FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105721
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTACCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA106360.9619063036697323No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC95970.8679404659945864No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50020.4523745140048891No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT49890.45119881054985844No Hit
GTATTAATATCTTGTTGGCCTGGTATCAGCAGAAACCAGGGAGAGCCCCTAAGTTCCTGATCTATAGGGCGTCTA33620.3040550012164009No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT33430.30233666539750986No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33210.3003470133966887No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29480.2666133681100386No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29100.26317669647225655No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28660.2591973924706142No Hit
GATCTATAGGGCGTCTACTTTAGAAACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA28050.25368063010470093No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26700.24147140191784366No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26450.23921043373509232No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC26280.2376729753708214No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26200.23694946555234098No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25350.22926217373098637No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25110.22709164427554507No Hit
CCTTCCACCCTGTCTGCGTCTCTAGGAGACAGAGTCATTATTACTTGCCGGGCCAGTCAGAGTATTAATATCTTG23320.21090311208704546No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22390.20249231044721047No Hit
CAGTATAATAGTTACCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22020.1991460775367385No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT20640.18666553316795106No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20320.18377149389402933No Hit
GAGTATTAATATCTTGTTGGCCTGGTATCAGCAGAAACCAGGGAGAGCCCCTAAGTTCCTGATCTATAGGGCG20040.1812392095293478No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTAC19760.17870692516466632No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19650.17771209916425573No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19620.17744078298232557No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACA19210.17373279516261333No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC19080.17255709170758265No Hit
GTGTAAGGGTAACTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATC19010.1719240206164123No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18900.17092919461600167No Hit
ATATTAATACTCTGACTGGCCCGGCAAGTAATAATGACTCTGTCTCCTAGAGACGCAGACAGGGTGGAAGGAGA18640.16857778770594029No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATG17610.15926259879300475No Hit
CCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG17380.15718250806487352No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16770.1516657456989602No Hit
GGGTAAAACCCTGGGGAGTGGGAGCTACTGCTCCATATTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTC16410.14840995151579828No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC16310.14750556424269776No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15940.14415933133222575No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15930.1440688926049157No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15750.14244099551333475No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15670.14171748569485432No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15580.14090353714906384No Hit
CTAGGAGACAGAGTCATTATTACTTGCCGGGCCAGTCAGAGTATTAATATCTTGTTGGCCTGGTATCAGCAGAAA15540.1405417822398236No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG15540.1405417822398236No Hit
CTATAGATCAGGAACTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACAAGATATTAATACTCTGAC15480.1399991498759633No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15290.13828081405707227No Hit
TCTATAGGGCGTCTACTTTAGAAACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCAC15200.1374668655112818No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG14910.13484414241929024No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14720.1331258066003992No Hit
GTCTGCGTCTCTAGGAGACAGAGTCATTATTACTTGCCGGGCCAGTCAGAGTATTAATATCTTGTTGGCCTGGTA14450.13068396096302776No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14190.12833255405296634No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC14070.1272472893252457No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13920.1258907084155949No Hit
CATTATTACTTGCCGGGCCAGTCAGAGTATTAATATCTTGTTGGCCTGGTATCAGCAGAAACCAGGGAGAGCCC13890.12561939223366472No Hit
GTCTCCTTCCACCCTGTCTGCGTCTCTAGGAGACAGAGTCATTATTACTTGCCGGGCCAGTCAGAGTATTAATA13660.1235393015055335No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13520.12227315932319274No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT13300.12028350732237154No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT12970.11729902932113977No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT12930.11693727441189958No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12700.11485718368376832No Hit
CCCTATAGATCAGGAACTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACAAGATATTAATACTCTGA12640.11431455131990802No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12620.1141336738652879No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCAGTTTCTAAAGTAGACGCCCTATAGA12600.1139527964106678No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12460.11268665422832704No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12420.11232489931908682No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA12340.1116013895006064No Hit
GGTATCAGCAGAAACCAGGGAGAGCCCCTAAGTTCCTGATCTATAGGGCGTCTACTTTAGAAACTGGGGTCCCA12180.11015436986364553No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12150.10988305368171537No Hit
GGCCAAAAGTGTAAGGGTAACTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGA12100.10943086004516511No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG12070.10915954386323493No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11960.10816471786282435No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11810.10680813695317354No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11570.10463760749773224No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11540.10436629131580209No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG11540.10436629131580209No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG11490.10391409767925182No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11340.10255751676960102No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGA11300.1021957618603608No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11270.10192444567843065No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11220.10147225204188036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGG358.710876E-488.6402970
GTTATTA951.891749E-976.19955470
TGTTAGA12950.051.90648370
CGTATGC1500.050.2673441
TCGTATG1600.047.08019340
TTCTGTC28050.045.32463569
CAGTTCG16000.043.30447470
ACGTAGA1950.041.59547827
GAGACGT1950.041.54840524
CTTATAC2050.041.1479031
GTGGTAT2250.040.4895321
CGTAGAG2050.039.58796328
AGACGTA2050.039.53418425
CGAGACG2050.039.51986723
ATTCTGT25450.039.4514768
GTGGCCG11800.039.4374270
CACGAGA2100.038.56669621
AATGGTA2701.2369128E-1038.3013670
ACGTTCG1100.001699374437.60497370
TATTCTA1657.4226737E-637.60497370
Produced by FastQC (version 0.11.5)