FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774210
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT40090.5178181630307023No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38980.5034809676961031No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27570.35610493277017863No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT26820.3464176386251792No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25080.3239431162087805No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24450.3158057891269811No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23330.3013394298704486No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23230.3000477906511153No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23130.2987561514317821No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22630.29229795533511577No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC22190.28661474277004945No Hit
CTACAAACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACG22170.2863564149261828No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTATATAGTAATGGATACAACTATT21690.2801565466733832No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20750.2680151380116506No Hit
GAGTTTGTAGAGATTGCATGCAGTAATAAACGCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAA20140.2601361387737177No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTC19680.25419459836478475No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGCGTTTATTACTGCATGCAATCTCTACAAAC19650.25380710659898476No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT18450.2383074359669857No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC18180.23482001007478592No Hit
CCTATATAGTAATGGATACAACTATTTGGAATGGTATCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTA17030.21996615905245345No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16880.21802870022345358No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAATGGAGTTTG16270.21014970098552072No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG15890.20524147195205436No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15710.2029165213572545No Hit
GGTCTAGTCAGAGCCTCCTATATAGTAATGGATACAACTATTTGGAATGGTATCTGCAGAAGCCAGGGCAGTCTC15450.19955825938698804No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15440.19942909546505472No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGG15260.19710414487025485No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGA14970.1933583911341884No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14860.19193758799292182No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13940.18005450717505586No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCTAGTGGGGATATTGTGATGA13860.17902119579958925No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13810.17837537618992264No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13580.17540460598545615No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGAGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAG13500.17437129460998954No Hit
CTCCTATATAGTAATGGATACAACTATTTGGAATGGTATCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGA13470.17398380284418957No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12990.16778393459138993No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12870.16623396752819003No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACTCAAATAGA12760.16481316438692342No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGCGTTTA12640.16326319732372355No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12580.16248821379212358No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12120.1565466733831906No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA12070.15590085377352397No Hit
ATATAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA11890.15357590317872413No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT11850.1530592474909908No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA11630.15021764120845765No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCTAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC11430.14763436276979114No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG11370.14685937923819117No Hit
GCTAATGCTCTGGGTCTCTGGATCTAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC11320.14621355962852456No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11230.14505108433112462No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACTCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGATA11200.14466359256532466No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCCCCACTAGATCCAGAGACCCAGAGCA11130.14375944511179137No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10970.14169282236085814No Hit
GTAATACACGGCAGTGTCCTCAGCCTTTAGGGTGTTGATCTGCAGATATG10910.1409178388292582No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG10840.14001369137572492No Hit
GAGTGGAGGCTGAGGATGTTGGCGTTTATTACTGCATGCAATCTCTACAAACTCCATTCACTTTCGGCCCTGGGA10780.13923870784412498No Hit
CTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC10740.1387220521563917No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10740.1387220521563917No Hit
GTGGAGGCTGAGGATGTTGGCGTTTATTACTGCATGCAATCTCTACAAAC10690.13807623254672505No Hit
GTAATAAACGCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA10680.13794706862479172No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTATA10630.1373012490151251No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10570.13652626548352514No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10450.13497629842032524No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10340.13355549527905866No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10260.13252218390359205No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAA10190.13161803645005876No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCTAGTGGGGATATTGTGATGACTCAGTCTCCAC9990.12903475801139228No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9970.1287764301675256No Hit
TTATTACTGCATGCAATCTCTACAAACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTG9910.12800144663592564No Hit
GTATCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGAGTTCTAATCGGGCCTCCGGGGTCCCTGA9850.1272264631043257No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9780.1263223156507924No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9760.12606398780692576No Hit
GTGAATGGAGTTTGTAGAGATTGCATGCAGTAATAAACGCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAG9680.12503067643145918No Hit
ATATAGTAATGGATACAACTATTTGGAATGGTATCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATT9620.12425569289985922No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9490.12257656191472596No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTATATAGTAA9450.12205990622699268No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT9370.12102659485152607No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9260.1196057917102595No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9240.11934746386639283No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9210.11895997210059285No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9180.1185724803347929No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT9130.11792666072512625No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCTAGTGGGGATAT9090.11741000503739296No Hit
GGATACAACTATTTGGAATGGTATCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGAGTTCTAA9020.11650585758385967No Hit
CAGTAATAAACGCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA8980.11598920189612638No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8920.11521421836452643No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG8920.11521421836452643No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8920.11521421836452643No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8730.11276010384779324No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA8690.11224344816005993No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGCGTTTATTACTGCATGCAATCTCTA8570.11069348109686002No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT8400.10849769442399348No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8340.10772271089239355No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8260.10668939951692692No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8180.10565608814146032No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8140.10513943245372703No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8100.10462277676599371No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8040.10384779323439376No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTT7860.10152284263959391No Hit
GTTTATTACTGCATGCAATCTCTACAAACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA7770.10036036734219397No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCTA200.001149761782.661669
CGGAATT150.00232691469.2108248
TGTTAGA10800.060.53144570
GGTATCA8250.047.547381
ATGGCAA1351.8228111E-646.11919470
GTCCTAT452.1356518E-645.088041
ACGAGAC1500.040.6002222
AGACGTA350.00139257539.1657241
CGGGATC853.61797E-935.7959517
GTATCAA19650.035.622991
TAAGTCA1751.08411095E-535.57766770
GAGCAAA15200.035.49964570
TCGTATG1350.035.49510240
GTGGTAT2050.034.6408041
GTGTTAG10900.033.87355869
CACGAGA1800.033.82258621
CCCACGA1800.033.8138419
TCCGAGC1800.033.8007413
TACGTAC701.1426E-633.79855312
TCTCCGA1800.033.7963711
Produced by FastQC (version 0.11.5)