FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601779
Sequences flagged as poor quality0
Sequence length18-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132942.2091166358413967No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTA25080.4167642938686793No Hit
GTACCTATACTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATTTGTTTCAGCTATTACTAGTGATG17940.2981160858055864No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT17770.2952911284707509No Hit
GTAATACACAGCCATGTCCTCAACTCTCAGGCTGCCCATTTGAAGAAACAGCGTGTTCTTGGAGTTGTCTCTGGA17490.29063825756631584No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC17380.2888103439967164No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16690.27734434069650155No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGA14690.2441095485219657No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACACCATTCAGGCTGTCATCCCAT13950.23181267541738745No Hit
GTGTATTACTGTGCGAGGAGGAATAGTGGGAGCTACTACGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC13520.22466719509986222No Hit
GTATAGGTACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCCCCCAGGCTGGACCAAGCCTCCC12520.20804979901259432No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT12470.20721892920823093No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG11230.18661335806001872No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11150.18528396637303726No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT10750.17863700793813012No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC10430.17331944119020437No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCA10260.17049448385536883No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCA10240.17016213593362348No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA9680.16085639412475344No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC9570.15902848055515398No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9530.15836378471166324No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA9460.15720056698555449No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACACCATTCAGG9150.15204917419850145No Hit
CATCTATAGTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC9000.14955656478541124No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8990.1493903908245386No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8830.1467316074505757No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTAAAGTATTACTTCCGATGTTGGA8540.141912562585268No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG8470.14074934485915927No Hit
CAGTAATACACAGCCATGTCCTCAACTCTCAGGCTGCCCATTTGAAGAAACAGCGTGTTCTTGGAGTTGTCTCTG8210.1364288218764696No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8190.13609647395472424No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT8120.1349332562286155No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG7970.1324406468155253No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7930.13177595097203457No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG7900.13127742908941653No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGG7790.1294495155198171No Hit
GTATTACTGTGCGAGGAGGAATAGTGGGAGCTACTACGACTACTGGGGCC7760.12895099363719906No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7730.12845247175458102No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTACCTATACTATGCACTGGGTCCGCCAGGCTCCAGGGAAGG7690.12778777591109028No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG7640.12695690610672689No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGG7640.12695690610672689No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAC7620.12662455818498153No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTTTTCGGCGGAGGGAC7520.12496281857625473No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7470.12413194877189135No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTTTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCC7360.12230403520229187No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA7290.1211408174761831No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG7210.11981142578920169No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT7080.11765116429785685No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC7070.11748499033698417No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC7030.11682029449349347No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG7010.1164879465717481No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA6990.11615559865000274No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT6950.11549090280651203No Hit
GCATGGGATGACAGCCTGAATGGTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAG6950.11549090280651203No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT6890.11449385904127596No Hit
CCCCTGAGGGCCGCTGATTATCACTATAGATGAGGAGTTTGGGGGCCGTT6790.11283211943254916No Hit
GCACTGAACACAGAAGAGTCATGACGGAGTTTGGGCTGAGCTGGGTTTTC6730.11183507566731307No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC6340.1053542911932786No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6300.10468959534978786No Hit
GATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTAAAGTATTACTTCC6280.10435724742804253No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG6270.10419107346716983No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGAT6230.10352637762367912No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6220.10336020366280645No Hit
ACTTTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTGATAATCAGCGGCCCTCA6210.10319402970193375No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT6150.10219698593669768No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG6120.10169846405407965No Hit
GTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG6040.10036907236709823No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA6020.10003672444535286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAA355.656166E-7189.1100870
CATCCTT750.00277184966.1885370
TCGGCAA351.2009035E-464.3562769
AAGCGGT5250.050.42935670
GAACTCG304.2294041E-449.88553265
CGCTCCG200.00868651849.6207735
GAGCCAT6450.046.17804370
CTTAGAC307.7598065E-444.1073533
AACTCGG358.382699E-443.42006366
CTCTTGT15500.041.6347270
GGTAGAA10750.041.56023870
GCTTGTA406.373861E-541.3850331
AGTGGAA6250.039.7131270
ACAGGTT350.001649514837.8377381
TGGTATC1750.037.809442
GGTATCA5600.037.2465251
TATAAGA451.2812206E-436.7591822
GTGGTAT1900.036.593081
TGACAGT15400.035.4581470
AGTTTAA8000.035.16265570
Produced by FastQC (version 0.11.5)