FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925202
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAGACCTGGCCGTACCATTTTGGCCAGGGGACCAAGCTCGACATCAAACGAACTGTGGCTGCACCAT36930.3991560761866057No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32940.3560303587757052No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31970.34554616181115044No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA31680.34241171117226293No Hit
GTCTTATATTGCTGACATGAATAGAGGCCAACATCCTCAGACTGCAGGCCACTGATGGTGAGAGTGAAGTCTGTC30270.32717179599698226No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22960.24816202299605927No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT21270.2298957416866803No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA21170.22881489663878807No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA20860.22546427699032212No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC18690.20200993945106044No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17590.19012064392424574No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA16110.1741241372154405No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15930.1721786161292345No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC15930.1721786161292345No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC15760.1703411795478177No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA15560.16817948945203318No Hit
CTCCTGCACGGCCAGTCAGAGTGTCAGTGGTAATGTGGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT15350.16590971485145947No Hit
CTATTCATGTCAGCAATATAAGACCTGGCCGTACCATTTTGGCCAGGGGACCAAGCTCGACATCAAACGAACTGT14310.15466892635338014No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13880.1500212926474435No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13750.14861619408518356No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT13600.1469949265133452No Hit
GTCTGTGTCTCCAGGGGAGAGTGCCACTCTCTCCTGCACGGCCAGTCAGAGTGTCAGTGGTAATGTGGCCTGGTA13250.14321196884572235No Hit
GAGTAGGAGTACTGGCCACTCCAAAACTCCTTCTTCCCCTCCGCATCTTTCGCGCAGAAATAAATGGCCGTGTC13140.14202303929304086No Hit
GAGTGAAGTCTGTCCCAGATCCACTGCCACCGAATCTGCCTGAGACATTA12540.1355379690056874No Hit
GGGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12520.13532179999610894No Hit
TCCTCAGACTGCAGGCCACTGATGGTGAGAGTGAAGTCTGTCCCAGATCC12470.13478137747216284No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCACGGGAGAAATATT12310.13305202539553523No Hit
GTGGTAATGTGGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATTTATGATTCTTCCACGAGG12230.13218734935722146No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12030.13002565926143694No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12010.1298094902518585No Hit
GACTTCACTCTCACCATCAGTGGCCTGCAGTCTGAGGATGTTGGCCTCTATTCATGTCAGCAATATAAGACCTG11610.12548611006028954No Hit
GTCTCCAGCCACTCTGTCTGTGTCTCCAGGGGAGAGTGCCACTCTCTCCT11600.12537802555550032No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA11510.12440526501239729No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11300.12213549041182357No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11190.1209465608591421No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA10780.11651509616278392No Hit
CTCCAGGGGAGAGTGCCACTCTCTCCTGCACGGCCAGTCAGAGTGTCAGT10750.11619084264841624No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10650.115109997600524No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10400.11240788498079338No Hit
GGAGAAATATTGATGACGCAGTCTCCAGCCACTCTGTCTGTGTCTCCAGGGGAGAGTGCCACTCTCTCCTGCACG10260.11089470191374423No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCGAGCTTGGTCCCCTGGCCAAAATGGTACGGCCAGGT10110.10927343434190588No Hit
GTGCAGCCACAGTTCGTTTGATGTCGAGCTTGGTCCCCTGGCCAAAATGG9980.10786833577964595No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT9940.10743599776048907No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9820.10613898370301837No Hit
GTCTGAGGATGTTGGCCTCTATTCATGTCAGCAATATAAGACCTGGCCGTACCATTTTGGCCAGGGGACCAAGCT9700.10484196964554768No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTCGGGCTGACCTGGCTTTTT9630.1040853781120231No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC9490.10257219504497396No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9350.10105901197792481No Hit
CAGGTATACCGGGTAGTGGTGACAGGACATATTATGCAGACTCCGTGAAG9300.1005185894539787No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTT550.00138289678.8909670
TTACCGT200.0079987672.3167270
CGGAGTA650.002682845366.7538970
ATGGCAA1955.838956E-1059.33679270
TAGGCAA5700.048.21114370
GTGGCCA8750.046.282770
GGTATCA10450.043.64111
TGGTATC3000.041.6255042
TAGAATT2103.877034E-641.32383770
GTGGTAT3050.040.9807741
TAGCATT1450.001269203439.89887270
GAGCAAA17650.039.33373670
GAGACAA8150.039.04215270
GTATCAA26900.038.9302941
AAAAGGT6350.038.72075770
ACTTCCA3053.0559022E-837.93663870
CGAAACC751.3402555E-436.47839769
ATACGTA656.467162E-736.35051711
CATAAAT10050.035.97846670
CAGCCAT9000.035.3548470
Produced by FastQC (version 0.11.5)