FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537939
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT15730.2924123367147576No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14470.2689896066282608No Hit
CTATTACCTTACCTTCGGCCAAGGGACACAACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCT12890.23961824667852674No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12370.22995172315076617No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT11630.21619551659202993No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT11610.2158237272255776No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11590.2154519378591253No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11210.20838793989653104No Hit
GTCTGAGAGTCAGGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTT11100.20634309838104323No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10930.20318288876619842No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10400.19333047055521166No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9840.18292036829454641No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG9400.17474100223259514No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATG9330.173439739450012No Hit
GATCTATGCTGCATCCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCA9290.17269616071710733No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC8990.1671193202203224No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8950.1663757414874177No Hit
ACTACAACCCGTCCCTCCAGAGTCGAGTCACCATATCGCTAGACACGTCC8930.1660039521209654No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA8900.1654462680712869No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8730.1622860584564421No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGCCGGCGTAAT8690.16154247972353744No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTAGAAATGCTTTAGGCTGGTATCAGCAGAAA8640.1606130063074066No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8630.16042711162418044No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTAGAAA8560.15912584884159728No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8410.1563374285932048No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8410.1563374285932048No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGCCGGCGTAATTATTCCCCATACCTCTCGTACAGAAATAAACGGCCGTG8400.15615153390997863No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8220.15280542961190768No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8150.15150416682932452No Hit
GTCTCTGACTACTCCATCACCAGTGGTTACAACTGGGGTTGGATCCGGCAGCCCCCAGGAAAGGGGCTGGAG8040.1494593253138367No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8030.14927343063061055No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG7990.14852985189770587No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG7900.14685679974867039No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCG7870.1462991156989919No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCCTAAAGCATTTCTAATGTCCTGACT7850.14592732633253958No Hit
ACTTATTACTGTCTGCAAGATCACTATTACCTTACCTTCGGCCAAGGGACACAACTGGAGATTAAACGAACTG7820.14536964228286106No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGTCA7730.14369659013382557No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7720.1435106954505994No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTGCAAGATCACTATTAC7600.14127995925188544No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7590.14109406456865928No Hit
AGGTAATAGTGATCTTGCAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAA7580.14090816988543312No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7570.14072227520220695No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7490.13923511773639763No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC7340.13644669748800514No Hit
CAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCG7330.13626080280477898No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC7250.13477364533896966No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7240.1345877506557435No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT7220.13421596128929117No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7150.132914698506708No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTTGTGTCC7140.13272880382348184No Hit
AGTCAGGACATTAGAAATGCTTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTA7080.13161343572412484No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC6990.12994038357508936No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6980.12975448889186322No Hit
GTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTC6800.12640838459379225No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG6790.12622248991056606No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6590.12250459624604276No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG6580.1223187015628166No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT6560.12194691219636426No Hit
ACTCTGGAGGGACGGGTTGTAGTAGGTGCTCCCACCATGAGCGCTACTCC6540.12157512282991194No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG6520.1212033334634596No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG6510.12101743878023344No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC6450.11990207068087647No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCCTAAAG6440.11971617599765029No Hit
CAACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAA6420.11934438663119797No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA6340.11785722916538866No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGTCATCCAGATGACCCAGTCTCCATCCTCC6310.11729954511571015No Hit
GGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCGATGTCTCTGACTACTCCATCACCAGTGGTTACAA6290.11692775574925782No Hit
GTCAGGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGAT6180.11488291423377No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC6080.11302396740150834No Hit
GTACGGGGCTGTCTGAGAGTCAGGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGG6050.11246628335182984No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6030.11209449398537753No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCC5960.11079323120279437No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGA5940.11042144183634203No Hit
GTCCCTCACCTGCGATGTCTCTGACTACTCCATCACCAGTGGTTACAACT5870.1091201790537589No Hit
CTCCAGAGTCGAGTCACCATATCGCTAGACACGTCCAAGAACCAATTCTC5840.1085624950040804No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5810.1080048109544019No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTTGTGTCCCTTGGCCGAAGGTA5800.10781891627117572No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA5750.1068894428550449No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5740.10670354817181874No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5730.10651765348859256No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT5720.10633175880536641No Hit
GGACACAACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC5700.10595996943891409No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5680.10558818007246175No Hit
GTAGTAGGTGCTCCCACCATGAGCGCTACTCCCAATCCACTCCAGCCCCTTTCCTGGGGGCTGCCGGATCCAACC5640.10484460133955709No Hit
GAAATAAACGGCCGTGTCTGCGGCGGTGACAGAGTTCAGTTTCAGGGAGAATTGGTTCTTGGACGTGTCTAGCGA5620.10447281197310475No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGAGAAGGTGTCCCCCTTCTTCCAG5600.10410102260665242No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTAGAAATGCTTTAGGCTGGTA5570.10354333855697392No Hit
TTATTACTGTCTGCAAGATCACTATTACCTTACCTTCGGCCAAGGGACACAACTGGAGATTAAACGAACTG5550.10317154919052161No Hit
GGTAATAGTGATCTTGCAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAA5540.10298565450729544No Hit
CTCCAGTTGTGTCCCTTGGCCGAAGGTAAGGTAATAGTGATCTTGCAGACAGTAATAAGTTGCAAAATCTTCAGG5540.10298565450729544No Hit
CTCCATCACCAGTGGTTACAACTGGGGTTGGATCCGGCAGCCCCCAGGAAAGGGGCTGGAGTGGATTGGGAGTAG5460.10149849704148611No Hit
GCTTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTACAAAG5380.1000113395756768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA1303.6379788E-1271.2768770
ATCCAGG752.628892E-454.90958870
CTGGTAA200.007068683452.27557859
TCCGCCC200.00742332351.63244247
TGTTAGA3150.049.02641770
CTTATAC709.094947E-1248.6800041
CGTATGC704.674803E-1044.1031341
TCGTATG704.6929927E-1044.08662440
GTGGTAT950.043.0433731
GATGGTA508.821066E-442.9685269
GAGGAAA1752.4164729E-741.1821970
ACGAGAC759.804353E-1040.8988122
ATACACA857.6397555E-1140.0819634
GCTTGTG603.202822E-739.755341
TGAAGCG350.001432255438.9403845
TCTCGCG7150.038.87829270
TCTACAT901.364242E-1037.946932
TATACAC901.4006218E-1037.8551863
AAGGACA7100.037.70200770
CCGTCTT853.0304363E-936.4464347
Produced by FastQC (version 0.11.5)