FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134339
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42600.3755491083353389No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT38440.33887576817864856No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGGGGGCTGGGAGGGCTTTG30850.2719645538062255No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30540.26923168470801057No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT28420.25054238635892795No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27100.23890565342459352No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25680.22638734981341554No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25570.2254176220688877No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25070.2210097686846701No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24690.21765980011266475No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23930.210959862968654No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23850.21025460642717916No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCT22280.19641394680073593No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21720.19147715101041224No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21700.1913008368750435No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21450.18909691018293473No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21240.18724561176156335No Hit
GTGTATGGATAAATCAGTGAGAGTGTACCCGGTGACTTTACAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCA21030.18539431334019196No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC20890.184160114392611No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTC20810.18345485785113622No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT20780.18319038664808315No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGG20450.18028120341449955No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC19440.17137733957838No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAAATCCACTTTGGTCCCAGGGCCGAAAGTGAAAGGCGTT18970.16723395739721547No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG18750.16529450190815972No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18650.1644129312313162No Hit
GTCTGGGGCTGAAGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGTAAAGTCACCGGGTACACTCTCACTGA18580.16379583175752574No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGCGAGCCGGCCTCCATCTCCTGCAACTCCAGTCAGAGCCTCCTGCA18570.16370767468984138No Hit
GTTCCATGTAGGCTGTGTTTGCAGATGTGTCCTCGGTCATGGTGAGCCTGCCCTGAAACTTCTGTGCATAAACTT18480.16291426108068224No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18180.16026954905015167No Hit
TCCATACACTGGGTGCGACAGGCTCCTGGAAAAGGCCTTGAGTGGATGGG18030.15894719303488639No Hit
GTGCAGCCACAGTTCGTTTGAAATCCACTTTGGTCCCAGGGCCGAAAGTG17590.15506828205677492No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17590.15506828205677492No Hit
ATGTTGGAGTTTATTATTGCATGCAATCTCTAGAAACGCCTTTCACTTTC17520.15445118258298446No Hit
ACATGGAACTGAGCAGCCTGACATCTGAGGACACGGCCGTCTATTATTGTACAACTCTCACTCATATTGTGA17380.1532169836354035No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17240.15198278468782261No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16940.14933807265729204No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG16650.14678151769444583No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16420.14475390513770575No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16380.14440127686696835No Hit
CTCCCTGCCCGTCACCCCTGGCGAGCCGGCCTCCATCTCCTGCAACTCCAGTCAGAGCCTCCTGCATAATAA16260.1433433920547561No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGT16170.14254997844559697No Hit
CCCCTGGCGAGCCGGCCTCCATCTCCTGCAACTCCAGTCAGAGCCTCCTGCATAATAATGGATACACCTTTT15870.1399052664150664No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15740.1387592245351698No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATGCCGTGGGGACATTGTGATGA15280.13470399942168962No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15200.13399874288021482No Hit
GTCATGGTGAGCCTGCCCTGAAACTTCTGTGCATAAACTTTTTCCCCATCTTCAGGATCAAAAAGTCCCATCCA15150.13355795754179306No Hit
GCTAATGCTCTGGGTCTCTGGATGCCGTGGGGACATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC15150.13355795754179306No Hit
TTTCTAGAGATTGCATGCAATAATAAACTCCAACATCGTCAGCCTCCACTCTACTGATTTTCAGAATAAAA15100.13311717220337133No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG14870.13108955964663122No Hit
AGCCAGGGCAGTCTCCACGGGTCCTGATCTCTTTGGGTTTTCATCGGGCCTCCGGGGTCTCCGACAGGTTCAGTG14660.1292382612252598No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14600.1287093188191537No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATGCCGTGGGGACATTGTGATGACTCAGTCTCCACTCTCC14430.12721064866851972No Hit
