FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453726
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGACTCTCAGACTGTTCATTTGTAAAAACAGAGTGCTCTTGGCGTTGTCTCTGGA69350.47705000804828424No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAGGAAGATGCTGGCAAAGGATGG48520.3337630337491384No Hit
GTGTATTACTGTGCAACCGGGCGTCTCGACTCCCGCTATTATTTTGACTACTGGGGCCAGGGAACCCTAGTCACC37400.25726993945213883No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA35160.2418612585865562No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34550.2376651446008395No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34030.23408812939990065No Hit
GTATAAAAACTGGCCCCTGTGGACGTTCGGCCAGGGGACGAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT32040.22039916738092322No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA29740.2045777539921553No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGA29640.20388986645351326No Hit
GTTTTTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT29450.20258288013009332No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT29420.20237651386850064No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTC29110.20024406249871024No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG27280.1876557205415601No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25400.17472343481508895No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC24990.17190309590665642No Hit
CTCCTGTGTAGCCTCTGGGCTCACCTTCGACACTTATTGGATGCACTGGGTCCGCCAAGCTCCAGGGAAGGG23610.16241024787339567No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT21550.14823976457736876No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTCCAAGGACGTCATGCAGGGCACAGAC21290.14645125697689937No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC20350.1399851141136638No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20330.13984753660593538No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC19180.13193682991155142No Hit
CCTCATGTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTT19130.1315928861422304No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT19090.13131773112677356No Hit
GTCTGAGAGTCGAGGACACGGCTGTGTATTACTGTGCAACCGGGCGTCTCGACTCCCGCTATTATTTTGACTA18950.13035468857267463No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC18590.12787829343356313No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18350.12622736334082216No Hit
CTCTCAGACTGTTCATTTGTAAAAACAGAGTGCTCTTGGCGTTGTCTCTGGAGATGCTGAATCGGCCCTTGACG18340.12615857458695792No Hit
GGGCTGGAGTGGGTCGCACATGTTGATAGTTACGACTATACCAGAAACTT18020.12395733446330327No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17960.12354460194011802No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTATCAGCAGAAACCTGGCCAGCCTCCCAGGCT17750.12210003810896963No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGTGGATGCACCATACATGAGGAGCCTG17660.12148093932419177No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAAAAACTG17630.12127457306259914No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT17580.1209306292932781No Hit
CAGTAATACACAGCCGTGTCCTCGACTCTCAGACTGTTCATTTGTAAAAACAGAGTGCTCTTGGCGTTGTCTCTG17400.11969243172372235No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACACACATGGAGGACGCATTCTGCTGGAA17210.1183854454003024No Hit
GTCTGTGTCTCCAGGGGAAGGAGTCACCCTCTCCTGCAGGGCCAGTCAGA17200.1183166566464382No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC17050.11728482533847506No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGGA17040.11721603658461086No Hit
CCCTGAGGAGACGGTGACTAGGGTTCCCTGGCCCCAGTAGTCAAAATAAT16930.11645936029210456No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC16850.11590905026119089No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC16800.11556510649186987No Hit
GCCCTGGGATTCCCAGCTGTTTCTGCTTGCTGATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGA16710.11494600770709199No Hit
GTGTAGCCTCTGGGCTCACCTTCGACACTTATTGGATGCACTGGGTCCGCCAAGCTCCAGGGAAGGGGCTGGAGT16710.11494600770709199No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC16600.11418933141458569No Hit
CTCCAGCCCCTTCCCTGGAGCTTGGCGGACCCAGTGCATCCAATAAGTGTCGAAGGTGAGCCCAGAGGCTACACA16430.11301992259889415No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAGGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC16330.11233203506025206No Hit
GTGTGAGGTGCACCTGGAGGAATCCGGGGGAGGCTTAGTTCAACCTGGGGGGTCCCTGAGACTCTCCTGTGTAGC16280.11198809129093101No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC15530.1068289347511154No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGA15520.1067601459972512No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15200.10455890587359654No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA15150.1042149621042755No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT15090.10380222958109024No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAG15070.10366465207336184No Hit
GTATTACTGTGCAACCGGGCGTCTCGACTCCCGCTATTATTTTGACTACTGGGGCCAGGGAACCCTAGTCACCGT14980.10304555328858396No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTCGTCCCCTGGCCGAACGTCCACAGGGGCCAGTTTTTATACT14950.10283918702699132No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGCAGAAATAGT14860.10222008824221346No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA14830.10201372198062084No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGG14790.10173856696516399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA3800.069.0769870
TTCGGGT700.00277836266.1745870
CTAGTAC200.008092334550.5220223
TCGTGGT950.00930420648.7602270
TAAGTCA1305.376685E-447.50995670
GTAGTTT1305.376685E-447.50995670
GTGGCAT9200.046.99354670
GCAGTAT15600.046.5201770
AAAAGGT21000.045.58693770
TAGGCAA3851.8189894E-1244.1163970
TATGGTT2203.4103814E-642.111170
CGATTCA14300.039.95155770
AAACACA14900.039.37906370
GTATCAA24850.037.849121
GTGTCCA18800.037.78052570
GCAGCGT12450.035.96637370
GGTTGCA11950.034.88701670
CCTGCTT14200.034.79602470
AAAGTCA3151.0128751E-634.31274870
GTTTGGT2751.5764577E-533.68887770
Produced by FastQC (version 0.11.5)