FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582610
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20880.3583872573419612No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA15130.25969344844750347No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13040.22382039443195278No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT12610.21643981394071504No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12370.2123204201781638No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11650.19996223889050996No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11400.1956712037211857No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10780.18502943650126155No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8580.14726832701120818No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8480.1455519129434785No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA8300.14246236762156503No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7830.13439522150323543No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG7630.13096239336777604No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCT6890.11826092926657628No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6770.11620123238530063No Hit
GTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGG6680.1146564597243439No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6520.11191019721597639No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG6020.10332812687732788No Hit
CTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAG5890.10109678858927927No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5850.1004102229621874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2600.048.8696939
TCGTATG2900.047.5092840
CGTATGC2850.047.26391641
GGTATCA12200.046.2638851
ATCTCGT2700.045.53862437
GCGTAGA6350.045.21974670
TATACAC3150.043.4624253
TATCTCG2850.043.03230736
AGCCTAT3000.042.7661632
GTATCAA36500.042.4561231
ATCTCCG3150.042.40600610
GCCTATC2800.042.28784633
GTATGCC3200.042.18054642
GAGCCTA3200.042.14858631
TACACAT3250.042.1287355
CACGAGA3100.042.04881721
GCCGTCT3250.041.83677346
TCCGGAA5350.041.14856370
CCTATCT3000.040.6918634
GTGGTAT2900.040.3074461
Produced by FastQC (version 0.11.5)