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAG13570.10666058824454121No Hit
GTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGCCAGCAGTGGTAATTATGTGGTATTCGGCGGA13540.10642478738622609No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAATTAC13480.10595318566959584No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13460.10579598509738576No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13340.10485278166412525No Hit
GCTTGGTCCCTCCGCCGAATACCACATAATTACCACTGCTGGCTGCTGATTGACAGTAATAGTCAGCCTCGTCTT13100.10296637479760426No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT13090.10288777451149922No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13040.10249477308097402No Hit
CAGGGATCCCTGCGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACGTTGACCATCAGTGGAGTCCAGGCA13000.10218037193655384No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12810.10068696650055806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)