FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962371
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62400.6483985905643458No Hit
GTAATATATGGCCGTGTCCTCGGCTCTAAGGGGATTCATGTGCAGATACACCGTGTTCTTGGATTTGTCTCTGGA34120.354541024199607No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28680.29801396758630505No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28660.2978061475252267No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA26560.2759850411120036No Hit
CTTTTATACACCTCCAACAAAAAGAACTACCTAGCTTGGTACCAGCAAAA24560.25520303500417196No Hit
ATATATTACTGTGCGAAAGACGGAGTGGGCGGCCGTGGCTGGTACGGAGGGGGAGATAACTACTTTGACTACTGG24090.2503192635688316No Hit
GGACTGAACAGAGAGAACTCATCATGGAGTTTGGGCTGAGCTGGCTTTTT21570.22413393587296374No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20750.21561331336875278No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT20150.2093787115364033No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19920.20698878083400268No Hit
ATATTATGGTACTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT19740.20511840028429787No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGG19740.20511840028429787No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAA18640.19368829692499048No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18260.18973971576450246No Hit
CTCCTAAACTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGAGCGATTTAGTGGCAGCGGGTCTG18150.18859670542857174No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC18110.1881810653064151No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTT18110.1881810653064151No Hit
GTTTAGGAGGCTGTCCTGCTTTTTGCTGGTACCAAGCTAGGTAGTTCTTTTTGTTGGAGGTGTATAAAAGACT17650.18340120390161382No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC16980.17643923185549024No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16890.17550404158063782No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA16860.17519231148902034No Hit
CCTTTAACACCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGAGGCTAGAGTGGGTCTCAACTATTAGTG16160.16791860935127928No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTC15910.16532085858780035No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15550.16158009748839067No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGA15480.16085272727461655No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGATACAGCCAGGGAGTCTGGA14960.15544940568658033No Hit
CCATAATATTGGTGACAGAAATAAACAGCCACATCGTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC14710.1528516549231014No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14660.1523321047704056No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTT14630.15202037467878812No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC14540.1510851844039357No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA14450.15014999412908328No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14320.14879916373207422No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT13920.1446427625105079No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTT13730.1426684719302639No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC13460.13986290110570662No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12810.13310874912066137No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGGGGAAGACCGATGGGCCCTTGGTGGA12720.13217355884580895No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCA12700.1319657387847306No Hit
GTCTCCAGACTCCCTGGCTGTATCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA12600.13092663847933905No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12010.12479594667752873No Hit
GTGTATCTGCACATGAATCCCCTTAGAGCCGAGGACACGGCCATATATTACTGTGCGAAAGACGGAGTGGGCGGC11950.12417248649429379No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA11890.12354902631105884No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGC11830.12292556612782388No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11730.1218864658224323No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC11720.12178255579189314No Hit
CAGTAATATATGGCCGTGTCCTCGGCTCTAAGGGGATTCATGTGCAGATACACCGTGTTCTTGGATTTGTCTCTG11670.12126300563919734No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA11640.1209512755475799No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA11560.12011999530326663No Hit
GTCCCAGACCCGCTGCCACTAAATCGCTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGTAGTTTA11560.12011999530326663No Hit
GTGTGAGGTGCACCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC11480.11928871505895336No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC11420.11866525487571841No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAACACCTATGCCATGAGCTGGG11410.11856134484517925No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT11200.11637923420385694No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10850.1127423831349864No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10670.11087200258528157No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGACGATGTGGCTGTTTATTTCTGTCACCAATATTATGGTACT10430.10837816185234177No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10370.1077547016691068No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACG10330.10733906154695019No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTAAATCGCTCAGGGACCCCG10300.10702733145533272No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA10250.10650778130263693No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10200.10598823114994115No Hit
ATTTAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC9950.10339048038646217No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGTAGTTATCTCCCCCTCCGTACCAGCCACGGCCGCCCACTCCGTCTTTC9890.10276702020322726No Hit
GAACAGAGAGAACTCATCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGT9830.1021435600199923No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9820.10203964998945314No Hit
GCTCTAAGGGGATTCATGTGCAGATACACCGTGTTCTTGGATTTGTCTCTGGAGATGGAGAACCGGCCCTTCACG9750.10131227977567903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCCG150.00263543367.0725631
GTGTCCA11800.058.4788870
CTACTCA14750.057.4156370
GAACCTT1152.7166583E-454.5494770
AATGCCA8800.046.33605670
GCTTTGT24500.044.8084970
ATCGGCT307.653628E-444.2336119
CGTGTCA307.6830835E-444.19912713
GAGCAAA14850.042.243770
GTATCAA16150.038.3994141
ACCCAGT15100.037.3898770
GGCATTA12050.036.4417670
GGGCAGT2650.036.291731
TATCAAC18050.034.517772
AGTAAAT12500.033.8752270
TGTGATT8900.033.48050370
GCTTAGG400.003151063633.163131
ATCAACG19100.032.6201973
GGTGACA14000.032.48615670
TCAACGC19450.032.0348634
Produced by FastQC (version 0.11.5)