FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823117
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT41320.5019942486912553No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37110.45084720641172515No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27560.3348248183429573No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25940.31514353366532344No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25590.3108914042596618No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT25380.30834012661626475No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA23990.2914530984052085No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAC23380.2840422442981982No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCACCTACTTAGCCTGGTA22770.2766313901911879No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22270.2705569196116712No Hit
TTATTATTGTCAGCAGCGTGGCAGCTGGCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTG22220.26994947255371954No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTCTGCACTTTATTATTGTCAGCAGCG22160.26922053608417756No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC21930.26642627961759996No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21030.25549223257446996No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20540.24953925140654368No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20290.24650201611678535No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGC20220.245651590235653No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20180.2451656325892917No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19690.23921265142136539No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC19620.23836222554023306No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19360.23520350083888442No Hit
GTCAGAGTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGA19080.23180179731435505No Hit
GAGTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC18030.21904540909737014No Hit
GTCTCTGGTGGCTCCATCAATGGTTACTACTGGAACTGGATCCGGCAGACCGCCGGGAAGGGACCGGAGTACAT17740.21552221616125047No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT17660.21455030086852783No Hit
GCCTAGAGCCTGAAGATTCTGCACTTTATTATTGTCAGCAGCGTGGCAGCTGGCCTCGGACGTTCGGCCAAGGGA17330.21054115028604684No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTCTGCACTTTATTATTGTCAGCAGCGTGGCAGC17240.20944774558173382No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGCCAG16970.20616753146879482No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA16710.20300880676744618No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC16610.20179391265154287No Hit
CAATAATAAAGTGCAGAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCG16510.2005790185356395No Hit
GTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC16300.19802774089224254No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAGGAGC16060.19511199501407456No Hit
GTCCCAGACCCACTGCCGCTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGATGAGGAGCC14610.17749603033347627No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14280.1734868797509953No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14110.17142155975395965No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCAC14050.17069262328441764No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13940.169356239756924No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA13930.16923475034533367No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13840.16814134564102065No Hit
GTGTATTACTGTGCGAGAGATACCCCTAGCGATTCGGGGACTTATTATGTTGACTACTGGGGCCAGGGAACCC13460.16352474800058803No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13350.16218836447309434No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCGCTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGA13160.15988006565287802No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12630.15344112683859037No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12350.15003942331406106No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12310.14955346566769973No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACACAGTC11940.14505835743885742No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGT11800.14335750567659275No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11740.14262856920705078No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT11440.13898388685934077No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11400.13849792921297946No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCA11190.13594665156958244No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11110.13497473627685977No Hit
CAGTAATACACGGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGAGAACTGGTTCTTGGACGTGTCTA11080.13461026804208878No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11070.13448877863049846No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11060.13436728921890811No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG10960.1331523951030048No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10960.1331523951030048No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10830.13157303275233045No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCACCTACTTAGCCTGGTA10740.13047962804801747No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGGTGGTAACACTCTGAC10540.12804983981621082No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10230.12428366805691049No Hit
CCCAGATACCACCGGAGAAGTTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCAC10200.1239191998221395No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT10010.12161090100192316No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT9850.11966707041647785No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC9790.11893813394693586No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGCGGCAGTGGGTC9790.11893813394693586No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT9710.1179662186542132No Hit
GTATTACTGTGCGAGAGATACCCCTAGCGATTCGGGGACTTATTATGTTGACTACTGGGGCCAGGGAACCCTGG9700.11784472924262286No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT9590.1165083457151292No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9580.11638685630353887No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9580.11638685630353887No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGG9570.11626536689194854No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9520.11565791983399686No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACACAGTCTC9510.11553643042240654No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9390.11407855748332253No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9360.11371408924855154No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG9340.11347111042537088No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCT9330.11334962101378054No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG9310.11310664219059989No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9280.11274217395582888No Hit
CTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC9270.11262068454423854No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAATGGTTACTACTGGAACTGGA9170.1114057904283352No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9110.11067685395879322No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9100.1105553645472029No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT9080.11031238572402223No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGGTGGTA9070.1101908963124319No Hit
GGCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC9020.10958344925448021No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9020.10958344925448021No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACACAGTCTCCAGCCACCCTGTCT8960.10885451278493824No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8950.1087330233733479No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8950.1087330233733479No Hit
CACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCCTTCCCCTGGAGACAAA8940.10861153396175757No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8900.10812557631539622No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGT8890.10800408690380589No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCGCTGAACCTGGCTGGGATGCCAG8880.10788259749221557No Hit
GCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATC8670.10533131984881856No Hit
CCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACT8640.10496685161404758No Hit
CTGCAGGAGAGGGTGGCTCCTTCCCCTGGAGACAAAGACAGGGTGGCTGG8580.10423791514450559No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8560.10399493632132491No Hit
GGTTTCTGTTGGTACCAGGCTAAGTAGGTGGTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCCTTCCC8550.10387344690973457No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8540.10375195749814424No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC8510.10338748926337325No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG8480.10302302102860225No Hit
ACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCAC8350.10144365867792793No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG8340.1013221692663376No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8280.10059323279679559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)