FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326220
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT58830.44359156097781666No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50390.37995204415557No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA40940.30869689795056626No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT36500.275218289574882No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGCTTATCACTGGAGCTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAG35000.2639079489074211No Hit
CAATAATACACGGCCGTGTCCGCGGCAGTCACAGAGGTCAGCTTCAGGAAGAACTGGTTCTTAGACGTGTCTA34690.2615704785028125No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33640.25365324003558987No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG33370.2516173787154469No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33260.2507879537331665No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCG32590.24573600156836722No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA30520.23012773144727117No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29640.22349233158902745No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29550.2228137111489798No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC29480.22228589525116496No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGC29430.22190888389558294No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGATGAGGAGCC28720.21655532264631808No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28370.2139162431572439No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28040.2114279682104025No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27680.20871348645021187No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC26820.20222889113420098No Hit
GTATTATTGTGCGAGGGCCTATTACTATGATAGTAGTGGTTATTCCCCCGTGGGCTACTGGGGCCAGGGAACC26330.19853417984949706No Hit
ATAATACACGGCCGTGTCCGCGGCAGTCACAGAGGTCAGCTTCAGGAAGAACTGGTTCTTAGACGTGTCTACTGA26210.19762935259610018No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26030.19627211171600487No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC25950.19566889354707362No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25450.19189877999125335No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25020.1886564823332479No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23800.17945740525704634No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCACTCTT23450.17681832576797213No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCACTCTTTTCGGCCCTGGGACCAAAC23330.17591349851457527No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGA22870.17244499404322058No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT22540.1699567190963792No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA22450.16927809865633153No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT22060.1663374100827917No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21460.16181327381580732No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGA21250.1602298261223628No Hit
GTTTATTACTGTCAGCAGCGTAGCACTCTTTTCGGCCCTGGGACCAAACTGGATATCAAACGAACTGTGGCTGCA20870.1573645398199394No Hit
GTGCAGCCACAGTTCGTTTGATATCCAGTTTGGTCCCAGGGCCGAAAAGAGTGCTACGCTGCTGACAGTAATAAA20720.15623350575319328No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCAGTTTGGTCCCAGGGCCGAAAAGAGTGCTACGC19930.15027672633499722No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCAC18950.14288730376558942No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGCTTATCACTGGA18600.1402482242765152No Hit
GTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC18580.14009741973428239No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18480.13934339702311832No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18340.13828776522748865No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18140.13677971980516054No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18070.13625190390734568No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG18020.13587489255176366No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17960.13542247892506523No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC17780.13406523804496992No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17710.13353742214715508No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17640.13300960624934025No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA17440.1315015608270121No Hit
GTAATAGATGTACCCAATCCACTCCAGGCCCTTCCCTGGGTGCTGGCGGATCCAGCTCCAGTGATAAGCACCA17310.13052133130249882No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17210.12976730859133476No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17190.12961650404910197No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGT16830.12690202228891134No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16800.12667581547556211No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC16080.12124685195518088No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16070.12117144968406449No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC15990.12056823151513324No Hit
GTAATAGGCCCTCGCACAATAATACACGGCCGTGTCCGCGGCAGTCACAGAGGTCAGCTTCAGGAAGAACTGGT15750.1187585770083395No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15730.11860777246610668No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15710.11845696792387388No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15630.11785374975494263No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15310.11544087707921762No Hit
CTTTTCGGCCCTGGGACCAAACTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCA15300.1153654748081012No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA15200.11461145209693716No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15080.11370662484354029No Hit
GAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC15000.11310340667460904No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG14660.11053972945665123No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA14620.11023812037218561No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14430.1088054772209739No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14400.10857927040762469No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14340.10812685678092623No Hit
CTATTACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGACTTACCATATCAGTAGACACGTCTAAGAA14320.10797605223869342No Hit
CTGTAATAGATGTACCCAATCCACTCCAGGCCCTTCCCTGGGTGCTGGCGGATCCAGCTCCAGTGATAAGCACCA14190.10699582271418015No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14130.10654340908748171No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCACGGGGGAATAAC14070.10609099546078328No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT14050.10594019091855046No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14020.10571398410520125No Hit
GTGCTACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC13880.10465835230957156No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC13880.10465835230957156No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAC13870.10458295003845516No Hit
GCGTAGCACTCTTTTCGGCCCTGGGACCAAACTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT13830.10428134095398954No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG13780.10390432959840751No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13500.10179306600714814No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG13340.10058662966928565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8800.044.4624981
TCGTATG2350.043.76598440
AGTATCG405.334316E-542.65804734
AATGGTA3700.042.61250770
TGTTAGA11350.042.670
CTTATAC3700.041.37991
GTATCAA25050.041.0183261
TATACAC3850.040.64073
TTATACA3800.040.2788052
TATCTCG2750.039.75738536
CAATCTA350.001434187438.93389529
CCGATCA3500.038.91919728
ATCTCGT2850.038.3929237
CGTATGC2250.038.12304341
ACCGATC3500.037.94478627
TACACAT4050.037.7938845
GCCGTCT2350.036.63034446
AGACCGA3650.036.3730625
GTGGCCG17400.036.2451270
CACGAGA3950.036.18504721
Produced by FastQC (version 0.11.5)