FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298587
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTA83080.6397723063606828No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC63760.49099521248865113No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT60310.4644278742972169No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGA58720.45218379669594727No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT53950.4154515638921381No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG50310.38742109693074084No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACACCATTCAGGCTGTCATCCCAT50050.3854189207192125No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG39570.30471581803914566No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37560.289237455788484No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC37500.28877541512428506No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37450.2883903812374527No Hit
GTAATAGACAGCCATGTCCTCAACTCTCAGGCTGCCCATTTGAAGATACAGCGTGTTCTTGGAGTTGTCTCTGGA36790.28330793393126524No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACACCATTCAGG35010.26960072756003256No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG33830.2605139278307884No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT33670.2592818193929248No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC33390.25712562962666347No Hit
CATCTATAGTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC32900.2533522975357061No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32690.25173515521101014No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA31590.24326440970069774No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA30330.2335615557525218No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTAAAGTATTACTTCCGATGTTGGA30040.23132835920889397No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29970.23078931176732867No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG29940.2305582914352292No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28540.21977734260392257No Hit
GTCTATTACTGTGCGAGGAGGAATAGTGGGCATTACTACGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC27940.21515693596193403No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAC26930.20737925144791994No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCA26490.203990953243795No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGG26310.2026048312511984No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCGCTCACTGTGCAGGGTCCTGGGCCCAGT25210.19413408574088606No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA24900.1917468756425253No Hit
GCATGGGATGACAGCCTGAATGGTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAG24810.191053814646227No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTTTTCGGCGGAGGGAC24730.1904377604272952No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGG24650.18982170620836342No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24590.18935966554416453No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT24550.18905163843469863No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG24540.18897463165733216No Hit
CCCCTGAGGGCCGCTGATTATCACTATAGATGAGGAGTTTGGGGGCCGTT24210.18643340800423844No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTTTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCC23290.17934878448652267No Hit
GATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTAAAGTATTACTTCC23050.17750062182972723No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT22600.1740353168482358No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCGCTCA22160.17064701864411086No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT21980.1692608966515143No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC21950.16902987631941488No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG21750.16748974077208534No Hit
GTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG20880.16079015114120193No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGAT20880.16079015114120193No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG20830.16040511725436954No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20500.15786389360127584No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT19900.15324348695928727No Hit
ACTTTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTGATAATCAGCGGCCCTCA19850.1528584530724549No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19770.15224239885352311No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19630.15116430397039243No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGT19450.14977818197779588No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG19320.14877709387203167No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC18940.14585083633210558No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC18700.14400267367531017No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC18540.14277056523744655No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCGCTCACTGTGCAGGGTCCTGGGC18510.14253954490534712No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTC18430.1419234906864153No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACACCATTCAGGC18400.14169247035431587No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCGCTCACTGTGCAGGGT18290.14084539580328465No Hit
CCTCGCTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCA17970.1383811789275574No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG17800.13707206371232733No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17640.13583995527446371No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATG17290.13314471806663705No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACACCATTCA17160.13214362996087284No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC17110.13175859607404047No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTACCTATGATATCCACTGGGTCCGCCAGGCTCCAGGGAAGG17050.13129655540984161No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCA16750.12898635208884734No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTG16750.12898635208884734No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16720.12875533175674791No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAG16690.12852431142464849No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTAAAGTATTACTT16640.12813927753781612No Hit
GTGCAGCATGGGATGACAGCCTGAATGGTGTGGTTTTCGGCGGAGGGACC16420.12644512843575362No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAGTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG16330.12575206743945536No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16320.1256750606620889No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAG15980.12305683023162868No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGG15980.12305683023162868No Hit
GTTTAAAGTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCA15960.12290281667689575No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT15850.12205574212586448No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC15460.11905247780857195No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC15370.11835941681227365No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15250.11743533548387594No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCA15150.11666526771021118No Hit
GAAGTAATACTTTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTGATAATCAGC15150.11666526771021118No Hit
GGATGACAGCCTGAATGGTGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC15040.11581819315917995No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTTTAAACTGGTACCAGCAGCTCCCAG14930.11497111860814871No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC14360.11058173229825957No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA14200.10934962386039596No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAG14170.10911860352829653No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC14120.10873356964146415No Hit
GCCCCCAAACTCCTCATCTATAGTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT13910.10711642731676815No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTGATTATCACTATAGATGAGGAGTTTG13850.1066543866525693No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC13550.10434418333157501No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13490.10388214266737615No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13430.10342010200317729No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTTTAAA13370.10295806133897845No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGG13330.10265003422951255No Hit
CTCCTCATCTATAGTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCA13140.10118690545954949No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC13060.10057085124061768No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAA650.002367769868.8971670
ACTTATC251.6897405E-460.07476467
CCCCATA750.00417454259.71087670
AATGGTA2601.8189894E-1257.41430370
TGCCAAT1102.7853253E-454.28261670
GGTAGAA37450.054.2101470
AGTTTGT850.0068504352.68606670
CGCGCTA200.00786237950.891526
TCGCGCT200.007863576550.8895655
CCTCAAA900.00858719749.75906470
GGTATCA8700.047.2158661
AAGCGGT8450.044.1648570
CTCTTGT47200.043.96086570
TGGATAT6250.042.99183370
GTGGTAT1900.039.3089031
CAGAACA17950.039.08650670
GCGCTAC350.001463386738.774497
GTATCAA19500.037.4305041
AGTGGAA21150.037.40752470
TCACCAT53800.035.51576270
Produced by FastQC (version 0.11.5)