FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546025
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT66150.42787147685192667No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62910.40691450655713846No Hit
GTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC56500.36545334001714075No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49990.3233453534063162No Hit
GGCTAACACTTTCCCTCCAAAAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCA48970.31674778868388287No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT42740.27645089827137337No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41890.2709529276693456No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA37950.24546821687876974No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37650.24352775666628937No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37010.23938810821299786No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGCTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAA36990.23925874419883247No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35980.23272586148348184No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35350.228650895037273No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33510.2167494057340599No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC33350.21571449362073705No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC32960.21319189534451255No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31950.20665901262916186No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG31180.20167849808379554No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30050.19436943128345274No Hit
GGCTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATC29990.19398133924095665No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTTTTTGGAGGGAAAGTGTTAGCCTGT29860.1931404731488818No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT29260.189259552723921No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29100.18822464061059815No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACA28710.18570204233437365No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC28120.18188580391649553No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC27650.17884574958360958No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27620.17865170356236154No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC26800.1733477789815818No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACACTTTC25780.16675021425914846No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24460.15821218932423475No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22500.1455345159360295No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22450.1452111059006161No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21490.13900163322067882No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGCATAGA21320.1379020391002733No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCCAGATGACCCAGTCTCCATCTTCCG21290.13770799307902523No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20770.13434452871072589No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACC20450.13227470448408013No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20000.12936401416535956No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAT19870.12852314807328472No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19860.12845846606620204No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGG19760.12781164599537523No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGCTATTAGCAG19730.1276175999741272No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19720.12755291796704452No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19560.12651800585372164No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18680.12082598923044581No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA18290.11830339095422131No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17940.11603952070632752No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT17660.11422842450801247No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG17550.11351692243010301No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17490.11312883038760693No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAAC17410.1126113743309455No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT17290.11183519024595334No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17250.11157646221762262No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17110.1106709141184651No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA17050.11028282207596901No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16870.10911854594848078No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTT16850.10898918193431542No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT16740.10827767985640595No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTC16610.1074368137643311No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16610.1074368137643311No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGC16480.10659594767225627No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16400.10607849161559484No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16150.10446144143852784No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC16140.10439675943144516No Hit
GGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAAAG16130.10433207742436248No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15990.10342652932520495No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15930.10303843728270887No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15900.10284439126146085No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15870.10265034524021281No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC15730.10174479714105528No Hit
ATTGTCAACAGGCTAACACTTTCCCTCCAAAAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTG15670.1013567050985592No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGAG803.9909352E-450.4755270
TGTTAGA18200.042.15538470
AATGGTA5250.040.38041770
CGTATGC1700.038.3167241
TAGTACG451.1458667E-437.467894
TAAGGCG2350.037.4441229
GACTAAG2300.036.71037726
TCGTATG1800.036.1475940
GCGTAGA37600.034.9032970
GGCGAAT2350.034.6335232
GTATCAA36550.034.415281
CGAATCT2000.033.96391734
CAGTTCG28000.033.53016770
GCGAATC2050.033.1085633
CACGAGA2800.031.32157321
CGTCGGA2601.7800994E-731.06186170
GTGGTGA7500.030.95831970
AGACTAA2750.030.68930225
ACGAGAC2800.030.12176922
ACTAAGG2950.029.78388427
Produced by FastQC (version 0.11.5)