FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108034
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48570.4383439497343944No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT48260.43554620165085184No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTTATCGGGCCTCCGGGGTCTCTGACAGATTCAG40350.3641585005514271No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCCAGTCAGAGCCTCCTGCATAGTAATGGATACAACTTTT38200.34475476384298676No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT36300.3276072755890162No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33010.2979150459281935No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32520.2934927989574327No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31490.2841970553250171No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29890.26975706521641035No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28920.26100282121306745No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28560.2577538234386309No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTC28140.2539633260351217No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27200.2454798318463152No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC26530.23943308598833607No Hit
GCCCTGGCTTGTGCAGGAACCAATCCAAAAAGTTGTATCCATTACTATGC26440.23862083654472696No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25830.23311559031582063No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGG25310.22842259353052344No Hit
CTCCTTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCCAGTCAGAGCCTCCTGCATAGTAA25010.22571509538515966No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT24230.21867560020721385No Hit
GTAATGGATACAACTTTTTGGATTGGTTCCTGCACAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGT23880.21551685237095614No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22820.20595035892400412No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC21950.1980986143024492No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21080.19024686968089427No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20990.18943462023728513No Hit
GATTTTACACTGAAAATCAGCACCGTGGAGGCTGAGGATGTTGGAATTTATTACTGCATGCAAGCTCTGCAAAG20770.18744912159735172No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20690.18672712209192138No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG20560.18555387289559705No Hit
CTCCAGGGGTGACGGGCAAGGAGAGTGGAGACTGAGTCATCACAATATCCCCACTGGCTCCAGAGACCCAGAGCA20130.181673125553909No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGAGCCAGTGGGGATATTGTGATGA20070.18113162592483625No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGATCTCCAGCTTCGTCC18740.16912838414705686No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAATCTGTCAGAGACCCCGGAGGCCCGATAAGAACCCAAATAGA18700.1687673843943417No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18480.16678188575440825No Hit
GTCTCCACTCTCCTTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCCAGTCAGAGCCTCCTGCA17950.16199863903093226No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGAGCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCT17750.1601936402673564No Hit
GTGCAGCCACAGTTCGGTTGATCTCCAGCTTCGTCCCCTGGCCAAAACTC17710.15983264051464124No Hit
GTCTCTGACAGATTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCACCGTGGAGGCTGAGGA17620.1590203910710321No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17460.15757639206017143No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17440.15739589218381383No Hit
GCTAATGCTCTGGGTCTCTGGAGCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCTTGCCCGTCACCC17360.15667389267838353No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17320.15631289292566836No Hit
GTCAGAGACCCCGGAGGCCCGATAAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTGTGCAGGAA16750.15116864644947717No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16750.15116864644947717No Hit
GTGCAGGAACCAATCCAAAAAGTTGTATCCATTACTATGCAGGAGGCTCTGACTGGACCTGCAGGAGATGGAGGC16430.14828064842775582No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA16000.14439990108606776No Hit
GTCCGAGTTTTGGCCAGGGGACGAAGCTGGAGATCAACCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC15510.13997765411530694No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15450.1394361544862342No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG15330.13835315522808866No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15180.1369994061554068No Hit
ATCCATTACTATGCAGGAGGCTCTGACTGGACCTGCAGGAGATGGAGGCC15120.13645790652633405No Hit
GGCTTGTGCAGGAACCAATCCAAAAAGTTGTATCCATTACTATGCAGGAGGCTCTGACTGGACCTGCAGGAGA15050.13582615695908248No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA15000.13537490726818852No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14930.13474315770093698No Hit
GTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCACTGAATCT14850.13402115819550664No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGAGCCAGTGGGGATATTGTGATGACTCAGTCTCCAC14770.1332991586900763No Hit
GCATAGTAATGGATACAACTTTTTGGATTGGTTCCTGCACAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATT14650.13221615943193077No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14620.1319454096173944No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT14430.13023066079199735No Hit
GTTCCTGCACAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTTATCGGGCCTCCGGGGTCTCTGA14360.12959891122474582No Hit
GTAATATACGGCAGTGTCCTCAGCCTTTAGGCTGCTGATCTGCAGATATG14340.12941841134838822No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGAGCCAGTGGGGATAT14220.1283354120902427No Hit
AATCAGCACCGTGGAGGCTGAGGATGTTGGAATTTATTACTGCATGCAAG14120.1274329127084548No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14070.12698166301756084No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT13950.12589866375941533No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT13840.1249059144394486No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13810.12463516462491223No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCTTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGG13730.12391316511948189No Hit
AAGCTGGAGATCAACCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAA13460.1214764167886545No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT13400.12093491715958174No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13020.11750541950878765No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13010.11741516957060885No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12930.1166931700651785No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12900.11642242025064213No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12840.11588092062156938No Hit
GTAATAAATTCCAACATCCTCAGCCTCCACGGTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA12620.11389542198163595No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12590.11362467216709955No Hit
GGTCCAGTCAGAGCCTCCTGCATAGTAATGGATACAACTTTTTGGATTGGTTCCTGCACAAGCCAGGGCAGTCTC12580.11353442222892078No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12460.11245142297077526No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12400.11190992334170252No Hit
CCGTGGAGGCTGAGGATGTTGGAATTTATTACTGCATGCAAGCTCTGCAA12080.10902192531998114No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG11980.10811942593819324No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11970.10802917600001444No Hit
GCACAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTTATCGGGCCTCCGGGGTCTCTGACAGAT11940.10775842618547807No Hit
GGGTCTCTGGAGCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCTTGCCCGTCACCCCTGGAGAGCCGG11930.10766817624729927No Hit
ATTTGGGTTCTTATCGGGCCTCCGGGGTCTCTGACAGATTCAGTGGCAGT11890.10730717649458409No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11740.10595342742190221No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG11470.10351667909107483No Hit
CAGTAATAAATTCCAACATCCTCAGCCTCCACGGTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA11310.10207268008021414No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG11220.10126043063660502No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT11210.10117018069842622No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGAGCCAGTGGGGATATTGTGATGACTCAGTCTC11200.10107993076024742No Hit
ATGCAGGAGGCTCTGACTGGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAAGGAGAGTGGAGA11160.10071893100753225No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11140.10053843113117468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)