FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences967338
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGTACTATGGTACCTCAGGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTG26320.27208690240639777No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA22110.22856540319929536No Hit
CTATTAGGGTTGATCCGTCCCATGTACTCAAGCCCTTGTCCAGGGGCCTGTCGCACCCAGTGGATATAGTAGGCG21920.22660125002842854No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAGGAAGATGCTGGCAAAGGATGG20490.21181841300558854No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19380.200343623428419No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19280.19930985860164696No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17110.176877161860694No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA16920.17491300868982712No Hit
GGGTGACCCTGTCCTGAAACTCCTGTGCAAAATTTGTGGCACCACTATTAGGGTTGATCCGTCCCATGTACTCAA16740.1730522320016375No Hit
CCATAAAGGCTGTGCTGATGGACGTGTCGCTGGTCAGGGTGACCCTGTCCTGAAACTCCTGTGCAAAATTTGTG16180.16726314897171413No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15880.16416185449139803No Hit
GTACTATGGTACCTCAGGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT15620.16147406594179078No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT14290.14772499374572282No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC14130.14607097002288755No Hit
CCCTAATAGTGGTGCCACAAATTTTGCACAGGAGTTTCAGGACAGGGTCACCCTGACCAGCGACACGTCCATCA13960.14431356981737511No Hit
ATAATATACGGCCGTGTCGTTAGATGTCAGCCCAATAATCTCCATAAAGGCTGTGCTGATGGACGTGTCGCTGGT13660.14121227533705902No Hit
CCATAGATGAGGAGCCTGGGAGGCTGGCCAGGTCTCTGCTGGTACCAGGCTAAGTAGTTGCTGCCAAGCGTCT13650.14110889885438183No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTC12750.13180501541343356No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC12740.13170163893075637No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12530.12953073279453511No Hit
CCATAGTACTGACAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCA12510.1293239798291807No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGA12220.12632606183154182No Hit
CCCTAAAGAGACGATGACCAGAGTCCCTTGGCCCCAGATATCAAAAGCATCACCCCTTTGTCCCCATTCGAGAAA12010.12415515569532055No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11990.12394840272996616No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11930.12332814383390292No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11840.12239775548980811No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA11690.12084710824965007No Hit
CATCTATGGTGCGTCCAGCAGGGCCACTCGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA11220.11598841356382154No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACGCTTGGCA11150.11526477818508113No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC11070.1144377663236635No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11020.11392088391027748No Hit
CTCCTGCAGGGCCAGTCAGACGCTTGGCAGCAACTACTTAGCCTGGTACCAGCAGAGACCTGGCCAGCCTCCCAG10860.11226686018744224No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCGAGTGGCCCTGCTGGACGCACCATAGATGAGGAGCCTG10840.11206010722208783No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC10790.11154322480870181No Hit
GGGTCACCCTGACCAGCGACACGTCCATCAGCACAGCCTTTATGGAGATTATTGGGCTGACATCTAACGACACG10640.10999257756854379No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA10310.10658115364019609No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA10300.10647777715751888No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10050.10389336509058882No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10000.1033764826772028No Hit
GTCCAGGGGCCTGTCGCACCCAGTGGATATAGTAGGCGGTGAAGGTGTATCCAGAAGCCTTGCAGGAGACCTTCA9840.10172245895436756No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCGAGTGGCCCTGCTGGACGCACCATA9800.10130895302365875No Hit
GTGGATATAGTAGGCGGTGAAGGTGTATCCAGAAGCCTTGCAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCAC9700.10027518819688672No Hit
GGTGTATCCAGAAGCCTTGCAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCACCTCAGCCCCAGATTGCAGCA9690.1001718117142095No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)