FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740731
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATCTTTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCA29510.3983902388316406No Hit
ATATTAATACTTGGTTGGCCTGGTATCAGCACAAACCAGGGAAAGCCCCTACCCTCCTGATCTATAAGGCGTCTA23800.32130422515056073No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT20320.2743236073554367No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19100.25785339077208863No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGGTGTCTCAACCCTGCCACTGGTG17200.23220305347015313No Hit
AGATTATACTGTTGACAGTAAAAAGTTGCAAAGTCATCAGGCTGCAGGTTGCTGATGGTGAGAGTGAATTCTGTC15820.21357280848243154No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT14110.19048750491068958No Hit
CAGTAATACAAGGCCGTGTCGTCAGATCGCAGCCTGCTGAGCTCCATGTAGCCTGCGCTGATGGACGTGTCCC13850.1869774587535826No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT13440.18144238596737547No Hit
GTGTATATAGTAGTCGGTAAAGGTGTATCCAGAAGTCTTGCAGGAGACCTTCACTGAGGCCCCAGGTTGCTTCAC13090.1767173238328084No Hit
GTATTAATATCGTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGATGCAGACAGGGTGGAAGGAGA12720.17172225814769465No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12510.1688872208669544No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12370.16699719601312757No Hit
GAATTCACTCTCACCATCAGCAACCTGCAGCCTGATGACTTTGCAACTTTTTACTGTCAACAGTATAATCTTTCC12310.1661871853614875No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11970.16159712500219378No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11910.1607871143505537No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11860.160112105474187No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCACGATATTAATACTTGGTTGGCCTGGTA11650.15727706819344675No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11400.15390202381161314No Hit
ACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGGTGTCTCAACCCTGCCACTGGTG11290.15241700428360633No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11280.152282002508333No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11140.15039197765450615No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10980.14823194925013264No Hit
GTAATACAAGGCCGTGTCGTCAGATCGCAGCCTGCTGAGCTCCATGTAGCCTGCGCTGATGGACGTGTCCCTGG10840.1463419243963058No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10700.14445189954247897No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10520.14202186758755878No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC10290.13891682675627184No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT10200.13770181077881175No Hit
GTATTACTGTGCGAGAGCCACCCCCCCATTTCAACACTGTACCAATGGTA10140.13689180012717167No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10020.13527177882389155No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCACGATATTAATACTTGG9650.13027671313877776No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC9290.12541664922893736No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9250.12487664212784398No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT9180.12393162970093057No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8870.11974657466745688No Hit
GTGTTGAAATGGGGGGGTGGCTCTCGCACAGTAATACAAGGCCGTGTCGTCAGATCGCAGCCTGCTGAGCTCCA8820.11907156579109016No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACA8250.11137646460050951No Hit
GTCTGGGGCTGAGGTGAAGCAACCTGGGGCCTCAGTGAAGGTCTCCTGCAAGACTTCTGGATACACCTTTACCGA8040.10854142731976926No Hit
ACTTTAGAAAGTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATTTGGGACAGAATTCACTCTCACCATCAGCAAC8000.10800142021867587No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7920.10692140601648913No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCC7890.10651640069066909No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG7840.10584139181430237No Hit
GTACCAATGGTATATGTACCCTTAAGGACTGGGGCCAGGGAGCCCTGATC7720.1042213705110222No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)