FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1383317
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT48760.35248608959479283No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41390.2992083520986151No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35210.2545331258128108No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAATACCACAAGATTGTTTCTGCCTGCATATGA34730.251063205324593No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTAGTTACTACTGGAGCTGGATCCG32820.23725581338189294No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCT31850.2302436823952861No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28830.2084120993235824No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26940.19474928740122474No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA26630.19250829708591738No Hit
CCCCAGAGGGCCGCTGATTGACTTCAGAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGA26320.19026730677061007No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26230.18961669667906925No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCC26080.18853234652650117No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAATACCACAAGATTGTTT25420.18376120585520167No Hit
CAATAATACACGGCCGTGTCAGCGGCGGTCACAGAACTCAGCTTCAGGGAGAACTGGTTCTTGGACTTGTCTA25390.18354433582468804No Hit
ATAATACACGGCCGTGTCAGCGGCGGTCACAGAACTCAGCTTCAGGGAGAACTGGTTCTTGGACTTGTCTACT25110.1815202155398943No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT24440.17667678485842364No Hit
CTATTACTGCAGCTCATATGCAGGCAGAAACAATCTTGTGGTATTCGGCG23660.17103816406506966No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC23630.17082129403455606No Hit
GACTATTACTGCAGCTCATATGCAGGCAGAAACAATCTTGTGGTATTCGGCGGGGGGACCAAGCTGACCGTCCTA23630.17082129403455606No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23390.16908633379044716No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG23270.1682188536683927No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22930.16576099332257177No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA22570.1631585529564084No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG22280.16106214266144347No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA21980.15889344235630734No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC21950.1586765723257937No Hit
GTATTATTGTGCGAGAGATTTAGGTGGTGGTAATATCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC21890.1582428322647665No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21830.15780909220373926No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC21510.15549581187826073No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGACTATTACTGCAGCTCATAT21130.15274879149175496No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20780.1502186411357628No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG20390.14739933073908582No Hit
GTACAACCCCTCCCTCAAGAGTCGAATCACCATATCAGTAGACAAGTCCA20300.146748720647545No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG20280.1466041406272026No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG20170.14580895051531934No Hit
CCTCTGGGGTCTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAG19940.14414628028138166No Hit
GGATGAGGCTGACTATTACTGCAGCTCATATGCAGGCAGAAACAATCTTGTGGTATTCGGCGGGGGGACCAA19570.14147154990504707No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTCTGAAGTCAATCA19470.14074864980333504No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG19240.13908597956939733No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG18850.13626666917272034No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18850.13626666917272034No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT18810.13597750913203552No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18760.1356160590811795No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC18670.13496544898963866No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA18510.13380880882689938No Hit
GGACTGGAGTGGCTTGGGCGTTTCTATACCACTGGGAGCACCAAGTACAA18030.1303388883386816No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG17850.12903766815559992No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT17650.1275918679521758No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17320.12520629761652607No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17160.12404965745378681No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG17040.12318217733173235No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC16700.12072431698591139No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG16610.12007370689437057No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16320.11797729659940565No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTAGTTACTACTGGAGCTGGATCCGGCAGCCCGCCGGGAA16290.11776042656889202No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT15690.11342302595861975No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15560.1124832558263941No Hit
ATCTTGTGGTATTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGCCC15440.11161577570433966No Hit
GTGTATTATTGTGCGAGAGATTTAGGTGGTGGTAATATCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT15370.1111097456331412No Hit
GGGTTGTACTTGGTGCTCCCAGTGGTATAGAAACGCCCAAGCCACTCCAGTCCCTTCCCGGCGGGCTGCCG15090.1090856253483475No Hit
GTAATAGTCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGA14990.10836272524663544No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14900.1077121151550946No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14850.10735066510423857No Hit
GCAGTAATAGTCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGG14690.10619402494149932No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14690.10619402494149932No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14630.1057602848804721No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14380.10395303462619196No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGATATTACCACCACCTAAATCTCTCGCACAATAATACACGGCCGTGTCA14300.10337471454482233No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGAGACCCCAGAGGGCCGCTGATTGACTTCA14210.1027241044532815No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT14070.10171204431088463No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13900.10048311413797416No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAATACCACAA13840.10004937407694693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATT507.625428E-491.65610570
TACGTAT702.6509848E-587.2915370
TAAATGT550.001113524883.3237270
AATGGTA3200.071.6063370
CTGGAGT19900.058.34056570
GCCCCTT800.004919660757.28506570
GGTAGAA29100.053.0198570
GAGCCAT17400.051.7979970
AGTAATA18250.044.36322470
GGTATCA9100.041.6603551
GCACCGT14450.040.17222670
GACAGGT3050.038.8431551
TATAAGA1350.037.5746882
GTATCAA23750.037.340831
TAAGTCA1700.002120966835.94356570
ATCCTAA3954.7366484E-934.80611470
ATGGCAA1800.002807313333.94670570
TTTAAGT2252.0434047E-433.94670570
AGTGGAA18000.033.94670570
TGACAGT37850.033.0946770
Produced by FastQC (version 0.11.5)