FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925202
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAGACCTGGCCGTACCATTTTGGCCAGGGGACCAAGCTCGACATCAAACGAACTGTGGCTGCACCA40340.4360128923197312No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34220.36986517538872593No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT31540.3408985281052138No Hit
GTCTTATATTGCTGACATGAATAGAGGCCAACATCCTCAGACTGCAGGCCACTGATGGTGAGAGTGAAGTCTGTC31210.3373317394471694No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA22800.24643267091943163No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT21200.22913915015315575No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21100.22805830510526348No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21080.22784213609568504No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19810.21411540398745357No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19750.2134668969587182No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18570.20071292539358973No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18340.1982269817834376No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17230.18622960175183365No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC16600.1794202779501125No Hit
CTCCTGCACGGCCAGTCAGAGTGTCAGTGGTAATGTGGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGC16580.17920410894053407No Hit
CTATTCATGTCAGCAATATAAGACCTGGCCGTACCATTTTGGCCAGGGGACCAAGCTCGACATCAAACGAACTG15880.17163819360528834No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15750.17023309504302844No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15740.17012501053823922No Hit
GGGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14460.15629019392521848No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT14060.1519668137336495No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC14000.15131830670491417No Hit
GAGTAGGAGTACTGGCCACTCCAAAACTCCTTCTTCCCCTCCGCATCTTTCGCGCAGAAATAAATGGCCGTGTC13950.15077788418096805No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13100.14159070127388398No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCACGGGAGAAATATTGA12970.14018560271162406No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG12820.1385643351397857No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12580.1359703070248443No Hit
GTCTCCAGCCACTCTGTCTGTGTCTCCAGGGGAGAGTGCCACTCTCTCCTGCACGGCCAGTCAGAGTGTCAGTGG12470.13478137747216284No Hit
GTCTGTGTCTCCAGGGGAGAGTGCCACTCTCTCCTGCACGGCCAGTCAGAGTGTCAGTGGTAATGTGGCCTGGTA12460.13467329296737363No Hit
GACTTCACTCTCACCATCAGTGGCCTGCAGTCTGAGGATGTTGGCCTCTATTCATGTCAGCAATATAAGACC12370.13370053242427057No Hit
TCCTCAGACTGCAGGCCACTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCACCGAATCTGCCTGAGACA12270.13261968737637836No Hit
GTGGTAATGTGGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGATTCTTCCACGAGGG12150.13132267331890765No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12030.13002565926143694No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12000.1297014057470693No Hit
GAGTGAAGTCTGTCCCAGATCCACTGCCACCGAATCTGCCTGAGACATTAGAGGCCCTCGTGGAAGAATCATAAA11890.12851247619438783No Hit
GGAGAAATATTGATGACGCAGTCTCCAGCCACTCTGTCTGTGTCTCCAGG11630.125702279069868No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11580.12516185654592185No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCGAGCTTGGTCCCCTGGCCAAAATGGTACGGCCAGG11360.12278399744055893No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11290.12202740590703436No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA11100.11997380031603909No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11050.11943337779209295No Hit
CTCCAGGGGAGAGTGCCACTCTCTCCTGCACGGCCAGTCAGAGTGTCAGT11010.11900103977293608No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10960.11846061724898994No Hit
GTGCAGCCACAGTTCGTTTGATGTCGAGCTTGGTCCCCTGGCCAAAATGG10590.11446149057178864No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10560.11413723705742096No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTCGGGCTGACCTGGCTTTTT10470.11316447651431795No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10250.11078661740895501No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10190.11013811038021967No Hit
GTCTGAGGATGTTGGCCTCTATTCATGTCAGCAATATAAGACCTGGCCGTACCATTTTGGCCAGGGGACCAAGC10180.11003002587543044No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT9930.10732791325569982No Hit
GTCAGAGTGTCAGTGGTAATGTGGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGAT9890.10689557523654294No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9840.10635515271259681No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA9830.10624706820780759No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA9720.10505813865512612No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9720.10505813865512612No Hit
ATTTTGGCCAGGGGACCAAGCTCGACATCAAACGAACTGTGGCTGCACCA9670.10451771613118001No Hit
CAGGTATACCGGGTAGTGGTGACAGGACATATTATGCAGACTCCGTGAAG9420.10181560351144939No Hit
CATGAATAGAGGCCAACATCCTCAGACTGCAGGCCACTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCA9410.10170751900666017No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9360.10116709648271403No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT9320.10073475846355716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAGA504.003648E-580.3300270
CGTCGGA1202.0008883E-1066.9416870
GGTATCA8700.051.3159941
GCGTAGA19850.041.9860970
TGTTAGA10300.040.94491270
GTATCAA24400.040.0595551
GTGGTAT1550.037.0949971
CGTATGC2550.036.36987741
TGGTATC1600.035.9202422
GGAATAA2802.4920155E-1035.86161470
TCGTTAA1401.480116E-435.86161470
GTGGCCA10900.035.006270
TAACCGT1201.8189894E-1234.8740153
TATTCTA2051.0187123E-634.28720570
GTCTATA400.00286143133.8219071
TCGTATG2550.033.6388740
CAGTTCG12850.033.60107870
TATCAAC30300.032.3568082
GTACCCT1305.456968E-1231.9085647
CACGAGA3200.031.71832821
Produced by FastQC (version 0.11.5)