FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740731
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28780.38853510923668644No Hit
GTATAATCTTTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT25480.34398452339648267No Hit
ATATTAATACTTGGTTGGCCTGGTATCAGCACAAACCAGGGAAAGCCCCTACCCTCCTGATCTATAAGGCGTCTA22010.297138907376632No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20170.27229858072633656No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA18700.25245331976115487No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGGTGTCTCAACCCTGCCACTGGTGT15730.21235779250497144No Hit
AGATTATACTGTTGACAGTAAAAAGTTGCAAAGTCATCAGGCTGCAGGTTGCTGATGGTGAGAGTGAATTCTGTC14430.1948075617194366No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13200.1782023433608152No Hit
GTGTATATAGTAGTCGGTAAAGGTGTATCCAGAAGTCTTGCAGGAGACCTTCACTGAGGCCCCAGGTTGCTTCAC13180.1779323398102685No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT12950.17482729897898158No Hit
CAGTAATACAAGGCCGTGTCGTCAGATCGCAGCCTGCTGAGCTCCATGTAGCCTGCGCTGATGGACGTGTCCCTG12680.17118225104660126No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC11570.15619705399126No Hit
GAATTCACTCTCACCATCAGCAACCTGCAGCCTGATGACTTTGCAACTTTTTACTGTCAACAGTATAATCTTTCC11540.15579204866543994No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11130.1502569758792328No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11050.14917696167704605No Hit
GTATTAATATCGTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGATGCAGACAGGGTGGAAGGA11020.14877195635122603No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA10930.1475569403737659No Hit
ACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG10890.14701693327267254No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCACG10880.1468819314973992No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10330.13945683385736524No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC10260.13851182143045182No Hit
GTAATACAAGGCCGTGTCGTCAGATCGCAGCCTGCTGAGCTCCATGTAGCCTGCGCTGATGGACGTGTCCCTGGT10030.1354067805991649No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC9820.13257174331842464No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9730.1313567273409645No Hit
GTATTACTGTGCGAGAGCCACCCCCCCATTTCAACACTGTACCAATGGTATATGTACCCTTAAGGACTGGGGCCA9600.12960170426241105No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA9460.12771167940858422No Hit
GTCTGGGGCTGAGGTGAAGCAACCTGGGGCCTCAGTGAAGGTCTCCTGCAAGACTTCTGGATACACCTTTACC9140.1233916225998372No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG8900.12015157999327691No Hit
GAGTGAATTCTGTCCCAAATCCACTGCCGCTGAACCTTGACGGGACCCCACTTTCTAAAGTAGACGCCTTATA8870.11974657466745688No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG8440.11394149833070305No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8430.1138064965554297No Hit
GTGTTGAAATGGGGGGGTGGCTCTCGCACAGTAATACAAGGCCGTGTCGTCAGATCGCAGCCTGCTGAGCTCCAT8410.11353649300488301No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC8260.11151146637578284No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8210.11083645749941612No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT8190.11056645394886944No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGAAA8120.10962144152195602No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8060.10881143087031594No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7940.10719140956703581No Hit
GTACCAATGGTATATGTACCCTTAAGGACTGGGGCCAGGGAGCCCTGATCACCGTCTCCTCAGCATCCCCGACCA7930.10705640779176245No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7800.10530138471320898No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7770.10489637938738894No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGT7620.10287135275828876No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG7570.10219634388192204No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA7560.1020613421066487No Hit
CCATGTAGCCTGCGCTGATGGACGTGTCCCTGGTCATGGTGACCCTGCCCTGAAATTTCTGTGCATAGTTTGT7450.10057632257864191No Hit
ACTTTAGAAAGTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATTTGGGACAGAATTCACTCTCACCATCAGCAAC7450.10057632257864191No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT7430.10030631902809523No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7410.10003631547754854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)