FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699880
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23460.33520032005486655No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA23450.3350574384180145No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19370.2767617305823855No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC19010.2716179916557124No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16660.23804080699548494No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT16190.23132537006343945No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC15700.2243241698576899No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA14760.2108932959935989No Hit
CCTCATATATGGCGCTTCAACCCGGGCCACTGGTGTCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAATT14350.2050351488826656No Hit
AAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATATATGGCGCTTCAACCCGGGCCA13580.19403326284505917No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC13300.19003257701320225No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA12940.18488883808652912No Hit
GTCTGTGTCTTCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCTGACTGTTTACACTAAGTTAGCCTGGTA12890.18417442990226895No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12350.17645882151225922No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12260.17517288678059095No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12190.17417271532262674No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC12150.17360118877521863No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA10930.15616962907927073No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10420.1488826655998171No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10200.14573926958907243No Hit
CTCCTGCAGGGCCAGTCTGACTGTTTACACTAAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT10080.14402468994684803No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGACCA10000.14288163685203178No Hit
GTCTGAAGACTTTGGAATTTATTACTGTCAGCAATACAGTAACTGGTCTCCGCTCACTTTCGGCGGAGGGACCAA9950.14216722866777162No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC9900.14145282048351146No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT9840.14059553066239927No Hit
GTCTGGGACTGAGGTGAAGAAGCCTGGGGCCTCGGTGAAGGTCTCCTGCAAGACTTCTGGATTCACCTTCACCGC9710.13873806938332287No Hit
GTAATATAAGGCGGTGTCGTCAGAGACCAGTCCTCTCAATTCCATGTAGGCCGTCTTCATGGCCGTGTCCCTGGT9510.13588043664628222No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA9470.13530891009887408No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGACCAGTTACTGTATT9230.13187975081442532No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9170.13102246099331313No Hit
GTATTGCTGACAGTAATAAATTCCAAAGTCTTCAGACTGCAGACTGCTGATGTTGAGAGTGAATTCTGTCCCA9060.12945076298794078No Hit
GGCCACTGGTGTCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAATTCACTCTCAACATCAGCAGTCTGCA8940.1277361833457164No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8850.12645024861404813No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8810.12587872206664No Hit
GTCTCCGGCCACCCTGTCTGTGTCTTCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCTGACTGTTTACA8720.12459278733497171No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8700.12430702406126765No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG8680.12402126078756358No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8650.12359261587700748No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT8570.12244956278219123No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8480.12116362805052294No Hit
CTGGTGTCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAATTCACTCTCAACATCAGCAGTCTGCAGTCT8350.11930616677144654No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8320.11887752186089044No Hit
GAATTCACTCTCAACATCAGCAGTCTGCAGTCTGAAGACTTTGGAATTTA8090.11559124421329371No Hit
GTCTGACTGTTTACACTAAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATATATGGC8070.11530548093958964No Hit
ATACAGTAACTGGTCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT7920.11316225638680916No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7890.11273361147625308No Hit
GTCTGGGACAGAATTCACTCTCAACATCAGCAGTCTGCAGTCTGAAGACTTTGGAATTTATTACTGTCAGCAATA7830.11187632165514089No Hit
GTCTTCAGACTGCAGACTGCTGATGTTGAGAGTGAATTCTGTCCCAGACC7790.11130479510773277No Hit
CTGCTACTCTGGCTCCCAGTTTCAAAGACCACTGCAGAAATTCTGATGAC7590.10844716237069213No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT7570.10816139909698805No Hit
AAGTAAGGGTACGCATTACTATTATATGTCGAGACAAATGCACAGTAATATAAGGCGGTGTCGTCAGAGACCAGT7560.10801851746013602No Hit
CAGCAATACAGTAACTGGTCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCA7540.10773275418643195No Hit
GTGCATAGGTTGTGCCACCATTGCTGGGATTGATGTATCCCAGCCACTCCGGCCCCCGTCCAGGGGCCTGCCGCA7540.10773275418643195No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7480.10687546436531976No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7440.10630393781791164No Hit
CATATATGGCGCTTCAACCCGGGCCACTGGTGTCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAATTCA7300.1043035949019832No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7300.1043035949019832No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7220.10316054180716694No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7210.10301766017031491No Hit
CCCTTACTTTGACCAGTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTT7180.10258901525975882No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7080.10116019889123849No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7020.1003029090701263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTT150.0025640924203.6743370
GTGGTTA200.0060629696152.7557470
TAGATAT700.002896535765.4667470
CCGCTAT1051.9723106E-458.19266570
TTCTAGT800.004915626357.283470
GATCAGT800.004915626357.283470
AGAGCAA1706.194896E-753.9137970
GCTGATT250.00850244549.89007668
ATCCTAA1552.2663053E-549.27604770
GCGTGCA950.00969867948.23865570
TTTCAAA15250.047.0788270
GTGGTAT1750.046.2928661
GAGCAAA14200.040.87829670
ACTGCTA1500.00114514940.73486370
TGGTATC2100.038.5553442
AAGCGGT7300.037.665870
GGTATCA5550.037.100331
CTGTTAT908.329625E-636.9805869
GTATCAA13950.036.779881
ATTCGCA400.002478664734.82578352
Produced by FastQC (version 0.11.5)