FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081321
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50930.4709979737746701No Hit
GAGTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGGAAGCCCCTAAGCTCCTGATCTATAAGGCG46840.4331738678893687No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT45860.42411087919313506No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40090.3707502212571475No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33660.3112859178726761No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC31140.2879810897966469No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30600.2829871980660692No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT30510.28215488277763956No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29480.2726294966989451No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28240.2611620416139148No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT27680.25598319093035277No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26430.24442325636883036No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA26230.24257366683898676No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24920.23045885541851124No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC24360.22528000473494922No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAACTGG23790.22000867457489495No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA23650.21871396190400444No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23350.21593957760923907No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23120.21381254964991891No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAACTGGTTGGCCTGGTA22920.21196296012007537No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22760.2104832884962005No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAA22260.20585931467159152No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21800.20160525875295124No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACGTTGATGGGACCCCACTTTCTAAATTAGACGCCTTATAGA21530.1991083128876624No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACA21070.19485425696902217No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20880.19309714691567076No Hit
CCTTATAGATCAGGAGCTTAGGGGCTTCCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTACTAATACTCTGA19570.18098233549519524No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19380.17922522544184383No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATG19070.17635836167058627No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC18840.17423133371126612No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA18650.1724742236579147No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18470.17080959308105548No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG18350.16969983936314934No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17830.164890906585556No Hit
GCTTAGGGGCTTCCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTACTAATACTCTGACTGGCCCGGCAAGTGA16900.1562903152717833No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG16470.15231369778261958No Hit
GTATAAAAATTATAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCT16230.1500941903468073No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16040.14833708029345588No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15620.14445294228078434No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15510.14343566803937036No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC15440.1427883117039251No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15400.1424183937979564No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15120.13982896845617537No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15030.13899665316774576No Hit
GTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGGAAGCCCCT14960.1383492968323005No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14850.1373320225908865No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14480.13391028196067586No Hit
TTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC14430.133447884578215No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14390.13307796667224625No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAAGTAGG14280.1320606924308323No Hit
CCCCTAAGCTCCTGATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCAACGTTCAGCGGCAGTGGATC14080.1302111029009887No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAA14000.12947126708905127No Hit
TCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCAACGTTCAGCGGCAGTGGATCTGGGACAGAATTCAC13970.1291938286595747No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13960.12910134918308253No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13910.12863895180062165No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT13740.12706680070025458No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13620.12595704698234844No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG13360.12355258059355177No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13210.12216538844616907No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13090.12105563472826293No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13040.12059323734580205No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG12990.12013083996334113No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA12950.1197609220573724No Hit
GATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCAACGTTCAGCGGCAGTGGATCTGGGACAGAATTCA12910.1193910041514037No Hit
ACTTATTACTGCCAACAGTATAAAAATTATAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTG12720.11763389409805229No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT12490.11550686613873216No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12250.11328735870291987No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG12240.11319487922642768No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG12070.11162272812606062No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11710.10829346697234217No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11710.10829346697234217No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG11620.10746115168391254No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11480.10616643901302204No Hit
GGTATCAGCAGAAACCAGGGGAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCA11470.10607395953652984No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11420.10561156215406896No Hit
GTATTACTGTGCGAGACGCCCAGACTACGGTGACTACGGCTACTGGTACTTCGATCTCTGGGGCCGTGGCACC11280.10431684948317844No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGA11250.1040394110537019No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11230.10385445210071756No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAAAAATTATAACACTTT11230.10385445210071756No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTG11210.10366949314773317No Hit
GATCAGGAGCTTAGGGGCTTCCCCTGGTTTCTGCTGATACCAGGCCAACC11000.10172742414139742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1750.052.8881640
TGTTAGA14550.051.8569670
CGTATGC1750.050.97221441
TATGGCG352.3250046E-549.09669543
CGCGTAT352.369799E-548.9385139
ACTACGC3200.048.85655227
CTACGCT3200.047.8143928
ACGAGAC3400.045.9294422
ATCTCGT1900.044.98671337
GAGACTA3550.044.95752324
CACGAGA3400.044.928921
TACGCTG3550.044.08055529
CGAGACT3550.043.99894323
TATACAC3850.043.180383
CCCACGA3700.040.3610819
TTATACA4050.040.208442
CTTATAC4150.040.0644651
CGTATGG603.0404226E-740.02625741
ATGCCGT2250.039.74728444
TTAACGG702.4096153E-839.00147635
Produced by FastQC (version 0.11.5)