FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962371
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCCTCGGCTCTAAGGGGATTCATGTGCAGATACA38990.40514520907217694No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT34930.3629577366732788No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT29170.3031055590827238No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29170.3031055590827238No Hit
CTTTTATACACCTCCAACAAAAAGAACTACCTAGCTTGGTACCAGCAAAA27150.2821157329138139No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGGGGGCTGGGAGGGCTTTG25840.26850351891318425No Hit
ATATATTACTGTGCGAAAGACGGAGTGGGCGGCCGTGGCTGGTACGGAGG25790.2679839687604884No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23140.24044781066761156No Hit
CTCCTAAACTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGAGCGATTTAGTGGCAGCGGGTC21890.2274590568502168No Hit
GGACTGAACAGAGAGAACTCATCATGGAGTTTGGGCTGAGCTGGCTTTTT21830.22683559666698186No Hit
ATATTATGGTACTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA21320.2215361851094848No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT21210.22039317477355408No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21080.21904234437654502No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19790.20563795043699365No Hit
GTTTAGGAGGCTGTCCTGCTTTTTGCTGGTACCAAGCTAGGTAGTTCTTTTTGTTGGAGGTGTATAAAAGAC19760.20532622034537618No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19460.20220891942920144No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCT19120.19867597839087003No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18270.1898436257950416No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17570.18256992365730057No Hit
CCTTTAACACCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGAGGCTAGAGTGGGTCTCAACTATTAG17240.17914089264950836No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17070.17737442213034266No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16740.17394539112255045No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16520.17165937045068896No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC16330.16968507987044498No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG16250.1688537996261317No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16020.16646386892373108No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15360.15960580690814666No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15290.15887843669437254No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15200.15794324641952012No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15170.15763151632790265No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15150.15742369626682434No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTGCGGGGACTTCGTGATGACCCAG14850.15430639535064958No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGT14510.15077345431231823No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGATACAGCCAGGGAGTCTGGAGA14420.1498382640374658No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14330.14890307376261336No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG14130.1468248731518302No Hit
GTCTCCAGACTCCCTGGCTGTATCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA14110.1466170530907519No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13590.14121373150271568No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT13530.14059027131948074No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAACACCTATGCCATGAGCTGGG13490.1401746311973241No Hit
CCATAATATTGGTGACAGAAATAAACAGCCACATCGTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC13400.1392394409224717No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13210.1372651503422277No Hit
CAGTAATATATGGCCGTGTCCTCGGCTCTAAGGGGATTCATGTGCAGATACACCGTGTTCTTGGATTTGTCTC12840.13342047921227884No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC12720.13217355884580895No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC12410.12895234789909504No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGACGATGTGGCTGTTTATTTCTGTCACCAATATTATGGTAC12140.12614677707453778No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12120.12593895701345945No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11700.12157473573081483No Hit
GTGTATCTGCACATGAATCCCCTTAGAGCCGAGGACACGGCCATATATTACTGTGCGAAAGACGGAGTGGGCGGC11500.11949653512003168No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11420.11866525487571841No Hit
GATCAGGACTGAACAGAGAGAACTCATCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGG11400.11845743481464009No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11350.1179378846619443No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11290.11731442447870935No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC11180.11617141414277862No Hit
GTGTGAGGTGCACCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC11120.11554795395954368No Hit
ACCTAGCTTGGTACCAGCAAAAAGCAGGACAGCCTCCTAAACTACTCATT10990.11419712356253461No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA10830.11253456307390808No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA10830.11253456307390808No Hit
GAACAGAGAGAACTCATCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTG10810.11232674301282976No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10780.11201501292121228No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGGGGGCTGGGAGGGCTT10780.11201501292121228No Hit
GCTCTAAGGGGATTCATGTGCAGATACACCGTGTTCTTGGATTTGTCTCT10480.10889771200503756No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT10450.10858598191342007No Hit
ATATTACTGTGCGAAAGACGGAGTGGGCGGCCGTGGCTGGTACGGAGGGGGAGATAACTACTTTGACTACTGGGG10370.1077547016691068No Hit
ATTTAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGACGATGTGGCTGTTTA10290.10692342142479357No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCT10280.10681951139425441No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTAGTTATCTCCCCCTCCGTACCAGCCACGGCCGCCCACTCCGTCTTTCG10250.10650778130263693No Hit
GTTTATTTCTGTCACCAATATTATGGTACTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA10190.10588432111940198No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10090.10484522081401039No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10010.10401394056969712No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG9900.10287093023376638No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC9830.1021435600199923No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCG9810.10193573995891397No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCACCTGTTGGAG9790.10172791989783567No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9780.1016240098672965No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9750.10131227977567903No Hit
GCCATATATTACTGTGCGAAAGACGGAGTGGGCGGCCGTGGCTGGTACGGAGGGGGAGATAACTACTTTGACTA9750.10131227977567903No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC9740.10120836974513987No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC9660.1003770895008266No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCT9650.10027317947028744No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9650.10027317947028744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG4400.054.904269
ATCGTCG200.006857158652.6818749
GTGTCCA12150.050.5402270
AACTAAG600.00820719750.33308470
CGGATTA200.00852645749.85713214
TGTTAGA9150.049.50795470
TTCGGGG12600.043.9415870
CTCGTAT5450.043.2174339
TCGTATG5900.042.95383540
CTACTCA16550.042.57783570
CGTATGC6150.042.42138741
CTTGAAA6100.041.95210357
CCTATCT5400.041.84424234
CTTATAC6550.041.630511
GTATGCC6300.041.5160642
ACGGACT6200.041.4086527
TATCTCG5550.040.8900836
TCCTATC5600.040.86156533
ATCTCGT5700.040.5198237
ATGCCGT6500.040.4431544
Produced by FastQC (version 0.11.5)