FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566717
Sequences flagged as poor quality0
Sequence length18-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGAGTGTTCTTGGAATTGTCTCTGGA61710.39388096254779903No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT59020.37671130140286985No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAACCACTCAGGCTGCTGTCATAG57230.3652861365517831No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCT54470.34766968125066616No Hit
CTCCTGCACTGGGAGCACCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAACACCTTCCAGGAAGAGC53890.3439676725279677No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG50250.32073437640620484No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG42730.27273591848432105No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAACCACTCAGG40800.26041716532085885No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT37820.24139649981458042No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC36180.2309287510124675No Hit
GTATATTACTGTGCGAAAGGAGGGCATTGTGGTGGTGACTGCTATTCGAGCCACTACGGTATGGACGTCTGGGGC36170.23086492327586924No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36120.23054578459287797No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG35520.226716120396983No Hit
GGTTATGATGTACACTGGTACCAACACCTTCCAGGAAGAGCCCCCAAACTCCTCATCTACGGTAATGACAATCG34600.220843968629944No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC33540.21407822855052955No Hit
GATGTACACTGGTACCAACACCTTCCAGGAAGAGCCCCCAAACTCCTCATCTACGGTAATGACAATCGGCCCTCA33240.21216339645258206No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT32820.20948263151545557No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA32540.2076954548907046No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT32280.20603593373915008No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG31780.2028445469092376No Hit
CCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAACACCTTCCA31710.20239775275304986No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA30930.19741918929838637No Hit
GTCCTATGACAGCAGCCTGAGTGGTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAG30790.19652560098601088No Hit
GTACCAACACCTTCCAGGAAGAGCCCCCAAACTCCTCATCTACGGTAATGACAATCGGCCCTCAGGGGTCCCT30650.19563201267363536No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30600.19531287399064415No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29070.18554723029111192No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29050.1854195748179154No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC28910.18452598650553992No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA28670.18299412082718194No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC28280.18050483909985018No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA27960.1784623515287062No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG27930.17827086831891145No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT27830.17763259095292896No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGG27390.174824170542606No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCACCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAA27070.172781682971462No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG26870.17150512823949698No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25760.16442024947709127No Hit
CTCCTGTGCAGCCTCTAGATTCACCTTTAGCAACTATGTCATGAGCTGGGTCCGCCAGGCTCCCGGGAAGGG25440.16237776190594727No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCACCTCCAACATCGGGGCAGGTTATGAT25190.16078206849099103No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT24610.15708005976829256No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTC24540.1566332656121048No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC24470.15618647145591705No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGG24440.1559949882461223No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC23790.15184618536723607No Hit
GTGTTGGTACCAGTGTACATCATAACCTGCCCCGATGTTGGAGGTGCTCCCAGTGCAGGAGATGGTGACCCTCT23740.15152704668424483No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT23180.14795269343474282No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAACCACTCAGGC22750.1452081007610181No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG22210.14176140298471263No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA21960.14016570956975638No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21940.1400380540965599No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTCGGTATTCGGCGGAGGGACCAA21660.1382508774718089No Hit
GGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC21150.13499566290529813No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT20540.1311021709728049No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT20500.1308468600264119No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGG20490.13078303228981367No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT20490.13078303228981367No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC20380.1300809271872329No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20070.12810226735268718No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGAT19970.1274639899867047No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG19960.12740016225010642No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC19910.1270810235671152No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19290.12312370389802371No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG19150.12223011558564821No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAC19130.12210246011245171No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAACCACTCA18620.11884724554594096No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG18540.11833662365315498No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGA18480.11795365723356546No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG18390.11737920760418123No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGG18260.11654944702840397No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18150.11584734192582324No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC18140.11578351418922497No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC18120.11565585871602849No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18100.11552820324283199No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGT18010.11495375361344774No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG17970.11469844266705474No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCACCT17690.11291126604230375No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC17530.11189002225673175No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGGCTGCGTCAGCACAGACTGGGCCCAG17440.1113155726273475No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC17390.11099643394435627No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCA17180.10965605147579302No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16980.10837949674382802No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAG16960.10825184127063153No Hit
GTATGTACTACCACCACTACCACTAATAGCTGAGACCCACTCCAGCCCCTTCCCGGGAGCCTGGCGGACCCAGCT16860.10761356390464902No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAG16830.10742208069485427No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCA16770.10703911427526477No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16490.10525193765051379No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA16470.10512428217731729No Hit
AGTACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACTCTGTATCT16250.10372007197215578No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC16190.1033371055525663No Hit
GCTTGGTCCCTCCGCCGAATACCGAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCT16090.1026988281865838No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT16080.10263500044998554No Hit
GCCGAATACCGAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAAT16080.10263500044998554No Hit
GGGCTGGAGTGGGTCTCAGCTATTAGTGGTAGTGGTGGTAGTACATACTA16070.1025711727133873No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16060.10250734497678905No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT16020.10225203403039604No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGG16010.1021882062937978No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTAGATTCACCTTTAGCAACTATGTCATGAGCTGGGTCC15990.10206055082060131No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAACGTGGTCGTCGCATGCCTGGTCCAGG15980.10199672308400305No Hit
GCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTCGGTATTCGGCG15960.10186906761080654No Hit
GGGTCACCATCTCCTGCACTGGGAGCACCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAACACCTTC15870.1012946179814223No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA15870.1012946179814223No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGT15790.10078399608863631No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC15790.10078399608863631No Hit
ATACCGAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGT15700.10020954645925206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3300.053.6257631
CGGCAAA900.007596231551.32878570
GGTTTGT900.007596231551.32878570
AAAGCCA6400.050.52676870
TGGTATC3500.050.5210882
TAAGTCA950.00940583648.62726670
GGTAGAA38350.046.57735470
GGTATCA10900.044.9593541
TCCTTTA1407.8522717E-443.99609870
CAGCCAT18800.041.7728970
GGTGCCA45800.041.35441270
AAGCGGT13400.040.22031470
TGACAGT27850.039.80975770
CTCTGGA19500.039.76329469
AATGGTA2854.7287904E-737.8212170
GCTTGTG1001.0913936E-1137.4349061
TTGTCAT21900.037.266170
GACAGGT2350.036.203971
GGTGAGT3007.0666283E-735.93014570
GCACCGT12600.035.441370
Produced by FastQC (version 0.11.5)