FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849004
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42150.49646409204196923No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT23840.2807996193186369No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTC22680.2671365505934012No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT22450.2644274938633976No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19100.22496949366551866No Hit
GTATTATTGTGCGAGAGGGGCAGTTACTGGATCTGATGTTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGT18880.22237822201073257No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18630.21943359513029387No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCACAAAATTGTTGCTGCCTGCATATGAA18050.21260206076767602No Hit
CAATAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGCCACAGTGAATTCTTGACATTGTCTCTG17950.21142421001550052No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCT17750.20906850851114953No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17300.20376818012635983No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGG16660.19622993531243668No Hit
GTGTTGTAGTTGTTCTCCGGCTGCCCGCTGCTCTCCCACTCCACGGCGAT16410.19328530843199795No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGAAGCGGTTGTGCAGAGCCTCATGCATCACGGAGCATGA15970.1881027651224258No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC14810.17443969639719012No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCACAAAATTGTTG14730.17349741579544975No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGACA14710.17326184564501462No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14150.1666658814328319No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA13980.16466353515413357No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13980.16466353515413357No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC13700.16136555304804218No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA13370.15747864556586305No Hit
CTCCTGTGCAGCCTCTGGATTCGTCTTCAATGACTACTACATGAGCTGGATCCGCCAGGCTCCAGGGAAGGG13300.15665415003934022No Hit
ATAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGCCACAGTGAATTCTTGACATTGTCTCTGGA12760.15029375597759256No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12630.14876254999976443No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTATAAAAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCG12120.14275551116366944No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12100.14251994101323434No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA12050.14193101563714658No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCGTCTTCAATGACTACTACATGAGCTGGATCC11980.14110652011062375No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATT11720.13804410815496745No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11610.13674847232757445No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG11340.1335682752967006No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGACTGAGCTGGGTTTTC11250.13250820961974266No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTT11110.13085921856669697No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG11030.1299169379649566No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG10870.1280323767614758No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTATTGCAGTTCATAT10820.12744345138538804No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG10720.12626560063321257No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA10640.12532332003147217No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTT10530.12402768420407913No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGT10480.1234387588279914No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10170.11978742149624737No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTAATTATAACTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC10160.11966963642102982No Hit
GGACAATGGTCACCGTCTCTTCAGCTTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCA10150.11955185134581225No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA10030.11813843044320169No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG9980.11754950506711392No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT9930.11696057969102619No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT9820.11566494386363316No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA9770.11507601848754542No Hit
GAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGT9750.11484044833711031No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG9690.114133737885805No Hit
GGGGAGGTGTGTCACAAGATTTGGGCTCTGGGCACCGTGGGCATGTGTGAGTTGTGTCACCAAGTGGGGTTTTGA9660.11378038266015238No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGC9600.11307367220884706No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTTCAACAGCACGTTCCGTGT9450.11130689608058383No Hit
GGATGAGGCTGATTATTATTGCAGTTCATATGCAGGCAGCAACAATTTTGTGGTATTCGGCGGAGGGACCAAA9380.110482400554061No Hit
GTGTATTATTGTGCGAGAGGGGCAGTTACTGGATCTGATGTTTTTGATATCTGGGGCCAAGGGACAATGGTCACC9310.10965790502753817No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC9120.10741998859840472No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9120.10741998859840472No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG9070.10683106322231697No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTACACGGGGTACGTGCCAAGCATCCTC9070.10683106322231697No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG9050.10659549307188186No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT9030.10635992292144679No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9010.10612435277101168No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG8880.10459314679318353No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG8870.10447536171796598No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT8800.10365086619144315No Hit
GTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGATGTTCCCCTGCTGCCACCTGCTCTTGTCCACGGTGA8770.1032975109657905No Hit
CTACAACACCACGCCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGA8700.10247301543926765No Hit
GTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTT8690.1023552303640501No Hit
GTGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT8610.10141294976230972No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATC8600.10129516468709217No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8530.10047066916056932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGTA150.0029780986193.7510470
CCTTTGT9400.071.11075670
CCCACTT4050.064.5836970
TATTCTA951.5403607E-461.1845470
CTTCAGT800.005992379554.4924870
AATGGTA1103.178651E-452.84119470
CACGATT200.007172500752.08584253
TACGCGT200.00744455651.59775544
TAGAGCA200.00784956650.9114
GCCGTTA352.5003343E-548.49523515
TGACAGT13100.044.37047270
GTATCAA14200.040.2025071
GCCGGCA3301.3260433E-939.63089870
GGTATCA6000.039.0777931
TGGTATC2200.038.5757562
TTAACGG451.0689075E-437.91055735
TCCAGTT9650.037.64592770
GGTGGAA10950.037.15773870
GGCGTCA16050.035.30977270
CACTATT8400.034.59840470
Produced by FastQC (version 0.11.5)