FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431951
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGACGGATCAACCCTAACAGTCGTC52330.3654454656618837No Hit
GTGTATATAGTAATCGGTGAAGGTGTATCCAGAAGCCTTGCAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCAC42670.2979850567512436No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA33800.2360415963954074No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33350.23289903076292415No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28800.201124200478927No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACC25150.17563450145989631No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23180.16187704746880305No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC22850.15957249933831535No Hit
GTGATGGAAACACCTACTTAAATTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATTCGGTTT22600.15782662954249133No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT22320.15587125537116842No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21940.1532175332815159No Hit
GTAATAAACTCCGACATCCTCAGCTTCCACCCTGCTGATTTTCAGTGTGAAATCTGTCCCTGCCCCACTGCCA21380.14930678493887012No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC20600.14385967117589918No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG20560.14358033200856732No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC20540.14344066242490142No Hit
GAGTTACAGCGAATCCTCTGGCACAGAAATACACGGCCGTGTCGTCAGATCTCAGTCTGGTTAGCTCCATGTAG19990.13959974887408855No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT19420.1356191657396098No Hit
GTTTATTACTGCATGCAAGCTACACAGGAACCGAACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAA19250.13443197427844947No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT19220.1342224699029506No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18780.13114973906230032No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAG18550.12954353885014222No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGG18460.12891502572364558No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18140.12668031238499083No Hit
GAAATACACGGCCGTGTCGTCAGATCTCAGTCTGGTTAGCTCCATGTAGGCTGTGCTGATGGACGTGTCCCTGGT18010.12577246009116233No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG17830.12451543383816903No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA17440.12179187695668357No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC17380.12137286820568581No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA16990.11864931132420035No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16840.11760178944670593No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGACGGATCAACCCTAACAGTCGTCGCACAATTTAT16760.11704311111204223No Hit
GGGTTCCCTGGCCCCAGGCGTCAAAGTAGGGCCGACGGGCCTGAGTTACAGCGAATCCTCTGGCACAGAAATACA16710.11669393715287744No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC16520.11536707610805119No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA16500.11522740652438526No Hit
GCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGGCTCGTA16490.1151575717325523No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16480.11508773694071935No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTTCAGGGCCAGCTCCTCCGACGGCGGC16470.11501790214888637No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGACGGATCAACCCTAACAGTCGT16110.11250384964289978No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC15790.11026913630424505No Hit
GTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCA15730.10985012755324729No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGCGTCAAAGTAGGGCCGAC15720.10978029276141432No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAACGTGGTCGTCGCATGCCTGGTCCAGG15710.10971045796958137No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15660.10936128401041656No Hit
GGCCAGGCCAGCCTCCAAGACTCCTAATTTATTCGGTTTCTAACCGGTTCTCTGGGGTCCCAGACAGATTCAGTG15440.10782491859009141No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15290.10677739671259702No Hit
GTCTGGGACCCCAGAGAACCGGTTAGAAACCGAATAAATTAGGAGTCTTGGAGGCTGGCCTGGCCTCTGCTGAA15290.10677739671259702No Hit
GGCCAAAAGTGTTCGGTTCCTGTGTAGCTTGCATGCAGTAATAAACTCCGACATCCTCAGCTTCCACCCTGCT15230.10635838796159923No Hit
CCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCA15130.10566004004326963No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC15100.10545053566777077No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14990.1046823529576082No Hit
GTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGG14900.10405383983111154No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14790.10328565712094896No Hit
GTGCTGATGGACGTGTCCCTGGTCACGGTGACCCTACCCTGAAACTTCTGTGCATAAATTGTGCGACGACTGTTA14780.10321582232911601No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA14710.10272697878628528No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGCGACAGTCGGGGGT14560.10167945690879086No Hit
CCATGTAGGCTGTGCTGATGGACGTGTCCCTGGTCACGGTGACCCTACCCTGAAACTTCTGTGCATAAATTGTGC14520.10140011774145902No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT14500.1012604481577931No Hit
CTTCTCACCTGTCAGCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGGCTCGTACACAGTGATG14470.10105094378229423No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT601.4851827E-598.1007770
AATGGTA8300.067.3704170
AGTAACA1806.850288E-540.87532470
GGTATCA10600.040.346311
CCGGGTT1858.050879E-539.7705870
AGTTTCA18100.038.2105270
GTGGTAT3300.038.056331
AACTGTA2459.937887E-636.03701870
GAGGGTT2459.937887E-636.03701870
GTATCAA26200.035.7558361
CAGCCAT19100.034.66912570
AAGGACA18850.033.5676470
AAGCGGT13850.032.9363670
CGTCACA8950.032.88294270
TCTTCAC19350.032.54808469
TGGTATC3800.032.1309972
TATCAAC30000.031.2027662
TAAGTCA2852.7973878E-530.9791970
ACGGATA450.00451683730.81269552
GAGCAAA18750.030.6074470
Produced by FastQC (version 0.11.5)