FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences967338
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGTACTATGGTACCTCAGGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTG27900.2884203866693958No Hit
CTATTAGGGTTGATCCGTCCCATGTACTCAAGCCCTTGTCCAGGGGCCTG24750.2558567946260769No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22780.23549162753866798No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22350.23104643878354825No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20870.21574671934732223No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT19560.20220440011660865No Hit
CCATAAAGGCTGTGCTGATGGACGTGTCGCTGGTCAGGGTGACCCTGTCCTGAAACTCCTGTGCAAAATTTG18910.1954849287425905No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18100.18711143364573704No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17310.17894469151423803No Hit
GTACTATGGTACCTCAGGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCA17180.17760079723943442No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14840.15341070029296897No Hit
CCATAGATGAGGAGCCTGGGAGGCTGGCCAGGTCTCTGCTGGTACCAGGCTAAGTAGTTGCTGCCAAGCGTCTGA14810.15310057084493733No Hit
CCCTAATAGTGGTGCCACAAATTTTGCACAGGAGTTTCAGGACAGGGTCACCCTGACCAGCGACACGTCCATCAG14370.1485520056071404No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT14330.1481384996764316No Hit
ATAATATACGGCCGTGTCGTTAGATGTCAGCCCAATAATCTCCATAAAGGCTGTGCTGATGGACGTGTCGCTGG14020.14493382871343832No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13770.14234941664650827No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13610.14069539292367303No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13470.13924812216619217No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13230.1367670865819393No Hit
CCCTAAAGAGACGATGACCAGAGTCCCTTGGCCCCAGATATCAAAAGCATCACCCCTTTGTCCCCATTCGAGAAA12940.1337691685843004No Hit
GGGTGACCCTGTCCTGAAACTCCTGTGCAAAATTTGTGGCACCACTATTA12680.13108138003469313No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACAA12430.12849696796776308No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12400.12818683851973145No Hit
CCATAGTACTGACAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCT12390.12808346203705426No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12330.12746320314099105No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12050.12456866162602936No Hit
CATCTATGGTGCGTCCAGCAGGGCCACTCGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA12030.12436190866067497No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12010.12415515569532055No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCGAGTGGCCCTGCTGGACGCACCATAGATGAGGAGCC12000.12405177921264335No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11950.12353489679925735No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA11710.12105386121500447No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11650.12043360231894125No Hit
CTCCTGCAGGGCCAGTCAGACGCTTGGCAGCAACTACTTAGCCTGGTACC11010.11381750742760029No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACGCTTGGCAG10890.11257698963547384No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC10880.11247361315279664No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT10650.11009595405122097No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10280.10627102419216447No Hit
GGGTCACCCTGACCAGCGACACGTCCATCAGCACAGCCTTTATGGAGATTATTGGGCTGACATCTAACGACACGG9990.10327310619452561No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCGAGTGGCCCTGCTGGACGCACCATAGA9780.10110220005830432No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC9780.10110220005830432No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC9720.10048194116224111No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9680.10006843523153232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACG5650.049.9096270
GTGGTAT1150.048.997521
TGTTAGA10250.044.44109370
TAGGCGG14750.043.38297370
TGGTATC1400.040.2209132
GTATCAA12350.037.8420941
TATTCTA1150.0016726437.72432370
AGTAAGA1150.0016726437.72432370
GGTATCA5300.036.2723051
GCGTAGA14500.034.40718570
TCCGAGA8250.034.18052370
CAGTTCG10000.033.62180370
GCTCGGG3953.6379788E-1232.94909370
AATGGTA2351.6213507E-632.3064770
CTTATAC1157.4578566E-1131.7042771
ATCAACG14850.031.2271733
CAACGCA15000.030.91495
TCAACGC15000.030.91494
TATCAAC15350.030.4257852
AACGCAG15250.030.4080986
Produced by FastQC (version 0.11.5)