FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004685730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849004
Sequences flagged as poor quality0
Sequence length19-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC29500.34746597189176964No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG26040.30671233586649765No Hit
GTATTATTGTGCGAGAGGGGCAGTTACTGGATCTGATGTTTTTGATATCTGGGGCCAAGGGACAATGGTCACCG20650.2432261803242388No Hit
CAATAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGCCACAGTGAATTCTTGACATTGTCTC19620.2310943175768312No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTTCTCGATGGGGGCTGGGAGGCCTTTG19530.23003425189987328No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19410.22862083099726266No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG19150.22555841904160642No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19110.2250872787407362No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCACAAAATTGTTGCTGCCTGCATATGAA18830.2217892966346448No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCC18580.2188446697542061No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCT18440.21719567870116044No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGAAGCGGTTGTGCAG16430.19352087858243305No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGACAG15670.18456921286589933No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC15440.1818601561358957No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCACAAAATTGTTG15130.17820881880415168No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14890.17538197699893052No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT14810.17443969639719012No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA14410.16972829338848816No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCGTCTTC14280.16819708741066No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT13920.16395682470282824No Hit
CTCCTGTGCAGCCTCTGGATTCGTCTTCAATGACTACTACATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGG13650.16077662767195441No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13520.1592454216941263No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTATAAAAGGTGTCCA13470.15865649631803855No Hit
ATAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGCCACA13360.1573608604906455No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC13170.15512294406151209No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12780.15052932612802766No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12700.1495870455262873No Hit
GTGTTGTAGTTGTTCTCCGGCTGCCCGCTGCTCTCCCACTCCACGGCGAT12590.14829140969889423No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGGGGAAGACCGATGGGCCCTTGG12490.14711355894671874No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGT12210.14381557684062737No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12140.14299108131410454No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11860.13969309920801315No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11690.13769075292931482No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT11650.13721961262844462No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGACTGAGCTGGGTTTTC11370.13392163052235326No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA11360.1338038454471357No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11340.1335682752967006No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATT11160.1314481439427847No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTATTGCAGTTCATATGCAGGCAGCAACAATTTTGTGGTAT11050.1301525081153917No Hit
GACCTACACCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCTCAAAACCCCACT10940.12885687228799864No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC10800.12720788123495294No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTAATTATAACTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC10800.12720788123495294No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG10740.12650117078364767No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10600.12485217973060199No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTA10530.12402768420407913No Hit
GGACAATGGTCACCGTCTCTTCAGCTTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCA10520.12390989912886158No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10450.12308540360233874No Hit
GGATGAGGCTGATTATTATTGCAGTTCATATGCAGGCAGCAACAATTTTGTGGTATTCGGCGGAGGGACCAAA10430.12284983345190363No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10430.12284983345190363No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10390.12237869315103345No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC10340.1217897677749457No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10340.1217897677749457No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGTGA10300.12131862747407551No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10110.11908071104494208No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10050.11837400059363677No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTTCAACAGCACGTTCCGTG10050.11837400059363677No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10010.11790286029276659No Hit
GTGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT9970.11743171999189637No Hit
GAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGT9770.11507601848754542No Hit
CTACAACACCACGCCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG9700.11425152296102256No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC9490.11177803638145405No Hit
CCATTGTCCCTTGGCCCCAGATATCAAAAACATCAGATCCAGTAACTGCCCCTCTCGCACAATAATACACGGCCG9470.11154246623101893No Hit
GTGTATTATTGTGCGAGAGGGGCAGTTACTGGATCTGATGTTTTTGATAT9330.10989347517797324No Hit
CCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAAC9250.10895119457623285No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG9190.10824448412492757No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG9170.10800891397449246No Hit
GGGGAGGTGTGTCACAAGATTTGGGCTCTGGGCACCGTGGGCATGTGTGA9150.10777334382405736No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC9090.10706663337275207No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9000.10600656769579413No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8790.1035330811162256No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTAATTATAACTTTGTCTCCTGGTACCAACAGCA8790.1035330811162256No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCT8790.1035330811162256No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8770.1032975109657905No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT8730.10282637066492031No Hit
CTACTACATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATTTCATACATTAGTGGTAGCACTAT8630.10164851991274482No Hit
GTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTT8580.10105959453665708No Hit
GTTTGGTCCCTCCGCCGAATACCACAAAATTGTTGCTGCCTGCATATGAACTGCAATAATAATCAGCCTCATCC8570.10094180946143953No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)