FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1465896
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAACAGTATGGTAGCTCACCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTG50200.34245267058508927No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT48970.3340618979791199No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT43740.29838405998788453No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38600.2633201809678176No Hit
GTGTATTACTGTCAACAGTATGGTAGCTCACCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA29550.20158319553365311No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT28880.19701261208162107No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT28870.19694439441815792No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28750.19612578245659992No Hit
GTAATATAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA28020.19114589302378887No Hit
GTATGGTAGCTCACCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCA27800.18964510442759924No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27630.18848540414872542No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26540.1810496788312404No Hit
CAGTAATATAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGGGAGTTCTTGGCGTTGTCTC25570.1744325654753134No Hit
GCCTTATATTACTGTGCGAGACGGGGGTACTATGGTTCAGGGAGTGATGC25410.17334108285990274No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTCCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA25230.1721131649175658No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24940.17013485267713396No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTCCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24930.17006663501367084No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG24780.16904337006172335No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23930.1632448686673543No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23860.16276734502311216No Hit
CTCCTGCAGGGCCAGTCAGACTATTAGTAGCAGCTACTTAGGCTGGTACC23840.16263090969618582No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23760.1620851683884805No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC23450.15997042082112237No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22880.15608201400372196No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22740.15512696671523765No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGTAGCAGCTACTTAGGC22530.15369439578251118No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22100.15076103625359508No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22100.15076103625359508No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA21810.14878272401316328No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC21510.14673619410926833No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21160.14434857588805752No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC21140.1442121405611312No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGGAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAACAGTA21010.14332531093611006No Hit
GTATGGTACGGCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGGCTCTGGA20780.14175630467645725No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20660.14093769271489928No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGTAG20450.1395051217821728No Hit
ATATTACTGTGCGAGACGGGGGTACTATGGTTCAGGGAGTGATGCCTACT20190.13773146253213053No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG19930.13595780328208823No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGTACCATAGATGAGGAGCC19920.13588958561862505No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC19720.13452523234936176No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19490.13295622608970895No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT19420.13247870244546678No Hit
ATATAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGGGAGTTCTTGGCGTTGTCTCTGGAGA19270.13145543749351932No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19140.1305686078684982No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18910.12899960160884538No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG18730.1277716836665084No Hit
GCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTC18380.12538406544529762No Hit
CTCCTGTGTAGGCTCTGGATTCACCTTTGATGATTATGGCATGAGCTGGGTCCGCCAAGCTCCAGGGAAGGGG18280.12470188881066598No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT18100.123473970868329No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG18010.12286001189716052No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC17550.11972199937785491No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC17500.11938091106053908No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC17450.11903982274322326No Hit
ACCATACTGTTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTCC17270.11781190480088628No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17190.11726616349318096No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCACTGTGAGGTGCAGGTGGTGGAG17170.11712972816625464No Hit
GACTATTAGTAGCAGCTACTTAGGCTGGTACCAGCAGAAACCTGGCCAGG16970.11576537489699132No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG16530.11276379770461206No Hit
CTACCATACTGTTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTCCTGATGGTGAGAGTGAAGTCTGTC16350.1115358797622751No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT16300.11119479144495925No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGTACCATAGA16280.11105835611803293No Hit
GTTATGGAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCC16240.11078548546418028No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC16000.1091482615410643No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15800.10778390827180101No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA15740.10737460229102201No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC15610.10648777266600085No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTG15390.10498698406981122No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15320.10450946042556908No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCACGTGTTTCCATTCAGTGA15220.10382728379093743No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15140.10328154248323208No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC14870.10143966556972664No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14780.10082570659855816No Hit
GTCTGAGAGCCGAGGACACGGCCTTATATTACTGTGCGAGACGGGGGTACTATGGTTCAGGGAGTGATGCCTACT14720.10041640061777916No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14660.10000709463700017No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAAAT200.004423393458.855367
CTCTTAT2900.049.6795461
GTCGTAT306.668855E-445.48941439
TGTTAGA12050.045.45476570
GGTATCA11200.044.8725131
AATCTCG7750.043.80288336
ATCTCGT7900.043.04557837
CTAATCT8050.042.90265334
GCTAATC8100.042.5805733
TCGTATG9200.042.35494240
CGCTAAT9150.042.06950832
GCCGTCT9400.042.00050446
TCGCTAA9250.041.9177731
TAATCTC8100.041.85229535
CGTATGC9400.041.55646541
CCGTCTT9600.041.24184447
ACTCGCT9550.041.213729
CTTATAC9600.041.1831
CTCGCTA9600.041.04262530
GTATGCC9800.040.9936342
Produced by FastQC (version 0.11.5)