FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596271
Sequences flagged as poor quality0
Sequence length19-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATGTAGACTCTGTGAAGGGCCGATTCATCATCTCCAGAGACAACGCCAAGAAGTCCCTGTATCTGCAAATGAA56550.35426315456460716No Hit
CAGTAATACAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGGGACTTCTTGGCGTTGTCTC45320.2839116916864367No Hit
GTCTACATAGCGTGCACTACCACCATTCCAATTAATACCAGAGACCCACT42720.2676237305570295No Hit
ATGTAGACTCTGTGAAGGGCCGATTCATCATCTCCAGAGACAACGCCAAG35190.22045128928609242No Hit
GTATTACTGTGGGGGGGGCAGCAGTGGCTGGTTCCACTACTTTGACTACTGGGGCCAGGGAACCCCGGTCACCG33680.21099174263016743No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG32750.20516566422618715No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA30830.19313763139216336No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT29980.18781272102293406No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28270.17710025428013162No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCC28260.17703760827578774No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCTCGAGAGTGGTACTATTTGTATA27570.17271503397606047No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC27440.1719006359195901No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG27410.17171269790655846No Hit
GATTATTACTGCAGCTCATATACAAATAGTACCACTCTCGAGGTGGTATTCGGCGGAGGGACCAAGCTGACCGTC26870.16832981367198926No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT26440.1656360354852027No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG25520.1598726030855663No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24220.15172862252086267No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23930.14991188839489034No Hit
GCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTC23810.14916013634276387No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGATGATTACGGCATGGGCTGGGTCCGCCAAGCTCCAGGGAAGGGG23680.1483457382862935No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA23590.14778192424719863No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC23560.147593986234167No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT23550.14753134022982314No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT23410.1466542961690089No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22390.14026440372593374No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA21660.13569124540883096No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT21470.13450097132629735No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC21290.13337334324810762No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG21060.13193248514819853No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAATAGTACCACTCTCGAGGTGG21020.13168190113082304No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC21010.13161925512647915No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG20850.13061691905697717No Hit
ATACAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGGGACTTCTTGGCGTTGTCTCTGGAGA20770.1301157510222262No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG20600.1290507689483803No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19860.12441496462693363No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG19390.12147060242277156No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAG19330.12109472639670832No Hit
GAGTCTACATAGCGTGCACTACCACCATTCCAATTAATACCAGAGACCCACTCCAGCCCCTTCCCTGGAGCT19290.12084414237933283No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19150.1199670983185186No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18560.11627098406223003No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGCTTCTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC18360.11501806397535255No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18230.11420366591888219No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCTCGAGAGTG18130.11357720587544347No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18080.11326397585372408No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18060.11313868384503635No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACAT18010.11282545382331696No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC17910.11219899377987823No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT17890.11207370177119047No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17870.11194840976250274No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGCTTCTTATAACTATGTCTCCTGGTACCAACAACA17840.11176047174947111No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC17840.11176047174947111No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG17570.11006902963218651No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17390.10894140155399679No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC17160.10750054345408767No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC16980.10637291537589795No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16900.10587174734114696No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16850.10555851731942759No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16420.102864739132641No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16250.10179975705879515No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG16100.10086006699363703No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG16100.10086006699363703No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAG16020.10035889895888607No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA500.00336539163.05558470
AGGGGGG2850.058.9993770
GTATAAA306.936019E-445.1284681
TTGGCGG27400.038.7385370
GTATAAC350.001480849738.681541
CTAATAT350.001483588538.6670043
GGTATCA6950.038.4728281
GTATCAA17050.037.9158481
GACAGGT3300.036.9232941
ATCAACG19700.032.459673
TGGTATC2100.032.2214932
TCAACGC19900.032.135464
TATCAAC19900.031.9624232
CAACGCA20300.031.5022455
AAATGAA23500.031.23787969
AACGCAG20550.031.1190096
CTTATAC2000.030.4617161
ACGCAGA21200.030.1629967
TCTCGCG18500.030.10761870
TCGCATT450.005085937730.07433518
Produced by FastQC (version 0.11.5)