FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774210
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38240.49392283747303706No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA38210.49353534570723706No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28760.37147543948024436No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT25610.33078880407124683No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC23280.300693610260782No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA22730.29358959455444905No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22550.2912646439596492No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21500.27770243215664997No Hit
CTACAAACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACG21190.27369835057671693No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20190.26078195838338436No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA19800.2557445654279847No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTATATAGTAATGGATACAACTATTTG19610.25329045091125146No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC19470.25148215600418494No Hit
GAGTTTGTAGAGATTGCATGCAGTAATAAACGCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT19290.24915720540938505No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTG19270.2488988775655184No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGCGTTTATTACTGCATGCAATCTCTACAAACT18630.24063238656178557No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18510.23908241949858566No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18440.23817827204505237No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA17700.22862014182198626No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT16820.21725371669185362No Hit
CCTATATAGTAATGGATACAACTATTTGGAATGGTATCTGCAGAAGCCAG16800.21699538884798697No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16290.2104080288293874No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15780.20382066881078778No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15780.20382066881078778No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG15140.19555417780705492No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA15050.194391702509655No Hit
CTCCTATATAGTAATGGATACAACTATTTGGAATGGTATCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCT15040.1942625385877217No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC14520.18754601464718873No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCTA14470.18690019503752212No Hit
GGTCTAGTCAGAGCCTCCTATATAGTAATGGATACAACTATTTGGAATGGTATCTGCAGAAGCCAGGGCAGTCTC14460.1867710311155888No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14050.18147531031632244No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT13810.17837537618992264No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGAT13580.17540460598545615No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13540.17488795029772286No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCTAGTGGGGATATTGTGAT12950.16726727890365664No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACTCAAATA12890.16649229537205668No Hit
ATATAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGA12480.16119657457279032No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGAGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTG12400.1601632631973237No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12310.1590007878999238No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG11950.15435088671032407No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11780.15215510003745755No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT11750.15176760827165758No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT11310.14608439570659124No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC11210.14479275648725798No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11200.14466359256532466No Hit
GAGTGGAGGCTGAGGATGTTGGCGTTTATTACTGCATGCAATCTCTACAAACTCCATTCACTTTCGGCCCTGG11140.1438886090337247No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC11020.1423386419705248No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10820.13975536353185827No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10810.13962619960992495No Hit
GCTAATGCTCTGGGTCTCTGGATCTAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC10720.13846372431252502No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10720.13846372431252502No Hit
CTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC10580.13665542940545847No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10520.13588044587385853No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG10480.1353637901861252No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA10400.1343304788106586No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10370.13394298704485863No Hit
GTAATAAACGCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA10370.13394298704485863No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCTAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC10310.13316800351325866No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA10310.13316800351325866No Hit
GTGGAGGCTGAGGATGTTGGCGTTTATTACTGCATGCAATCTCTACAAAC10280.1327805117474587No Hit
TTATTACTGCATGCAATCTCTACAAACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGT10230.13213469213779208No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG10080.13019723330879218No Hit
ATATAGTAATGGATACAACTATTTGGAATGGTATCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTT9990.12903475801139228No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9960.12864726624559228No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTATATAGTAATG9860.12735562702625902No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9860.12735562702625902No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACTCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGATA9800.12658064349465906No Hit
GTATCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGAGTTCTAATCGGGCCTCCGGGGTCCCT9670.12490151250952584No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9630.12438485682179252No Hit
GTGAATGGAGTTTGTAGAGATTGCATGCAGTAATAAACGCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGT9570.12360987329019259No Hit
GTAATACACGGCAGTGTCCTCAGCCTTTAGGGTGTTGATCTGCAGATATGCCGTGCTGATAGAGGTGTCCAAGGA9530.12309321760245928No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9190.1187016442567262No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCTAGTGGGGATATTGTGATGACTCAGTCTCCACT9010.11637669366192636No Hit
CAGTAATAAACGCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA9000.11624752973999303No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8990.11611836581805969No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT8800.11366425130132651No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA8740.11288926776972655No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCTAGTGGG8680.1121142842381266No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG8480.10953100579946011No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8430.10888518618979345No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8330.10759354697046021No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTG8270.10681856343886026No Hit
GGATACAACTATTTGGAATGGTATCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGAGTTCTAAT8210.1060435799072603No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT8200.10591441598532698No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGCGTTTATTACTGCATGCAATCTCTA8160.10539776029759368No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8160.10539776029759368No Hit
GTTTATTACTGCATGCAATCTCTACAAACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA8090.1044936128440604No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTT8070.10423528500019374No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7800.10074785910799396No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGA7760.10023120342026065No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAA550.001338630979.53451570
AGAGCAT8400.065.9631570
TAGGCAA2900.060.33652570
ATGGCAA1258.358158E-658.32530670
GTGGTAT2400.045.0029341
GTATCAA20700.041.0896341
GGTATCA7150.040.1249921
GCGGGAA18350.038.1418970
GAGCAAA22100.036.9481670
GGAGCTT1600.001977126336.45331670
ATCAACG23700.035.8466953
TATCAAC24100.035.118642
TCAACGC24100.035.114114
GAGCCAT5850.034.8954870
AACGCAG24450.034.611456
CAACGCA24500.034.5408175
TATAAGA1203.6379788E-1233.7194942
TCTTGTT4456.548362E-1032.7670370
AGAGTAC26600.032.3229911
ACGCAGA26250.032.2380947
Produced by FastQC (version 0.11.5)