FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537939
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15890.2953866516463763No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA12820.23831698389594358No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA12790.23775929984626512No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12760.23720161579658658No Hit
CTATTACCTTACCTTCGGCCAAGGGACACAACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTT11770.21879804215719625No Hit
GTCTGAGAGTCAGGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCA11350.2109904654616973No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC10170.18905489284100985No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9690.18013194804615393No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9690.18013194804615393No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9660.17957426399647541No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT9510.17678584374808295No Hit
GATCTATGCTGCATCCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCA9330.173439739450012No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG9310.17306795008355966No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG9060.16842058300290552No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9000.16730521490354855No Hit
GTCTCTGACTACTCCATCACCAGTGGTTACAACTGGGGTTGGATCCGGCAGCCCCCAGGAAAGGGGCTGGAGTG8850.1645167946551561No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC8710.16191426908998974No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC8540.15875405947514495No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCC8520.15838227010869263No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGT8470.1574527966925618No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGCCGGCGTAATTATTCCCCATACCTCTCGTACAGAAATAAACGGCCGTGT8460.15726690200933563No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8410.1563374285932048No Hit
AGGTAATAGTGATCTTGCAGACAGTAATAAGTTGCAAAATCTTCAGGCTG8390.1559656392267525No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8200.15243364024545536No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG8200.15243364024545536No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8190.15224774556222917No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8090.15038879872996752No Hit
ACTTATTACTGTCTGCAAGATCACTATTACCTTACCTTCGGCCAAGGGACACAACTGGAGATTAAACGAACTGTG7870.1462991156989919No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT7820.14536964228286106No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTAGAAATGCTTTAGGCTGGTATCAGCAGAAA7670.1425812220344686No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA7650.14220943266801625No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC7600.14127995925188544No Hit
CAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCC7580.14090816988543312No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG7550.1403504858357546No Hit
ACTACAACCCGTCCCTCCAGAGTCGAGTCACCATATCGCTAGACACGTCC7540.14016459115252844No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG7470.13886332836994528No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCCTAAAGCATTTCTAATGTCCTGACTT7410.1377479602705883No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7410.1377479602705883No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC6990.12994038357508936No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTGCAAGATCACTATTAC6950.1291968048421847No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6940.12901091015895855No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT6890.1280814367428277No Hit
GAGTGAAATCTGTGCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGTAAACTGGATGCAGCATA6790.12622248991056606No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC6700.12454943776153057No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTTGTGTCCCTTGGCCGAAGGTAAGGTAATAGT6680.12417764839507825No Hit
GTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGATTCTGTCCCAGGTGCG6460.12008796536410263No Hit
GGATTGGGAGTAGCGCTCATGGTGGGAGCACCTACTACAACCCGTCCCTCCAGAGTCGAGTCACCATATCGCTA6460.12008796536410263No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6450.11990207068087647No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG6400.11897259726474563No Hit
CAACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT6380.11860080789829329No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA6370.11841491321506713No Hit
AGTCAGGACATTAGAAATGCTTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTAT6330.11767133448216248No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG6330.11767133448216248No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC6270.11655596638280548No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT6230.11581238764990082No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT6080.11302396740150834No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG6030.11209449398537753No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6020.11190859930215137No Hit
GTACGGGGCTGTCTGAGAGTCAGGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTG6010.1117227046189252No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGTCAT5980.11116502056924671No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT5970.11097912588602053No Hit
ACTCTGGAGGGACGGGTTGTAGTAGGTGCTCCCACCATGAGCGCTACTCCCAATCCACTCCAGCCCCTTTCCTGG5960.11079323120279437No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG5940.11042144183634203No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA5880.10930607373698506No Hit
CTCCAGAGTCGAGTCACCATATCGCTAGACACGTCCAAGAACCAATTCTCCCTGAAACTGAACTCTGTCACCGCC5850.10874838968730656No Hit
GGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCGATGTCTCTGACTACTCCATCACCAGTGGTTACAA5820.10819070563762806No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTG5790.10763302158794956No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5780.1074471269047234No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT5750.1068894428550449No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5660.10521639070600942No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGT5530.10279975982406928No Hit
GGACACAACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC5530.10279975982406928No Hit
TTATTACTGTCTGCAAGATCACTATTACCTTACCTTCGGCCAAGGGACACAACTGGAGATTAAACGAACTGTG5510.10242797045761695No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5500.10224207577439078No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGC5490.10205618109116461No Hit
GTCAGGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGATT5490.10205618109116461No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA5490.10205618109116461No Hit
GAAATAAACGGCCGTGTCTGCGGCGGTGACAGAGTTCAGTTTCAGGGAGAATTGGTTCTTGGACGTGTCTAGC5430.10094081299180761No Hit
CTCCATCACCAGTGGTTACAACTGGGGTTGGATCCGGCAGCCCCCAGGAAAGGGGCTGGAGTGGATTGGGAGTA5410.1005690236253553No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5390.10019723425890296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAATT650.001994968971.9111670
AGACGGC150.002279535669.56779558
AATGGTA1604.5347406E-968.1657970
TTTAAGT1001.403721E-462.32370
AAAACCA1552.848592E-760.31258470
AAAGTCA800.004542643758.4278270
CGAATCA252.7388657E-454.47595630
GTGGTAT1300.052.273471
GACGGCA900.007239301751.9358470
ACCTTAG200.007722583651.11888532
GCTTATT200.00781419650.9666331
TAGACTG200.007851643550.905045
ACTTCGT200.007851643550.905048
TTGGTTT950.008964173549.2023770
GAGCCAT8350.046.6489670
GAGCAAA8800.046.03403570
GAGACCA1458.7843527E-442.98138470
CGTAATT7050.039.7806470
ACGCATC350.001454718438.8172720
CACGCAT350.001454718438.8172719
Produced by FastQC (version 0.11.5)