FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658129
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATAAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG30950.4702725453520511No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG28570.43410942231690136No Hit
TATTATTACTGTGCAACATGGGATGACAGCCTGAGTGGTCGGGTGTTCGG26100.39657878622580073No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGACCACTCAGGCTGTCATCCCATGT24420.371051875848048No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG22590.3432457770437103No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20960.31847859614148594No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTG19500.296294495456058No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT17210.2614988854768594No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17110.2599794265258027No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGACCACTCAGG16440.24979905155372273No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCA16340.24827959260266605No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15170.23050192287530258No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14940.22700716728787212No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14690.22320851991023036No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13860.21059701061645972No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13160.19996079795906274No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12960.1969218800569493No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12720.19327517857441323No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12510.19008431477719412No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC12500.18993236888208848No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGGTGGTACCACTTTACAGTATTGCTTCCGA12480.18962847709187713No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCA12390.18826096403592607No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12330.18734928866529207No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG12310.18704539687508073No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11740.1783844808540575No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG11720.17808058906384616No Hit
CCCCTGAGGGCCGCTGATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGGTGGTACCACTTTA11700.17777669727363482No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC11680.17747280548342345No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11460.1741299957910987No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC11390.173066374525359No Hit
GTCTGAGGATGAGGCTTATTATTACTGTGCAACATGGGATGACAGCCTGAGTGGTCGGGTGTTCGGCGGAGGGAC11280.17139496967919662No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC11270.17124302378409095No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC11180.16987551072813992No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11080.16835605177708324No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG10930.16607686335049815No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAG10770.16364572902880742No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC10720.16288599955327906No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC10650.16182237828753937No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10180.15468092121757285No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10070.15300951637141047No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG10000.15194589510567078No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9890.1502744902595084No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT9740.14799530183292334No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTTATTATTACTGTGCAA9740.14799530183292334No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9700.14738751825250065No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA9540.14495638393080992No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9430.14328497908464755No Hit
TAATAAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAA9320.14161357423848514No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC9240.1403980070776398No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTTATTATTACTGTGCAACA9200.13979022349721712No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAC9190.13963827760211145No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGACCACTCAGGC8580.13036957800066554No Hit
GTATTGCTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT8550.12991374031534852No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8360.12702676830834075No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTGATTA8350.1268748224132351No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC8350.1268748224132351No Hit
GGCTTATTATTACTGTGCAACATGGGATGACAGCCTGAGTGGTCGGGTGTTCGGCGGAGGGACCAAGCTGACCG8290.12596314704260106No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8240.12520341756707273No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT8230.12505147167196703No Hit
ACATGGGATGACAGCCTGAGTGGTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG8220.12489952577686136No Hit
GTGCAACATGGGATGACAGCCTGAGTGGTCGGGTGTTCGGCGGAGGGACC8220.12489952577686136No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT8210.12474757988175571No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG8140.12368395861601601No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG8070.12262033735027632No Hit
GCTTGGTCCCTCCGCCGAACACCCGACCACTCAGGCTGTCATCCCATGTTGCACAGTAATAATAAGCCTCATCC8050.12231644556006498No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGG7980.12125282429432528No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7980.12125282429432528No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7890.11988531123837425No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACT7850.11927752765795156No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT7830.11897363586774021No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7770.11806196049710618No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7590.11532693438520412No Hit
GTTTGGGGGCCGTTCCTGGGAGGTGGTGGTACCACTTTACAGTATTGCTT7570.11502304259499278No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7480.11365552953904175No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7370.11198412469287936No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG7310.11107244932224533No Hit
GGTCTGGAGTGGGTCTCGCGTATTAGTTGGGATGGTGGTAGGAAAAACTA7270.11046466574182265No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA7240.11000882805650564No Hit
CTCAGGCTGTCATCCCATGTTGCACAGTAATAATAAGCCTCATCCTCAGA7190.10924909858097728No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGCAATACTGTAAAGTGGTACCACCACCTCCCAGGAACGGCCCC7050.10712185604949788No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7030.10681796425928655No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7010.10651407246907521No Hit
ATGTTGCACAGTAATAATAAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACT6990.10621018067886387No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6910.10499461351801852No Hit
GTATTGGGTGCTGCTTTTGATAGGTGGGGCCAAGGGACAATGGTCACCGT6820.10362710046206745No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6690.10165180382569375No Hit
GTATTACTGTGAAGTATTGGGTGCTGCTTTTGATAGGTGGGGCCAAGGGACAATGGTCACCGTCTCTTCCGCATC6670.10134791203548241No Hit
CTCCAGACCCTTCCCCGGAGCTTGACGGACCCACTGCATGTCATAATCAT6650.10104402024527107No Hit
GTCATCCCATGTTGCACAGTAATAATAAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGG6650.10104402024527107No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTGATTATTACTATAGATGAGGAGTT6650.10104402024527107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2050.0113.5156870
AATGGTA1550.075.0668270
GTATGTA208.9676556E-568.276291
GGAAACG600.006744983652.88798570
TGGAACG200.007636300351.2656832
GACAGGT1150.050.465081
TGGTATC1150.050.4344062
GCTAGTC352.4244859E-548.7465363
GTCGTAT652.2737368E-1047.45943539
TCGTATG2000.046.33307640
GTGGTAT1300.044.6421851
CGTATGC2150.043.11701641
ATGTCGT803.8198777E-1142.7930637
GCCGTCT2200.042.30204846
CACGAGA2550.041.48235321
GTATGCC2250.041.24485842
ATCTCCG2650.041.19523210
ATGCCGT2300.040.38532644
TGTCGTA857.094059E-1140.30962438
CGTCTTC2150.040.12566448
Produced by FastQC (version 0.11.5)