FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431951
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGACGGATCAACCCTAACAGTCGTCG54580.38115829382429983No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT36270.2532907899781487No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33860.23646060514640513No Hit
GTGTATATAGTAATCGGTGAAGGTGTATCCAGAAGCCTTGCAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCAC33730.23555275285257665No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25250.1763328493782259No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGA24560.17151424874175164No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT24180.16886052665209914No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24040.16788283956643768No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG23630.1650196131012863No Hit
GTGATGGAAACACCTACTTAAATTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATTCGGTT23470.1639022564319589No Hit
GTAATAAACTCCGACATCCTCAGCTTCCACCCTGCTGATTTTCAGTGTGAAATCTGTCCCTGCCCCACTGCCA23460.16383242164012596No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT23350.16306423892996338No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT23100.16131836913413936No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC22820.15936299496281645No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCA21660.15126215911019303No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA21060.14707207160021538No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21000.1466530628492176No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGG20770.14504686263705951No Hit
GCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGGCTCGTA20530.14337082763306847No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20170.14085677512708186No Hit
GTTTATTACTGCATGCAAGCTACACAGGAACCGAACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAA20010.13973941845775448No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC20010.13973941845775448No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19740.13785387907826455No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19630.137085696368102No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19120.133524121984621No Hit
GAGTTACAGCGAATCCTCTGGCACAGAAATACACGGCCGTGTCGTCAGATCTCAGTCTGGTTAGCTCCATGTAGG19080.13324478281728913No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18970.13247660010712656No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGCGACAGTCGGGGG18970.13247660010712656No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC18530.1294038692664763No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGG18480.12905469530731148No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG18190.12702948634415562No Hit
GAAATACACGGCCGTGTCGTCAGATCTCAGTCTGGTTAGCTCCATGTAGGCTGTGCTGATGGACGTGTCCCTGG18190.12702948634415562No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17700.12360758154434057No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC17540.12249022487501318No Hit
GGCCAGGCCAGCCTCCAAGACTCCTAATTTATTCGGTTTCTAACCGGTTCTCTGGGGTCCCAGACAGATTCAG17170.11990633757719363No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17150.11976666799352771No Hit
GTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAG17140.11969683320169475No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17080.11927782445069698No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16810.11739228507120704No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16660.11634476319371263No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA16390.11445922381422269No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG16390.11445922381422269No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGACGGATCAACCCTAACAGTCGT16370.11431955423055677No Hit
GTCTGGGACCCCAGAGAACCGGTTAGAAACCGAATAAATTAGGAGTCTTGGAGGCTGGCCTGGCCTCTGCTGAAG16180.11299269318573052No Hit
GGCCAAAAGTGTTCGGTTCCTGTGTAGCTTGCATGCAGTAATAAACTCCGACATCCTCAGCTTCCACCCTGCTGA16020.11187533651640315No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT15720.10978029276141432No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC15530.10845343171658807No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGT15480.10810425775742327No Hit
CCCTAACAGTCGTCGCACAATTTATGCACAGAAGTTTCAGGGTAGGGTCACCGTGACCAGGGACACGTCCATCAG15430.10775508379825846No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15080.10531086608410484No Hit
GGGTTCCCTGGCCCCAGGCGTCAAAGTAGGGCCGACGGGCCTGAGTTACAGCGAATCCTCTGGCACAGAAATACA14990.1046823529576082No Hit
GTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATTTCACACTGAAAATCAGCAGGGTGGAAGCTGAGGA14910.1041236746229445No Hit
CCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTG14410.10063193503129647No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14340.10014309148846574No Hit
CTTCTCACCTGTCAGCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGGCTCGTACACAGTGA14330.10007325669663276No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)