FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453726
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGACTCTCAGACTGTTCATTTGTAAAAACA76950.5293294609850825No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG46410.31924860668379046No Hit
GTGTATTACTGTGCAACCGGGCGTCTCGACTCCCGCTATTATTTTGACTA40330.2774250443343519No Hit
GTATAAAAACTGGCCCCTGTGGACGTTCGGCCAGGGGACGAAGGTGGAAATCAAACGAACTGTGGCTGCACCA36760.2528674592048295No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT34710.23876576466266686No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG34250.23560148198491326No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA33610.23119900173760394No Hit
GTTTTTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAC31680.21792277224181172No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT30370.20891144548560045No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGG29790.2049216977614764No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29700.2043025989766985No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT28630.19694220231322823No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27210.18717419926451065No Hit
CTCCTGTGTAGCCTCTGGGCTCACCTTCGACACTTATTGGATGCACTGGGTCCGCCAAGCTCCAGGGAAGGGG26010.17891954880080566No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC25420.17486101232281737No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT23620.1624790366272599No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC22200.1527110335785423No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21310.14658883448462778No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC20960.14418122809938047No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20920.14390607308392367No Hit
CCTCATGTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGT20700.14239272049891108No Hit
CAGTAATACACAGCCGTGTCCTCGACTCTCAGACTGTTCATTTGTAAAAACAGAGTGCTCTTGGCGTTGTCTC20700.14239272049891108No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC20600.141704832960269No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTA19740.13578900012794706No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGTGGATGCACCATACATGAGGAGCC19650.1351699013431692No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTT19640.135101112589305No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG19480.13400049252747767No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT19370.1332438162349714No Hit
GTCTGAGAGTCGAGGACACGGCTGTGTATTACTGTGCAACCGGGCGTCTCGACTCCCGCTATTATTTTGACTA19320.13289987246565033No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19210.13214319617314405No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC19090.13131773112677356No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAAAAAC18790.1292540685108473No Hit
GTGTAGCCTCTGGGCTCACCTTCGACACTTATTGGATGCACTGGGTCCGCCAAGCTCCAGGGAAGGGGCTGGAG18470.12705282838719265No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTATCAGCAGAAACCTGGCCAGCCTCCCAGGC18440.12684646212560002No Hit
GCCCTGGGATTCCCAGCTGTTTCTGCTTGCTGATCAGGACTGCACACAGA18400.12657130711014317No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT18020.12395733446330327No Hit
GTCTGTGTCTCCAGGGGAAGGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTA17980.12368217944784643No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAGGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC17730.12196246060124122No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17700.12175609433964861No Hit
CTCTCAGACTGTTCATTTGTAAAAACAGAGTGCTCTTGGCGTTGTCTCTG17670.12154972807805597No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCC17630.12127457306259914No Hit
CCCTGAGGAGACGGTGACTAGGGTTCCCTGGCCCCAGTAGTCAAAATAAT17340.11927969920053709No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17330.1192109104466729No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC17300.11900454418508027No Hit
GGGCTGGAGTGGGTCGCACATGTTGATAGTTACGACTATACCAGAAACTT17230.11852302290803081No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC17110.11769755786166032No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTCGTCCCCTGGCCGAACGTCCACAGGGGCCAGTTTTTATAC16790.11549631773800566No Hit
GTGTGAGGTGCACCTGGAGGAATCCGGGGGAGGCTTAGTTCAACCTGGGGGGTCCCTGAGACTCTCCTGTGTAGC16700.11487721895322778No Hit
GTATTACTGTGCAACCGGGCGTCTCGACTCCCGCTATTATTTTGACTACTGGGGCCAGGGAACCCTAGTCACCG16510.11357023262980781No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC16420.11295113384502994No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGCCAGAGCATGTTCACCTGCCGCG16180.11130020375228894No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16150.11109383749069632No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16140.11102504873683211No Hit
CTCCAGCCCCTTCCCTGGAGCTTGGCGGACCCAGTGCATCCAATAAGTGT15690.10792955481294274No Hit
CCCTGAGACTCTCCTGTGTAGCCTCTGGGCTCACCTTCGACACTTATTGG15570.10710408976657225No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGCAGAAATAGTGA15510.10669135724338699No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCCGTGGTGACGCCAGACCCCACCTGCT15420.10607225845860911No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15340.10552194842769545No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCC15320.10538437091996704No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15220.10469648338132496No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCA15210.10462769462746076No Hit
GCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAG15050.10352707456563343No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC14950.10283918702699132No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC14920.1026328207653987No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT14910.1025640320115345No Hit
GACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACT14890.1024264545038061No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT14850.10215129948834925No Hit
GTGTTAGCACCAACTTAGCCTGGTATCAGCAGAAACCTGGCCAGCCTCCCAGGCTCCTCATGTATGGTGCATCCA14790.10173856696516399No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG14650.10077552441106509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG24200.066.17663670
CGTTTAT252.883368E-453.9179881
TAACCGG252.886309E-453.9068614
AATGGTA3200.052.19529770
CGTATGC2150.044.6815241
TCCGAGA16600.042.61444570
TCGTATG2100.042.41988840
GCGAATC2650.041.00176633
TGTTAGA8000.040.52811470
GACTAAG3050.039.86609326
TATACAC3350.039.2205773
AGACTAA3150.038.58985525
TAAGGCG3250.038.511829
CTTATAC3250.038.3647231
GCGTAGA20800.038.26080770
GTCACCG24700.038.18622670
ACGAGAC3150.037.4752822
ACTAAGG3250.037.441227
TTATACA3450.037.1059652
GGCGAAT3350.036.45312532
Produced by FastQC (version 0.11.5)