FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566717
Sequences flagged as poor quality0
Sequence length18-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA70950.45285779116458175No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAACCACTCAGGCTGCTGTCATAGGA68400.4365817183320281No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG61070.389795987405511No Hit
CTCCTGCACTGGGAGCACCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAACACCTTCCAGGAAGAGC56670.36171178330228115No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAACCACTCAGG55430.35379714396409817No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG54550.3481803031434522No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG52350.33413820109183723No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC44470.28384194465241647No Hit
GTATATTACTGTGCGAAAGGAGGGCATTGTGGTGGTGACTGCTATTCGAGCCACTACGGTATGGACGTCTGGGGC39920.25480032450021284No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAG37770.24107736113158917No Hit
GGTTATGATGTACACTGGTACCAACACCTTCCAGGAAGAGCCCCCAAACTCCTCATCTACGGTAATGACAATCGG36980.23603496994032744No Hit
GATGTACACTGGTACCAACACCTTCCAGGAAGAGCCCCCAAACTCCTCAT36180.2309287510124675No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG35730.2280565028655462No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT34750.22180138467891775No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTT34440.219822724844372No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG34060.21739727085363855No Hit
CCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAACACCTTCCA33660.21484416138970855No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT33040.21088684172061706No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC32430.20699334978812384No Hit
GTCCTATGACAGCAGCCTGAGTGGTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG32210.20558913958296232No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG32090.20482320674378332No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31780.2028445469092376No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT31480.2009297148112901No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31220.1992701936597356No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTCGGTATTCGG30280.19327038641950015No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29930.19103641563856139No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTGTCA29840.19046196600917717No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29550.18861096164782792No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC29330.1872067514426664No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAACCACTCAGGC28720.1833132595101732No Hit
GTGTTGGTACCAGTGTACATCATAACCTGCCCCGATGTTGGAGGTGCTCCCAGTGCAGGAGATGGTGACCCTC27870.17788790189932197No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCACCTCCAACATCGGGGCAGGTTATGA27720.1769304858503482No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCACCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAA27700.17680283037715172No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC27420.17501565375240072No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT27240.17386675449363223No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC26990.17227106107867599No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26950.17201575013228299No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26710.17048388445392498No Hit
GTACCAACACCTTCCAGGAAGAGCCCCCAAACTCCTCATCTACGGTAATGACAATCGGCCCTCAGGGGTCCCTGA26030.166143598365244No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25620.16352666116471576No Hit
CTCCTGTGCAGCCTCTAGATTCACCTTTAGCAACTATGTCATGAGCTGGGTCCGCCAGGCTCCCGGGAAGGGG25070.16001613565181205No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG24790.15822895902706105No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG24640.1572715429780873No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT23900.15254829046981683No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT23450.14967604232289558No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT23130.1476335547517516No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC23040.14705910512236733No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTCGGTATTCGGCGGAGGGACCAAG22860.14591020586359885No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22700.14488896207802687No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG22040.14067633146254238No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21810.13920829352078262No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA21690.13844236068160362No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC21150.13499566290529813No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21110.13474035195890516No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21100.13467652422230691No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC21010.13410207459292264No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG20970.13384676364652964No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGCGACAGTCGGGGG20870.13320848628054716No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC20600.13148513739239442No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTCGGTAT20320.12969796076764342No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGG20280.12944264982125042No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC20280.12944264982125042No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC20070.12810226735268718No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19980.12752781772330293No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC19820.12650657393773093No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG19810.1264427462011327No Hit
GCCGAATACCGAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAAT19500.12446408636658694No Hit
ATACCGAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGA19320.12331518710781844No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGG19250.1228683929516307No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTA19240.12280456521503245No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG19160.12229394332224645No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18730.11954935064852172No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18530.11827279591655672No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC18340.11706006892118997No Hit
GCTTGGTCCCTCCGCCGAATACCGAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATCC18250.11648561929180573No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT18160.11591116966242149No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18130.11571968645262673No Hit
AGTACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACTCTGTATC18100.11552820324283199No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18080.1154005477696355No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18080.1154005477696355No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTG18020.115017581350046No Hit
GTATGTACTACCACCACTACCACTAATAGCTGAGACCCACTCCAGCCCCTTCCCGGGAGCCTGGCGGACCCAGC17960.1146346149304565No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17890.11418782077426874No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17810.11367719888148275No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCC17610.11240064414951775No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG17590.11227298867632127No Hit
GTGTACATCATAACCTGCCCCGATGTTGGAGGTGCTCCCAGTGCAGGAGA17570.11214533320312474No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG17320.11054963978816851No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG17250.11010284563198076No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17250.11010284563198076No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTG17070.10895394637321226No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC16980.10837949674382802No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTC16890.10780504711444377No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16780.10710294201186302No Hit
TCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC16690.10652849238247879No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTAGATTCACCTTTAGCAACTATGTCATGAGCTGGGTCCG16510.10537959312371029No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGAGTGTTCTTGGAATTGTCTC16470.10512428217731729No Hit
GTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC16430.10486897123092428No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC16220.10352858876236104No Hit
GGGCTGGAGTGGGTCTCAGCTATTAGTGGTAGTGGTGGTAGTACATACTA16190.1033371055525663No Hit
GCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCA16040.10237968950359254No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA15870.1012946179814223No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC15760.10059251287884155No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACCGTAG15730.1004010296690468No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15720.10033720193244856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG21150.075.0280870
AGGGGGG3050.061.20902670
GACCGGA1001.6809612E-551.85764770
GTGGTAT2300.048.906231
TGGTATC2450.045.903182
GGTATCA8800.044.931761
CGATTAA405.1620504E-542.8966541
ACGATTA405.180679E-542.8704740
AATGGTA3000.041.4861270
CTCTGGA20550.039.54982869
TATACAC2900.038.780273
TAGGCAA2957.2759576E-1238.67350470
GTATCAA21350.038.476651
AAAGCCA6200.038.4750370
CCTACGT459.901087E-538.40934451
CGTATGC2600.036.9571141
TCGTATG2600.036.93455540
CGCTAAT2600.036.78685432
CTTATAC2800.036.5208851
GCTTGTA1051.8189894E-1135.7093121
Produced by FastQC (version 0.11.5)