FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1465896
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98450.6716028967948613No Hit
GTATTACTGTCAACAGTATGGTAGCTCACCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGT43060.29374525887238934No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42410.28931111074728355No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA37770.25765811490037493No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT29580.20178784852404263No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28650.1954436058219683No Hit
GTGTATTACTGTCAACAGTATGGTAGCTCACCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA28440.19401103488924182No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27250.18589313293712514No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC25970.17716127201384No Hit
GTATGGTAGCTCACCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT24690.16842941109055487No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24110.16447278660969128No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG23400.15962933250380654No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG23350.1592882441864907No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC23290.15887893820571172No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23250.15860606755185908No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTCCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23080.15744636727298528No Hit
GTAATATAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGGGAGTTCTTGGCGTTGTCTCTGGA22990.15683240830181677No Hit
GCCTTATATTACTGTGCGAGACGGGGGTACTATGGTTCAGGGAGTGATGC22950.15655953764796413No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC22930.1564231023210378No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTCCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA22820.15567270802294295No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22580.154035484099827No Hit
CAGTAATATAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGGGAGTTCTTGGCGTTGTCTCTG22570.15396726643636383No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC22490.1534215251286585No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG22380.1526711308305637No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGTAGCAGCTACTTAGGCTG22040.1503517302728161No Hit
CTCCTGCAGGGCCAGTCAGACTATTAGTAGCAGCTACTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAG21840.14898737700355277No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA21230.1448260995322997No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA20780.14175630467645725No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGGAGACTGGAGCCTGAAGATT20270.13827720383983585No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20120.13725393888788837No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19740.1346616676762881No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19210.13104613151274033No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGTA18970.12940890758962437No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18940.12920425459923487No Hit
GTATGGTACGGCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGGCTCTGGATTCACCTTTGATGATTATGGCATGA18750.12790811899343474No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT18740.12783990132997156No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGTACCATAGATGAGGAGCCTG18540.12647554806070826No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGTAGCA18430.12572515376261345No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18410.1255887184356871No Hit
CTCCTGTGTAGGCTCTGGATTCACCTTTGATGATTATGGCATGAGCTGGGTCCGCCAAGCTCCAGGGAAGGG18260.12456545348373962No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG18170.12395149451257116No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18000.12279179423369735No Hit
ATATTACTGTGCGAGACGGGGGTACTATGGTTCAGGGAGTGATGCCTACTTTGACTACTGGGGCCAGGGAACCCA17760.12115457031058138No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT17580.1199266523682444No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC17220.11747081648357045No Hit
GCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTC17030.11617468087777032No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC16840.11487854527197018No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16610.11330953901231737No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC16600.11324132134885423No Hit
ATATAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGGGAGTTCTTGGCGTTGTCTCTGGAGAT16480.11242270938729623No Hit
GACTATTAGTAGCAGCTACTTAGGCTGGTACCAGCAGAAACCTGGCCAGG16370.11167231508920142No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA16360.11160409742573825No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA16340.11146766209881193No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16320.1113312267718856No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT16270.11099013845456977No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGTACCATA16150.11017152649301179No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15860.10819321425257998No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15810.10785212593526416No Hit
ACCATACTGTTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTCC15630.1066242079929272No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCACTGTGAGGTGCAGGTGGTGGAGT15580.10628311967561138No Hit
CTACCATACTGTTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTCCTGATGGTGAGAGTGAAGTCTGTC15410.10512341939673757No Hit
GTCTGAGAGCCGAGGACACGGCCTTATATTACTGTGCGAGACGGGGGTAC15290.10430480743517957No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC15140.10328154248323208No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCACGTGTTTCCATTCAGT15030.10253114818513727No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14740.10055283594470549No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC14670.10007531230046335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA3700.088.2253970
CGAGCTT600.001209379481.6084970
CCGCTAT750.002931001165.2867970
TTCTTAA1653.200039E-759.3516370
GATGAAT1202.7544296E-454.4056670
GAGCAAA21300.045.97661670
GAGCCAT20750.045.62209370
GGTATCA10550.042.8080331
AATTCAT1600.001137316340.80424570
ACTTCCA3751.4915713E-939.17207370
AAGCGGT18000.038.99072370
GCGCTTT702.8929207E-838.2027813
AAGGACA23300.037.8271170
TGAGCTA16050.036.60941770
GTATCAA24650.036.100121
TTTAAGT1850.002320679835.29015770
GACAGCC2600.033.4536861
AAATGAA15450.032.7490470
ATGACTT3052.1896494E-532.10825770
AGACACA21950.031.97416770
Produced by FastQC (version 0.11.5)