GTCTCCACGGGTCCTGATCTCTTTGGGTTTTCATCGGGCCTCCGGGGTCT14430.12721064866851972No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14360.12659354919472926No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14300.12606460678862316No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14240.12553566438251704No Hit
GTGTCAGTGGATCAGGCACAGATTTTATTCTGAAAATCAGTAGAGTGGAGGCTGACGATGTTGGAGTTTATTAT14230.1254475073148327No Hit
ATAATGGATACACCTTTTTGGATTGGTATTTGCAGAAGCCAGGGCAGTCTCCACGGGTCCTGATCTCTTTGGGTT14220.12535935024714834No Hit
GCATAATAATGGATACACCTTTTTGGATTGGTATTTGCAGAAGCCAGGGCAGTCTCCACGGGTCCTGATCTCTT14180.12500672197641094No Hit
GCCCTGGCTTCTGCAAATACCAATCCAAAAAGGTGTATCCATTATTATGC14050.12386068009651435No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13990.12333173769040824No Hit
GTCTCCGACAGGTTCAGTGTCAGTGGATCAGGCACAGATTTTATTCTGAAAATCAGTAGAGTGGAGGCTGACGA13470.11874757017082194No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13230.11663180054639752No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13190.11627917227566009No Hit
CTAATGGAGTACGTGATGCTTTTGATGTCTGGGGCCAGGGGACAAGGGTC13130.11575022986955398No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA13050.11504497332807918No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG13030.11486865919271047No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13030.11486865919271047No Hit
CTCTAGAAACGCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATTTCAAACGAACTGTGGCTGCACCATCTGTCT12950.11416340265123566No Hit
CTGCAAATACCAATCCAAAAAGGTGTATCCATTATTATGCAGGAGGCTCTGACTGGAGTTGCAGGAGATGGAGGC12880.1135463031774452No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12820.11301736077133909No Hit
CCATTAGTCTCCATCACAATATGAGTGAGAGTTGTACAATAATAGACGGC12750.11240026129754861No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA12670.11169500475607379No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA12570.11081343407923028No Hit
GATTTTATTCTGAAAATCAGTAGAGTGGAGGCTGACGATGTTGGAGTTTATTATTGCATGCAATCTCTAGAAACG12480.11002002047007112No Hit
CCCCTACAGAAGCCCCCAGAGCGCAGCACCTCACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAG12460.1098437063347024No Hit
GTCGGAGACCCCGGAGGCCCGATGAAAACCCAAAGAGATCAGGACCCGTGGAGACTGCCCTGGCTTCTGCAAATA12440.10966739219933372No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT12330.10869766445480583No Hit
GTATTTGCAGAAGCCAGGGCAGTCTCCACGGGTCCTGATCTCTTTGGGTTTTCATCGGGCCTCCGGGGTCTCCGA12230.10781609377796232No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATGCCGTGGGGACATTGTGATGACTCAGTCTCCAC12230.10781609377796232No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGGGGGCTGGGAGGGCTT12130.1069345231011188No Hit
GGGTCTCTGGATGCCGTGGGGACATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGCGAGCCGG12120.10684636603343445No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12080.10649373776269705No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG11980.10561216708585353No Hit
CCCTTGTCCCCTGGCCCCAGACATCAAAAGCATCACGTACTCCATTAGTCTCCATCACAATATGAGTGAGAGTTG11950.10534769588280046No Hit
TTATTATTGCATGCAATCTCTAGAAACGCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATTTCAAACGAACTG11710.10323192625837602No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11640.10261482678458557No Hit
CACCATGACCGAGGACACATCTGCAAACACAGCCTACATGGAACTGAGCAGCCTGACATCTGAGGACACGGCCG11610.1023503555815325No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11570.1019977273107951No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA11540.10173325610774203No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11530.1016450990400577No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCT11510.10146878490468898No Hit
ATCCATACACTGGGTGCGACAGGCTCCTGGAAAAGGCCTTGAGTGGATGG11470.10111615663395158No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG11450.10093984249858287No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC11420.10067537129552984No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT11390.10041090009247676No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGTA150.009348911132.180870
TATGTAA150.002515275767.867576
AATGGTA1054.419253E-756.64891470
TACCGAA200.007857950.8984412
GTGGTAT1500.049.7871171
GGTATCA6500.047.001821
TGGTATC1750.044.596732
AAGGGGG2000.043.6418769
AAAGGGG3050.042.2545270
TTCGGGG13550.040.23954870
GTATCAA16900.039.369051
TGTTAGA12050.038.66770
CTCTTAT1350.037.7175141
AGTGGTG3250.036.544085
GGGGAAA11400.035.65403770
ATCGCAC400.002809061433.95025321
GATCGCA400.002811491733.94426320
TATCAAC19550.033.498382
ATCAACG19850.032.9921043
TCTTATA1550.032.8377042
Produced by FastQC (version 0.11.5)