FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582610
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26660.45759599045673777No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTC19160.32886493537701034No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGGGGTACGTGCCAAGCATCCTC14690.2521412265494928No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA12190.20923087485625033No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA11840.20322342561919637No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGA10610.18211153258612106No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10190.17490259350165635No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA9100.15619368016340263No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC8770.15052951373989462No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA8290.14229072621479205No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7720.13250716602873278No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA7700.13216388321518682No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGA7580.1301041863339112No Hit
GTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGC7470.12821613085940853No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7240.1242683785036302No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6930.11894749489366814No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6290.10796244486019808No Hit
CTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGA5970.10246991984346303No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATA5960.10229827843669007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT1055.885142E-661.87211270
GTAGTTT2207.4578566E-1153.1537770
TCTATCG200.0080333950.61164531
AGGTTGT1054.3852124E-449.49769270
GGTATCA14050.041.1068461
GTATCAA36750.040.697251
GTGGTAT3700.039.7004431
TCCGGAA7050.036.85998570
TATTCTA1851.6704093E-435.11660470
GCTTATA400.003049026233.3844641
TCTAGTC400.003068337433.3414883
AAGCAAA1650.00404408831.49853170
ATCAACG47750.031.2816473
AACGCAG47800.031.2516066
TATCAAC48050.031.15842
GGGCAGT4200.030.9998591
TCAACGC48250.030.8910354
TGGTATC4650.030.8345572
CGCAGAG48650.030.7741268
CAACGCA49050.030.4551855
Produced by FastQC (version 0.11.5)