FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210214
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60020.49594534520341027No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA54420.44967253725374196No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATAAGGCGTCTA50760.41942995205806577No Hit
GTATAAAACTTATTCTTCGGCCACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCAT50160.41447215120631553No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATAAGGCGT46280.38241170569833105No Hit
GTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCATCAGGCTGCTGATGGTGAGAGTGAAGTCTGTC44760.3698519435405639No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39250.324322805718658No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT36770.30383056219809057No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC35550.2937497004661985No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC35140.29036186988416923No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC33450.2763973974850729No Hit
GTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGA31760.2624329250859765No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31670.26168925495821405No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30490.2519389132831053No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA30060.24838582267268433No Hit
GAGTGAAGTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATA28670.23690025069946305No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC28350.2342560902451963No Hit
GACTTCACTCTCACCATCAGCAGCCTGATGCCTGATGATTTTGCAACTTA27770.22946354942183778No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27330.22582782879722096No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA26920.22243999821519167No Hit
GGCCAAAAGTGGCCGAAGAATAAGTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCATCAGGCT26820.2216136980732333No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG26810.22153106805903752No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA26650.22020898783190412No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT26500.21896953761896656No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26470.21872164757637905No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG26000.21483803690917472No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGT25260.20872341585868284No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA24550.20285668485077848No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24540.20277405483658265No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGTGGCCGAAGAATAAGTTTTATACTGTT24470.20219564473721177No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT24260.20046041443909918No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT23510.19426316337441146No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAA23080.1907100727639905No Hit
GTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACA22730.1878180222671362No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCC22660.18723961216776538No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22360.18476071174189027No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGG22050.18219918130181934No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21850.1805465810179026No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA21780.17996817091853176No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTAATACTCTGACTGGCCCGGCAAGT21680.1791418707765734No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20590.17013519922922724No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19960.16492950833488954No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGACCTTA19740.16311164802258113No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19540.1614590477386644No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC18220.150551885864814No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18210.15046925585061816No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGAT17960.14840350549572223No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCA17820.14724668529698054No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17650.1458419750556513No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT17590.1453461949704763No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17340.1432804446155804No Hit
ACTTATTACTGCCAACAGTATAAAACTTATTCTTCGGCCACTTTTGGCCA17130.14154521431746783No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17050.14088417420390112No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16330.1349348131818009No Hit
CCTTATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTAATACTCT16260.13435640308243005No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAC15830.1308033124720091No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15610.1289854521597007No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG15540.12840704206032982No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15220.12576288160606305No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTAATACTCTGACTGGCCCG15190.12551499156347554No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA15150.1251844715066922No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCACT15140.12510184149249637No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC14980.12377976126536297No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14950.12353187122277548No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA14780.12212716098144626No Hit
GTAATAAGTTGCAAAATCATCAGGCATCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCC14750.12187927093885874No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14740.1217966409246629No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14720.12163138089627125No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14710.12154875088207541No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14540.12014404064074617No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCT14510.11989615059815868No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAG14320.11832618032843777No Hit
ATCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGATGCCTGATGATT14320.11832618032843777No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGA14280.11799566027165445No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14150.11692147008710856No Hit
CACCATCAGCAGCCTGATGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAAAACTTATTCTTCGGCCA14130.11675621005871688No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13910.11493834974640849No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA13760.11369889953347094No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTT13110.10832794861074158No Hit
GCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAACTA13110.10832794861074158No Hit
CCCCTAAGGTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTG13090.1081626885823499No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12590.10403118787255808No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT12540.10361803780157888No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12530.10353540778738304No Hit
TTATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTAATACTCTGACTG12440.10279173765962053No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12440.10279173765962053No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGATGCCTGATGATTTT12360.10213069754605383No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGA12330.10188280750346632No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGAT12310.10171754747507465No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTG12240.1011391373757038No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12170.10056072727633295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGG150.002521302667.8268924
AATGGTA6650.066.02408670
CTGATGT1052.8697168E-453.95516270
AGTAACG200.00787916950.86385323
TTGCAAT1256.7818194E-445.3223470
TAGGCAA3651.8189894E-1242.6837170
GTATCAA24150.039.99891
GAGCAAA32400.039.77944670
CTGCAGT8250.039.48536770
AACTGTA1808.578581E-539.34230870
CCTACTA5850.038.73703870
ATAAACG451.118837E-437.619333
GTCGGAA1550.001953500836.5502770
TATCAAC27000.035.4993062
ACTTCCA7600.035.40807370
CGTCACA13750.035.02180570
ATCAACG27550.034.4104273
TCAACGC28050.033.6763464
AACGCAG28650.033.4451646
CAACGCA28350.033.200555
Produced by FastQC (version 0.11.5)