FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699880
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25030.3576327370406356No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT23380.3340572669600503No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC20720.29605075155740984No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16810.2401840315482654No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT16370.23389723952677602No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15380.21975195747842485No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15080.2154655083728639No Hit
CCTCATATATGGCGCTTCAACCCGGGCCACTGGTGTCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAAT14760.2108932959935989No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14650.20932159798822655No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14470.20674972852489No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13620.19460478939246728No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13420.19174715665542663No Hit
GTCTGTGTCTTCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCTGACTGTTTACACTAAGTTAGCCTGGTA13360.19088986683431444No Hit
AAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATATATGGCGCTTCAACCCGGGCCA13140.18774647082356977No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12840.1834600217180088No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC12560.17945933588615193No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT11760.16802880493798936No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGTTTCAAAGACCACTGCAGAAA11040.15774132708464308No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC10950.1564553923529748No Hit
CTCCTGCAGGGCCAGTCTGACTGTTTACACTAAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGC10850.1550265759844545No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC10800.15431216780019433No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10760.1537406412527862No Hit
GTCTGGGACTGAGGTGAAGAAGCCTGGGGCCTCGGTGAAGGTCTCCTGCAAGACTTCTGGATTCACCTTCACCGC10500.15002571869463338No Hit
GTCTGAAGACTTTGGAATTTATTACTGTCAGCAATACAGTAACTGGTCTC10400.14859690232611303No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10020.14316740012573584No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9720.1388809510201749No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT9710.13873806938332287No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9700.1385951877464708No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGACCAGTTACTGTAT9640.13773789792535862No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG9580.13688060810424643No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9340.13345144881979767No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9310.1330228039092416No Hit
GTCTCCGGCCACCCTGTCTGTGTCTTCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCTGACTGTTTACA9210.13159398754072127No Hit
GTAATATAAGGCGGTGTCGTCAGAGACCAGTCCTCTCAATTCCATGTAGGCCGTCTTCATGGCCGTGTCCCTGG9050.12930788135108875No Hit
CTGGTGTCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAATTCACT8840.1263073669771961No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8730.12473566897182374No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8530.1218780362347831No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8500.12144939132422701No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8480.12116362805052294No Hit
GTATTGCTGACAGTAATAAATTCCAAAGTCTTCAGACTGCAGACTGCTGATGTTGAGAGTGAATTCTGTCCCAGA8430.1204492198662628No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8420.12030633822941075No Hit
GTCTGGGACAGAATTCACTCTCAACATCAGCAGTCTGCAGTCTGAAGACTTTGGAATTTATTACTGTCAGCAATA8330.11902040349774247No Hit
ATACAGTAACTGGTCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA8330.11902040349774247No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA8310.11873464022403842No Hit
GTCTTCAGACTGCAGACTGCTGATGTTGAGAGTGAATTCTGTCCCAGACC8300.11859175858718637No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8250.11787735040292621No Hit
GGCCACTGGTGTCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAAT8210.11730582385551809No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGT8170.11673429730810997No Hit
CATATATGGCGCTTCAACCCGGGCCACTGGTGTCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAATTCA8120.1160198891238498No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA8100.11573412585014572No Hit
CCTATGCACAGGACTTTCAGGGGAGGGTCATCATGACCAGGGACACGGCC7920.11316225638680916No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7880.11259072983940105No Hit
GAATTCACTCTCAACATCAGCAGTCTGCAGTCTGAAGACTTTGGAATTTATTACTGTCAGCAATACAGTAACTGG7830.11187632165514089No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7790.11130479510773277No Hit
GTCTGACTGTTTACACTAAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATATATGGCG7710.11016174201291651No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGACACCAGTGGCCCGGGTTGAAGCGCCATATATGAGGAGCC7630.10901868891810024No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7590.10844716237069213No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7580.10830428073384007No Hit
AAGTAAGGGTACGCATTACTATTATATGTCGAGACAAATGCACAGTAATATAAGGCGGTGTCGTCAGAGACCAG7550.10787563582328398No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG7420.10601817454420757No Hit
CTGCTACTCTGGCTCCCAGTTTCAAAGACCACTGCAGAAATTCTGATGACGCAGTCTCCGGCCACCCTGTCTGTG7420.10601817454420757No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG7420.10601817454420757No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7400.1057324112705035No Hit
GGCTAACTTAGTGTAAACAGTCAGACTGGCCCTGCAGGAGAGGGTGGCTCCTTCCCCTGAAGACACAGACAGGG7330.10473223981253929No Hit
CCCTTACTTTGACCAGTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCT7300.1043035949019832No Hit
GTTACTGTATTGCTGACAGTAATAAATTCCAAAGTCTTCAGACTGCAGACTGCTGATGTTGAGAGTGAAT7240.103446305080871No Hit
CATCAGCAGTCTGCAGTCTGAAGACTTTGGAATTTATTACTGTCAGCAATACAGTAACTGGTCTCCGCTCACTT7230.10330342344401897No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7210.10301766017031491No Hit
CAGCAATACAGTAACTGGTCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCA7200.10287477853346287No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7190.10273189689661084No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC7130.10187460707549865No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7070.10101731725438647No Hit
TATATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAACTTAGTGTAAACAGTCAGACTGGCC7070.10101731725438647No Hit
GTGCATAGGTTGTGCCACCATTGCTGGGATTGATGTATCCCAGCCACTCCGGCCCCCGTCCAGGGGCCTGCCGCA7040.10058867234383037No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT7000.10001714579642225No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA752.898295E-453.84273570
GGGCGTA200.00795132150.7444464
GGTATCA5200.047.4915961
GTACGGA306.950237E-445.1061741
CTTATAC2050.044.55611
ATCTCGT1700.044.1708637
CGTATGC2050.043.45857241
TGTTAGA8150.043.35495870
GTGGTAT1250.043.301931
GTATGCC2150.043.07774742
TCGTATG1950.042.12404340
AGACCGA2100.041.9412425
TCTCCGA2200.041.51521711
TGCTTGA2150.040.69462255
ATCTCCG2250.040.59265510
ATGCCGT2300.038.85291344
TTATACA2700.038.8414272
TATACAC2450.038.6624373
GTATTCT350.001483879938.6624341
TGGTATC1400.038.6624342
Produced by FastQC (version 0.11.5)