FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963269
Sequences flagged as poor quality0
Sequence length19-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35540.36895197499348575No Hit
GATTATTACTGTGCTGCATGGGATGACAGCCTGAATGGTCCGATATTCGGCGGAGGGACCAAGCTGACCGTCCTA26550.2756239430522523No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT24430.25361555287256204No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCC24100.25018971855213856No Hit
GAAATATACGGCCGTGTCATCGGCTCTCAGTCTGTTCATTTGCAAATACAGCATGTTCTTGGAATTGTCTCTGGA22570.2343063048847207No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21420.222367791343851No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGTCAGAGGGTCAC20710.21499705689687929No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGTCAGAGGGTCACCATCTCTTGTTCTGGAAGCA20630.21416655160707965No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT19340.2007746538090606No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGACGACTATGCCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGG19070.19797169845598683No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18300.1899780850416654No Hit
CTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACATGCTGTATTTGCAAATGAA18040.1872789428498166No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG17420.18084252685386948No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG17200.17855863730692048No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17180.1783510109844706No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16590.1722260344721983No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16360.16983833176402438No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC16310.16931926595789962No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16100.16713918957217558No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGTCA15950.1655819921538013No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15940.16547817899257633No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC15410.15997608144765377No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGA15290.15873032351295432No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14970.1554083023537558No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCGGACCATTCAGGCTGTCATCCCATGCA14870.15437017074150627No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT14790.15353966545170664No Hit
GTATATTTCTGTGCGAAAGGGTCTTACGATTTTTGGAGTGGTTATCCCTTTGACTACTGGGGCCAGGGAACCCGG14770.15333203912925672No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG13930.1446117335863606No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT13580.14097827294348725No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13500.14014776765368758No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13400.13910963604143808No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAT13390.1390058228802131No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA13290.13796769126796357No Hit
GTCTGCGTAGTGTGTGCTTCCACCACTACCACTAATTCCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCG12950.13443804378631513No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC12900.13391897798019037No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA12830.1331922858516157No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12810.1329846595291658No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12690.13173890159446636No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT12620.13101220946589168No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCGGACCATTCAGG12540.13018170417609204No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGT12470.12945501204751736No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCA12210.12675586985566856No Hit
GGGATAACCACTCCAAAAATCGTAAGACCCTTTCGCACAGAAATATACGGCCGTGTCATCGGCTCTCAGTCTGTT12090.12551011192096911No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGG12070.12530248559851923No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11930.12384910134136985No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCCGGGTTCCCTGGCCCCAGTAGTCAAAGGGATAACCA11720.12166902495564583No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC11710.12156521179442087No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG11680.12125377231074601No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG11450.11886606960257208No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT11430.11865844328012216No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG11420.11855463011889722No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGT11410.11845081695767226No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACA11350.11782793799032254No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11330.11762031166787264No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCTGCATGGGATGACAGCCTGAATGGTCCGATATTCGGCGGAGGGAC11270.11699743270052292No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGGTGGTGGAGT11190.11616692741072328No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATGGCATAGTCGTCAAAGGTGAATCCAGAGGCTGCACA11010.11429829050867411No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG10990.11409066418622421No Hit
GGGCTGGAGTGGGTCTCAGGAATTAGTGGTAGTGGTGGAAGCACACACTA10880.1129487194127497No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGG10730.1113915219943754No Hit
GTCTGGGGGAGGCTTGCTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTGAC10650.11056101670457578No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT10620.11024957722090092No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT10560.1096266982535512No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTGACGACTATGCCATGACCTGGGTCCGCCAGGCTCCAG10530.10931525876987634No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC10420.10817331399640183No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCCGGGTTCCCTG10400.10796568767395193No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT10380.10775806135150201No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA10340.1073428087066022No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG10330.10723899554537725No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT10310.10703136922292734No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA10150.10537035864332808No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGC10140.10526654548210312No Hit
CATCTATACTAATAATCACCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC9980.10360553490250386No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGA9940.10319028225760406No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9910.1028788427739292No Hit
GTGTGTGCTTCCACCACTACCACTAATTCCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGT9910.1028788427739292No Hit
GTCCGATATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC9780.1015292716780048No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAG9650.1001797005820804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA2000.0107.85895570
GTAGTTT651.2156934E-5102.11570
CAAGCAT750.002743707766.3747470
ATTTAAT850.004504848358.56595270
GGTAGAA17600.049.96962470
AGCTCCA17300.049.8769770
TAGGCAA2355.757829E-849.42799870
AAAGGTT1354.5362845E-449.1664870
GTGGTAT1500.047.0296671
GGTATCA6750.045.287831
GAGTAAT307.1965106E-444.790161
GAGCCAT11950.044.4349870
CTCTTGT21350.039.63831370
TATACTG350.001543398438.3556945
TGGTATC1850.038.0965352
GTATCAA16950.037.853631
AAGCGGT7900.037.808470
ACTCAGT12550.034.37735770
TATCAAC18550.034.013542
GTGTATA400.002958501733.592621
Produced by FastQC (version 0.11.5)